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3.5.3.6: arginine deiminase

This is an abbreviated version!
For detailed information about arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.6

Reaction

L-arginine
+
H2O
=
L-citrulline
 +
NH3

Synonyms

ADI, ArcA, arcA-1, arcA-2, arginine deiminase, arginine dihydrolase, arginine-degrading enzyme, citrulline iminase, deiminase, arginine, L-arginine deiminase, LADI, lymphocyte blastogenesis inhibitory factor, More, PaADI, PAD, PAD2, peptidylarginine deiminase, PpADI, Streptococcal acid glycoprotein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.6 arginine deiminase

Molecular Weight

Molecular Weight on EC 3.5.3.6 - arginine deiminase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
gel filtration
100000 - 110000
-
gel filtration
105000
120000
127000
gel filtration
130000
-
gel filtration
140000
150000 - 160000
-
gel filtration
199000
-
gel filtration
44000
-
2 * 44000, the subunits differ in conformation, SDS-PAGE
45000
45900
45920
-
electrospray mass spectrometry
46000
46050
-
calculated from amino acid sequence
46500
deduced ADI protein sequence
46940
47000
-
2 * 47000, SDS-PAGE
47200
x * 47200, SDS-PAGE
48000
-
2 * 48000, SDS-PAGE
49000
51000
-
purified recombinant truncated PAD (missing 43 N-terminal amino acid residues but including Xpress and poly-His tags), SDS-PAGE
53000
-
active enzyme, gel filtration
54000
-
2 * 54000, SDS-PAGE
55000
-
2 * 55000, cetyl trimethylammonium bromide
60880
-
truncated PAD with its poly-His and Xpress epitope tags, calculated from amino acid sequence
60890
-
MALDI-TOF mass spectrometry
64189
2 * 64189, calculated from amino acid sequence
64190
2 * 64190, ESI-MS analysis
66000
2 * 66000, SDS-PAGE
80000
-
gel filtration
87000
-
gel filtration
87300
-
equilibrium sedimentation
90000
94365
-
MALDI-TOF
95600
-
non-denaturing PAGE
additional information
-
genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster