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Literature summary for 4.6.1.24 extracted from
- Ribonuclease T1 (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15, 435-468.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Aspergillus oryzae | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Aspergillus oryzae |  |
| iodoacetate | - |
Aspergillus oryzae | |
| Tosylglycolate | protection by substrate analogs | Aspergillus oryzae | |
| Zn2+ | - |
Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus oryzae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus oryzae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11085 | - |
primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | Aspergillus oryzae | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | Taka-diastase, commercial product of Aspergillus oryzae | Aspergillus oryzae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GpA + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + adenosine | - |
? | |
| GpG + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + guanosine | - |
? | |
| guanosine 2',3'-cyclic phosphate + H2O | - |
Aspergillus oryzae | guanosine-3'-phosphate | - |
? | |
| RNA + H2O | essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | specificity | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | Aspergillus oryzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
GpU | - |
Aspergillus oryzae | |
| 62 | - |
GpG | - |
Aspergillus oryzae | |
| 96 | - |
GpA | - |
Aspergillus oryzae | |
| 350 | - |
GpC | - |
Aspergillus oryzae | |
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Release 2023.1 (January 2023)
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