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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
 +
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

KM Value

KM Value on EC 3.5.1.11 - penicillin amidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
2-benzoxazolon-3-yl-acetic acid methyl ester
-
pH 6.8, 25°C
0.63
2-nitro-5-(phenoxyacetamido)-benzoic acid
pH 6.6, 40°C
1.6
2-nitro-5-phenoxyacetamide benzoic acid
-
0.081
2-nitro-5-phenylacetamidobenzoic acid
-
pH 7.5, 25°C
0.00614 - 0.03
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
0.0005 - 30.39
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
0.147
2-phenyl-N-(4-sulfamoylphenyl)acetamide
-
pH 7.0, 25°C
0.00063
2-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.0004
3-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.027 - 0.114
4-Hydroxyphenylacetamide
0.031
4-nitrophenyl acetate
-
-
0.0003
4-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.07 - 0.23
5-nitro-3-[(phenylacetyl)amino]benzoic acid
0.0083 - 0.0197
6-nitro-3-(phenylacetamido)-benzoic acid
0.0176
6-nitro-3-phenylacetamide benzoic acid
-
pH 7.5, 37°C
0.012 - 0.02
6-nitro-3-phenylacetamidobenzoic acid
0.000015 - 0.027
6-nitro-3-[(phenylacetyl)amino]benzoic acid
0.01
7-phenylacetamidodeacetoxycephalosporanic acid
-
-
0.399 - 41.26
amoxicillin
0.105
amoxycillin
pH 7.4, 37°C, recombinant enzyme
0.0944 - 5.2
ampicillin
0.0031 - 4.5
benzylpenicillin
2
benzylpenicilloic acid
-
-
0.0519
carbenicillin
pH 7.4, 37°C, recombinant enzyme
0.284 - 0.646
cefadroxil
1.79
cephadrine
Pseudomonas melanogenum
-
-
0.0255 - 2.1
cephalexin
1.96
cephaloglycin
Pseudomonas melanogenum
-
-
0.0164 - 0.1
cephaloridine
0.0156 - 0.042
cephalothin
3.2
D-(-)-alpha-aminophenylacetic acid p-nitroanilide
-
-
0.646 - 1.3
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid
9.1 - 12.2
D-4-hydroxyphenylglycine amide
1.6723 - 51.15
D-hydroxyphenylglycine methyl ester
-
2 - 30
D-phenylglycine amide
0.045
ethyl 2-phenylacetate
-
-
-
0.153
ethyl 4-[(phenylacetyl)amino] benzoate
-
pH 7.0, 25°C
0.137
N,2-diphenylacetamide
-
pH 7.0, 25°C
0.23
N-(4-acetylphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.171
N-(4-bromophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.16
N-(4-cyanophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.162
N-(4-methoxyphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.121
N-(4-methylphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.095
N-(4-nitrophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.00051
N-(5-nitro-2-pyridyl)-phenylacetamide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.159
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
-
pH 7.0, 25°C
0.8
N2-phenylacetyl-2'-deoxyguanosine
-
pH 7.8, 25°C
0.46
N6-phenylacetyl-2'-deoxyadenosine
-
pH 7.8, 25°C
0.82 - 5.6
penicillin dihydroF
1.39 - 15.1
penicillin F
0.00001 - 164
penicillin G
0.05 - 1
penicillin K
0.0393 - 40
Penicillin V
0.0002 - 0.156
phenylacetamide
0.0004
phenylacetate 4-nitroanilide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.097
phenylacetate p-nitroanilide
-
-
0.0002
phenylacetyl-2-naphthylamide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.4
phenylacetyl-3-aminobenzoic acid
-
-
0.18
phenylacetyl-4-aminobenzoic acid
-
-
0.000012
phenylacetyl-7-amido-4-methylcoumarin
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.95
phenylacetyl-anthranilic acid
-
-
5.2
phenylacetyl-Gly
-
pH 7.8, 25°C
0.67
phenylacetylaspartame
-
pH 7.8, 25°C
0.08
phenylacetylglycine
-
-
additional information
additional information
-