Crystallization (Comment) | Organism |
---|---|
both wild-type and mutants F146Y, F24Y, F146Y/F24Y | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F146Y | alpha-subunit, increased affinity for Calpha-substituted substrates | Escherichia coli |
F146Y/F24A | mutation F24Y in beta-, F146Y in alpha-subunit, increased affinity for Calpha-substituted substrates | Escherichia coli |
F24A | beta-subunit, increased affinity for Calpha-substituted substrates, 20fold reduced affinity for phenylacetic acid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-alpha-ethylphenylacetic acid | - |
Escherichia coli | |
(R)-alpha-hydroxyphenylacetic acid | - |
Escherichia coli | |
(R)-alpha-methylphenylacetic acid | - |
Escherichia coli | |
(R)-phenylglycinamide | - |
Escherichia coli | |
(R)-phenylglycine | - |
Escherichia coli | |
(S)-alpha-ethylphenylacetic acid | - |
Escherichia coli | |
(S)-alpha-hydroxyphenylacetic acid | - |
Escherichia coli | |
(S)-alpha-methylphenylacetic acid | - |
Escherichia coli | |
phenylacetamide | - |
Escherichia coli | |
phenylacetic acid | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
penicillin + H2O = a carboxylate + 6-aminopenicillanate | F24 in beta-subunit has a fixed position, whereas F146 of alpha-subunit acts as a flexible lid on the binding site | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-nitro-5[(phenylglycyl)amino]benzoic acid + H2O | - |
Escherichia coli | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
phenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
0.05 | - |
phenylacetamide | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
0.06 | - |
phenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
0.076 | - |
(R)-alpha-methylphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
0.124 | - |
(R)-alpha-methylphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
0.2 | - |
phenylacetamide | wild-type, pH 7.0, 30°C | Escherichia coli | |
0.2 | - |
(R)-alpha-methylphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
0.276 | - |
(R)-alpha-ethylphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
0.36 | - |
(S)-alpha-methylphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
0.37 | - |
(S)-alpha-ethylphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
0.4 | - |
(R)-alpha-ethylphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
0.47 | - |
(R)-alpha-methylphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
0.5 | - |
(S)-alpha-methylphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
1 | - |
(S)-alpha-ethylphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
1 | - |
phenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
1.1 | - |
(S)-alpha-methylphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
1.1 | - |
phenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
1.1 | - |
(S)-alpha-ethylphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
1.5 | - |
phenylacetamide | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
1.5 | - |
(S)-alpha-methylphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
2 | 5 | (R)-phenylglycinamide | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
2 | - |
phenylacetamide | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
2.1 | - |
(S)-alpha-ethylphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
3.3 | - |
(R)-alpha-ethylphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
3.5 | - |
(R)-alpha-hydroxyphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
4 | - |
(R)-phenylglycinamide | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
4.05 | - |
(S)-alpha-hydroxyphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
5.7 | - |
(R)-alpha-ethylphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
5.7 | - |
(S)-alpha-hydroxyphenylacetic acid | mutant F24A, pH 7.0, 30°C | Escherichia coli | |
6.2 | - |
(R)-alpha-hydroxyphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
8.2 | - |
(S)-alpha-hydroxyphenylacetic acid | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
10 | - |
(S)-alpha-hydroxyphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
13 | - |
(R)-alpha-hydroxyphenylacetic acid | mutant F146Y, pH 7.0, 30°C | Escherichia coli | |
15 | - |
(R)-phenylglycinamide | mutant F146Y/F24A, pH 7.0, 30°C | Escherichia coli | |
31 | - |
(R)-alpha-hydroxyphenylacetic acid | wild-type, pH 7.0, 30°C | Escherichia coli | |
40 | - |
(R)-phenylglycinamide | wild-type, pH 7.0, 30°C | Escherichia coli |