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Literature summary for 4.6.1.24 extracted from

  • Loverix, S.; Winqvist, A.; Stromberg, R.; Steyaert, J.
    Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond (2000), Chem. Biol., 7, 651-658.
    View publication on PubMed
Search for more literature for 4.6.1.24

Protein Variants

Protein Variants Comment Organism
E58A less active than the wild type enzyme Aspergillus oryzae
F100A less active than the wild type enzyme Aspergillus oryzae
H40A less active than the wild type enzyme Aspergillus oryzae
H92Q less active than the wild type enzyme Aspergillus oryzae
Y38F less active than the wild type enzyme Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information Kcat/Km values of 1000 mM-1 S-1, 0.458 mM-1 S-1, 7520 mM-1 S-1, and 8.65 mM-1 S-1 for wild type enzyme and Y38F, E58A, H92Q and F100A mutants, respectively, with GpU as substrate. Kcat/Km values of 11.3 mM-1 S-1, 15.1 mM-1 S-1, 0.153 mM-1 S-1, 27.4 mM-1 S-1, 0.117 mM-1 S-1, 1.38 mM-1 S-1 for wild type enzyme and Y38F, H40A, E58A, and F100A mutants, respectively, with SpGp(S)U as substrate. Kcat/Km values of 665 mM-1 S-1, 210 mM-1 S-1, 0.029 mM-1 S-1, 5.35 mM-1 S-1, 0.028 mM-1 S-1 and 0.508 mM-1 S-1 for wild type enzyme and Y38F, H40A, E58A, H92Q and F100A mutants, respectively, with RpGp(S)U as substrate Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
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Reaction

Reaction Comment Organism Reaction ID
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] the reaction is a transphosphorylation yielding a 2',3'-cyclophosphate. This cyclic intermediate is hydrolyzed by a water molecule to a 3'-phosphate in a separate step which is the reversal of the transphosphorylation. The transphosphorylation consists of a nucleophilic in-line inversion displacement at the phosphorous atom of the 5'-leaving group by the entering 2'-oxygen. Tyr38, His40, Glu58, His92 and Phe100 are involved in the catalysis of RNA degradation. During cyclization, Glu58 and His92 serve as the catalytic base and acid, respectively Aspergillus oryzae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GpU + H2O hydrolysis Aspergillus oryzae guanosine 3'-phosphate + uridine
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guanylyl-3',5'-uridine Transphosphorylation reaction with GpU or the diastereomers resulting from thio-substitution of a nonbridging oxygen of GpU, RpGp(S)U and SpGp(S)U as substrates. SpGp(S)U is a very poor substrate for wild type enzyme Aspergillus oryzae guanosine-2',3'-cyclic phosphate + uridine
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