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3.5.3.6: arginine deiminase

This is an abbreviated version!
For detailed information about arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.6

Reaction

L-arginine
+
H2O
=
L-citrulline
 +
NH3

Synonyms

ADI, ArcA, arcA-1, arcA-2, arginine deiminase, arginine dihydrolase, arginine-degrading enzyme, citrulline iminase, deiminase, arginine, L-arginine deiminase, LADI, lymphocyte blastogenesis inhibitory factor, More, PaADI, PAD, PAD2, peptidylarginine deiminase, PpADI, Streptococcal acid glycoprotein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.6 arginine deiminase

pH Range

pH Range on EC 3.5.3.6 - arginine deiminase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7
4.5 - 7.75
-
a series of in vitro batch fermentations using reconstituted Man-Rogosa-Sharpe (MRS) medium at different constant pH values (pH 4.50 - pH 7.75) is performed. Arginine conversion through the ADI pathway, which is activated from the stationary growth phase on, results in the production of both citrulline and ornithine for all pH conditions tested. The pattern and the ratio of the end-products of the ADI pathway are influenced by pH
5 - 6.5
-
for certain pH values (between pH 5.0 and 6.5), a further conversion of citrulline into ornithine is found when all arginine is depleted
5 - 7
-
deviations from the optimal pH (pH 5.0 and pH 7.0) results in an overall decline of the relative expression level of arginine deiminase
5 - 7.5
5 - 8.6
pH profiles of wild-type and mutant enzymes
5.5 - 9
the kcat value is maximal from pH 5.5 to pH 9.0
6 - 11
-
38.5% activity remaining at pH 6.0 and 3.4% activity remaining at pH 11.0
6 - 7.4
-
pH 6.0: maximal activity, pH 7.4: 13% of maximal activity
6 - 8
the kcat_Km value is maximal between pH 6.0 and 8.0
6 - 9.5
-
pH 6.0: about 50% of maximal activity, pH 9.5: about 55% of maximal activity, soluble enzyme
6.4 - 7.8
-
pH 6.4: about % of maximal activity, pH 7.8: about % of maximal activity
6.5 - 7.5
-
a pH shift from 6.5 to 7.5 results in an approximately 80% drop in activity