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Literature summary for 4.6.1.24 extracted from
- Sensing of 5 monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo (2008), Mol. Microbiol., 67, 102-115.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| nucleoside 5'-monophosphate | - |
Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| for expression in Escherichia coli BL21DE3-Gold cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D303N | mutant with amino acid substitution in the binding pocket, inactive enzyme | Escherichia coli |
| D346N | mutant with amino acid substitution in the binding pocket | Escherichia coli |
| R169A | mutant with amino acid substitution in the binding pocket, inactive enzyme | Escherichia coli |
| R169K | mutant with amino acid substitution in the binding pocket | Escherichia coli |
| T170V | mutant with amino acid substitution in the binding pocket | Escherichia coli |
| V128A | mutant with amino acid substitution in the binding pocket | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00012 | - |
5'-pGGGACAGUAUUUG-3' | wild-type | Escherichia coli | |
| 0.0081 | - |
5'-pGGGACAGUAUUUG-3' | R169K mutant | Escherichia coli | |
| 0.021 | - |
5'-OHGGGACAGUAUUUG-3' | wild-type | Escherichia coli | |
| 0.021 | - |
5'-OHGGGACAGUAUUUG-3' | R169K mutant | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA spin columns | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-OHGGGACAGUAUUUG-3' + H2O | - |
Escherichia coli | ? | - |
? | |
| 5'-pGGGACAGUAUUUG-3' + H2O | - |
Escherichia coli | ? | - |
? | |
| additional information | endonucleolytic activity is assayed using derivates of BR13, 5'-GGGACAGUAUUUG-3', a model oligonucleotide substrate for studies of RNase E and RNase G | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease G | - |
Escherichia coli |
| RNase G | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
cleavage assay | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 72 | - |
5'-OHGGGACAGUAUUUG-3' | wild-type | Escherichia coli | |
| 126 | - |
5'-pGGGACAGUAUUUG-3' | R169K mutant | Escherichia coli | |
| 180 | - |
5'-pGGGACAGUAUUUG-3' | wild-type | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
cleavage assay | Escherichia coli |
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Release 2023.1 (January 2023)
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