Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.3.6: arginine deiminase

This is an abbreviated version!
For detailed information about arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.6

Reaction

L-arginine
+
H2O
=
L-citrulline
 +
NH3

Synonyms

ADI, ArcA, arcA-1, arcA-2, arginine deiminase, arginine dihydrolase, arginine-degrading enzyme, citrulline iminase, deiminase, arginine, L-arginine deiminase, LADI, lymphocyte blastogenesis inhibitory factor, More, PaADI, PAD, PAD2, peptidylarginine deiminase, PpADI, Streptococcal acid glycoprotein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.6 arginine deiminase

Purification

Purification on EC 3.5.3.6 - arginine deiminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
16.92fold purification, 49.17% yield
-
DEAE-cellulose column chromatography and butyl-Sepharose column chromatography
DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, hydroxyapatite column chromatography, and ammonium sulfate precipitation
-
deiminase I and II
-
Hi-Trap chelating Sepharose column chromatography and Superdex 200 gel filtration
native enzyme by protamine sulfate treatment, and gel filtration
-
native enzyme, and recombinant enzyme from Escherichia coli
-
native enzyme, and recombinant enzyme from Escherichia coli strain BL21(DE3), recombinant selenomethionine-labeled enzyme
-
native enzyme, and recombinant His-tagged enzyme from Escherichia coli by metal chelating affinity chromatography in a single step
-
native enzyme, multistep procedure, to homogeneity
-
Ni+-NTA resin column chromatography, ultrafiltration, and gel filtration
-
nickel-chelated agarose gel column chromatography
Q-Sepharose column chromatography and Sephacryl-S200 gel filtration
recombinant ADI truncation mutant from Escherichia coli inclusion bodies to homogeneity
-
recombinant enzyme
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21 by anion exchange chromatography and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by sequential anion exchange chromatography and gel filtration to near homogeneity
recombinant protein
-
recombinant wild-type and mutant enzyme
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) inclusion bodies
-
StrepTactin Sepharose column chromatography
Super-Q column chromatography and Toyoperl HW-55S gel filtration
-