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3.5.3.6: arginine deiminase

This is an abbreviated version!
For detailed information about arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.6

Reaction

L-arginine
+
H2O
=
L-citrulline
 +
NH3

Synonyms

ADI, ArcA, arcA-1, arcA-2, arginine deiminase, arginine dihydrolase, arginine-degrading enzyme, citrulline iminase, deiminase, arginine, L-arginine deiminase, LADI, lymphocyte blastogenesis inhibitory factor, More, PaADI, PAD, PAD2, peptidylarginine deiminase, PpADI, Streptococcal acid glycoprotein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.6 arginine deiminase

Specific Activity

Specific Activity on EC 3.5.3.6 - arginine deiminase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000345
-
crude extract, pH 9.5, at 37°C
0.115
-
purifed recombinant enzyme
0.175
-
after purification, pH 9.5, at 37°C
0.476
crude enzyme extract, IU/mg dry cell weight
1.5
purified enzyme, at pH 6.5 and 37°C
12.9
-
pH 8.0, 37°C
126.5
substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/F269Y
131.2
substrate: L-arginine, pH 6.0, 45°C, wild-type enzyme
134.7
substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/G264P
140.3
143.2
substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K
16.75
pH 7.4, 37°C, mutant D38H/A128T/E296K/H404R/I410L
18
-
pH 7.0, 45°C, enzyme covalently immobilized on dextran
21.5
-
pH 7.0, 45°C, PEGylated enzyme
25.5
-
PEG-modified enzyme
26.7
-
pH 7.0, 45°C, soluble enzyme
27
-
free enzyme
296.8
substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/G292P
32.7 - 37
-
purified recombinant enzyme
332.8
substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/F269Y/G292P
35
-
purified enzyme
4.76
at pH 6.0 and 37°C
40
-
purifed recombinant ADI mutant
41
-
purified native enzyme
44.5
-
purified native enzyme
5.4
-
purified enzyme
50
-
purified rcombinant enzyme
7.2
pH 7.4, 37°C, mutant enzyme A128T/H404/I410L/D38H/E298K/Q162G
70.3
pH and temperature not specified in the publication
76.65
-
pH 5.5, 37°C
8.34
pH 7.4, 37°C, mutant enzyme A128T/H404/I410L/D38H/E298K/Q162T
8.53
pH 7.4, 37°C, mutant enzyme A128T/H404/I410L/D38H/E298K/Q162S
8.75
pH 7.4, 37°C, mutant enzyme A128T/H404/I410L/D38H/E298K
9.92
pH 7.4, 37°C, mutant A128T/H404R/I410L
93.4
pH 7.4, 37°C, mutant D38H/A128T/E296K/H404R/I410L/A276W
additional information