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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
 +
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

Inhibitors

Inhibitors on EC 3.5.1.11 - penicillin amidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-alpha-ethylphenylacetic acid
-
-
(R)-alpha-hydroxyphenylacetic acid
-
-
(R)-alpha-methylphenylacetic acid
-
-
(R)-phenylglycinamide
-
-
(R)-phenylglycine
-
-
(S)-alpha-ethylphenylacetic acid
-
-
(S)-alpha-hydroxyphenylacetic acid
-
-
(S)-alpha-methylphenylacetic acid
-
-
2,3-Butanedione
-
50 mM, 78% loss of activity, benzylpenicillin or phenylacetate protects
2-hydroxy 5-nitrobenzylbromide
-
-
2-thienylacetic acid
-
competitive inhibitor
6-aminopenicillanate
-
product inhibition
6-aminopenicillanic acid
7-amino-3-desacetoxycephalosporanic acid
-
non-competitive inhibitor
8-hydroxyquinoline
-
complexed with zinc, reactivation by addition of ZnSO4, MnSO4, MgSO4, CoSO4, FeSO4
acetone
-
upon permeabilization
benzothiophene-2-boronic acid
-
fast reversible competitive inhibitor
benzyl penicillin
-
-
Benzylisocyanate
-
-
Ca2+
inhibits 15.1% at 1 mM
cephalosporin C
65% inhibition; 65% inhibition at 1 mM
cephalosporin G
79% inhibition; 79% inhibition at 1 mM
deoxycholic acid
slight inhibition
diethyldicarbonate
Pseudomonas melanogenum
-
-
dimethylformamide
inhibits 10.5% at 10% and 33.2% at 40%
EDTA
inhibits 80.4% at 10 mM
Fe3+
inhibits 96.0% at 1 mM
glycodeoxycholic acid
slight inhibition
Hg2+
Pseudomonas melanogenum
-
decreases enzyme activity to 50%
N-bromosuccinamide
-
0.1 mM, 70% loss of activity
N-bromosuccinimide
Na+
activity is slightly enhanced at 0.6 M NaCl, reaches 100% activity at 1 M NaCl, and is gradually inhibited by higher NaCl concentrations, retaining 60% activity at 3 M NaCl
penicillin G
the enzyme is covalently immobilized on aminopropyl functionalized mesocellular foam silica and is further cross-linked using glutaraldehyde without deactivation and upto 95% efficiency. The resulting biocatalyst demonstrates improved resistance to the substrate and product inhibition
phenoxyacetic acid
phenylacetamide
-
-
phenylacetic acid
Phenylglyoxal
-
20 mM, 82% loss of activity, benzylpenicillin or phenylacetate protects
phenylmethanesulfonic acid N-hydroxysuccinimide ester
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
Phenylmethylsulfonyl azide
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
Phenylmethylsulfonyl chloride
-
reactivation by incubation with 6-aminopenicillanic acid or proteins from E. coli
phenylmethylsulfonyl fluoride
Phenylmethylsulfonylfluoride
-
-
PMSF
-
irreversible, used for active site titrations of the cross-linked enzyme aggregates
Pyridine
-
upon permeabilization
Sr2+
inhibits 95.4% at 1 mM
taurodeoxycholic acid
slight inhibition
Urea
inhibits 95.7% at 8 M, no inhibition at 4 M
[bmim]dca
-
the enzyme activity in 25% [bmim]dca is 1.6fold than activity in water. The enzyme activity is decreased in higher concentration of [bmim]dca
additional information
-