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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
+
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

pH Stability

pH Stability on EC 3.5.1.11 - penicillin amidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
the enzyme is functionally stable in the pH range 3-7 with than 80% retention in activity after 4 h. Significant reduction in activity at pH 9-11, with only 40% of the original activity retained. The enzyme is rapidly inactivated at pH 1-2
753864
3 - 9.5
-
the enzyme maintains the folded structure in the pH range from pH 3.0 to 9.5
685398
3.9 - 10.7
-
pH-stability studies of recombinant wild-type and mutant enzymes, inactivation above pH 10.7 and below pH 3.9, overview
713467
4 - 10
stable
755333
4 - 4.8
-
high inactivation at pH 4.0, but stable at pH 4.8
711120
4.5 - 4.8
-
high inactivation at pH 4.5, but stable at pH 4.8
711120
5
purified recombinant enzyme, 4 h at room temperature, over 50% activity remaning
719605
5 - 12
10 min at 40°C, more than 50% of the activity is retained
719605
5 - 9
6 - 8
purified recombinant enzyme, 4 h at room temperature, completely stable at
719605
6 - 9
purified recombinant His-tagged enzyme, 30°C, 30 min, over 80% activity within this range
733557
6.5 - 10
-
highest stability when incubated at 24°C for 9 h
680527
7 - 10.5
stable between pH 7.0 and 10.5, recombinant enzyme
752541
9.1
-
half-life at 25°C is 9.1 h
667754
additional information
-
study on inactivation kinetics over a wide pH-range at 25°C and 50°C. Enzyme is very stable in neutral solutions and quickly loses its catalytic activity in acidic and alkaline solutions. Inactivation proceeds according to first order reaction kinetics
667754