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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
 +
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

Activating Compound

Activating Compound on EC 3.5.1.11 - penicillin amidase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-butanone
5% (v/v), 30 min at room temperature, 1.74fold activation
2-mercaptoethanol
1 mM, 1.19fold activation
acetonitrile
5% (v/v), 30 min at room temperature, 1.65fold activation
chloroform
-
cell-bound PVA activity shows an eightfold increase upon treatment with chloroform at 0.005 ml/mg dry biomass for 10 min upon permeabilization
CTAB
0.1%, 2.5fold activation
Dichloromethane
-
enhances the enzyme activity up to 2fold upon permeabilization
diethyl ether
-
cell-bound PVA activity shows an eightfold increase upon treatment with diethylether at 0.010 ml/mg dry biomass for 45 min upon permeabilization
dithiothreitol
10 mM, 1.23fold activation
ethyl acetate
hexane
Isopropanol
5% (v/v), 30 min at room temperature, 1.95fold activation
Toluene
-
enhances the enzyme activity up to 6fold upon permeabilization
Triton X-100
0.1%, 1.8fold activation
Tween 20
0.1%, 1.75fold activation
[bmim]dca
-
the enzyme activity in 25% [bmim]dca is 1.6fold than activity in water. The enzyme activity is decreased in higher concentration of [bmim]dca
additional information
-
addition of chemically synthesized fragments of the pro-peptide to purified mature enzyme increases its specific activity up to 2.3fold
-