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Literature summary for 3.5.3.6 extracted from

  • Misawa, S.; Aoshima, M.; Takaku, H.; Matsumoto, M.; Hayashi, H.
    High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme (1994), J. Biotechnol., 36, 145-155.
    View publication on PubMed
Search for more literature for 3.5.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in the cytosol of Escherichia coli Mycoplasmopsis arginini

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Arg + H2O Mycoplasmopsis arginini the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplasmopsis arginini
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mycoplasmopsis arginini

Renatured (Commentary)

Renatured (Comment) Organism
renaturation after treatment with 6 M guanidine hydrochloride: optimum renaturation conditions are incubation for 90 h at pH 7.5 and 15°C Mycoplasmopsis arginini

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.6
-
-
 Mycoplasmopsis arginini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg + H2O
-
 Mycoplasmopsis arginini L-citrulline + NH3
-
 ?
L-Arg + H2O the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A Mycoplasmopsis arginini citrulline + NH3
-
 ?