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Literature summary for 4.6.1.24 extracted from

  • Shaw, K.L.; Grimsley, G.R.; Yakovlev, G.I.; Makarov, A.A.; Pace, C.N.
    The effect of net charge on the solubility, activity, and stability of ribonuclease Sa (2001), Protein Sci., 10, 1206-1215.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.24

Protein Variants

Protein Variants Comment Organism
E41K/D17K 2K mutant Aspergillus oryzae
E41K/D17K/D1K 3K mutant Aspergillus oryzae
E41K/D17K/D1K/D25K 4K mutant Aspergillus oryzae
E41K/D17K/D1K/D25K/E74K 5K mutant Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 polyinosinic acid pH 7.06, 25ÂșC, 5K mutant Aspergillus oryzae
0.1
-
 polyinosinic acid pH 7.50, 25ÂșC, 5K mutant Aspergillus oryzae
0.11
-
 polyinosinic acid pH 8.10, 25ÂșC, 5K mutant Aspergillus oryzae
0.13
-
 polyinosinic acid pH 6.50, 25ÂșC, 5K mutant Aspergillus oryzae
0.14
-
 polyinosinic acid pH 6.50, 25ÂșC, 3K mutant Aspergillus oryzae
0.14
-
 polyinosinic acid pH 7.06, 25ÂșC, 3K mutant Aspergillus oryzae
0.14
-
 polyinosinic acid pH 8.50, 25ÂșC, 5K mutant Aspergillus oryzae
0.15
-
 polyinosinic acid pH 6.50, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.16
-
 polyinosinic acid pH 6.05, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.18
-
 polyinosinic acid pH 5.60, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.18
-
 polyinosinic acid pH 6.00, 25ÂșC, 3K mutant Aspergillus oryzae
0.19
-
 polyinosinic acid pH 7.06, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.19
-
 polyinosinic acid pH 7.48, 25ÂșC, 3K mutant Aspergillus oryzae
0.2
-
 polyinosinic acid pH 8.00, 25ÂșC, 3K mutant Aspergillus oryzae
0.21
-
 polyinosinic acid pH 5.05, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.23
-
 polyinosinic acid pH 6.05, 25ÂșC, 5K mutant Aspergillus oryzae
0.24
-
 polyinosinic acid pH 4.47, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.24
-
 polyinosinic acid pH 8.48, 25ÂșC, 3K mutant Aspergillus oryzae
0.25
-
 polyinosinic acid pH 4.02, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.26
-
 polyinosinic acid pH 5.50, 25ÂșC, 3K mutant Aspergillus oryzae
0.26
-
 polyinosinic acid pH 7.50, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.29
-
 polyinosinic acid pH 8.01, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.33
-
 polyinosinic acid pH 5.00, 25ÂșC, 3K mutant Aspergillus oryzae
0.34
-
 polyinosinic acid pH 8.50, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.47
-
 polyinosinic acid pH 5.60, 25ÂșC, 5K mutant Aspergillus oryzae
0.57
-
 polyinosinic acid pH 4.50, 25ÂșC, 3K mutant Aspergillus oryzae
0.64
-
 polyinosinic acid pH 5.04, 25ÂșC, 5K mutant Aspergillus oryzae
0.75
-
 polyinosinic acid pH 4.00, 25ÂșC, 3K mutant Aspergillus oryzae
1.1
-
 polyinosinic acid pH 4.47, 25ÂșC, 5K mutant Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyinosinic acid + H2O polyI Aspergillus oryzae ?
-
 ?

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae isoelectric focusing, wild-type enzyme
-
 3.5
Aspergillus oryzae calculated from titration curve, wild-type enzyme
-
 4.2
Aspergillus oryzae calculated from titration curve, 5K mutant 4.7 4.6
Aspergillus oryzae calculated from titration curve, 2K mutant
-
 4.9
Aspergillus oryzae isoelectric focusing, 2K mutant
-
 4.9
Aspergillus oryzae isoelectric focusing, 5K mutant
-
 5.5
Aspergillus oryzae isoelectric focusing, 3K mutant
-
 6
Aspergillus oryzae calculated from titration curve, 4K mutant
-
 6.9
Aspergillus oryzae isoelectric focusing, 4K mutant
-
 6.9
Aspergillus oryzae calculated from titration curve, 3K mutant
-
 7.1