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3.5.3.6: arginine deiminase

This is an abbreviated version!
For detailed information about arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.6

Reaction

L-arginine
+
H2O
=
L-citrulline
 +
NH3

Synonyms

ADI, ArcA, arcA-1, arcA-2, arginine deiminase, arginine dihydrolase, arginine-degrading enzyme, citrulline iminase, deiminase, arginine, L-arginine deiminase, LADI, lymphocyte blastogenesis inhibitory factor, More, PaADI, PAD, PAD2, peptidylarginine deiminase, PpADI, Streptococcal acid glycoprotein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.6 arginine deiminase

Crystallization

Crystallization on EC 3.5.3.6 - arginine deiminase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion technique at 23°C, apoPAD2 crystals with increasing concentrations of calcium (0-10 mM), 16 structures are solved at eight different calcium concentrations to 1.66-1.97 A
apo-enzyme, sitting drop vapor diffusion method, using 0.9 M trisodium citrate, 0.1M sodium cacodylate pH 6.5
crystal structure determination and analysis of purified recombinant enzyme at 1.6 and 2.0 A resolution, respectively
-
in complex with substrate
catalytically important residues are C406 and H278
-
crystal structure determination and analysis of purified recombinant enzyme at 2.45 A resolution, recombinant selenomethionine-labeled enzyme by sitting drop vaour diffusion method
-
mutants C406A, H287A, D280A, and D166A in complex with L-arginine, protein solutions containing 20 mM arginine are mixed with an equal volume of mother liquor containing 34-38% 2-methyl-2,4-pentanediol, 6.0-7.0% PEG 3350, 0.1 M Tris-HCl, pH 7.6, and 20 mM arginine, equilibration against the mother liquor reservoir, 2-8 weeks, X-ray diffraction structure determination and anaylsis at 2.3-2.9 A resolution, modeling
X-ray crystallographic coordinates for the structure of the C406A ADI -L-arginine complex, overview