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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
 +
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

Cloned

Cloned on EC 3.5.1.11 - penicillin amidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from K. citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli
-
a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from Kluyvera citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli
-
a penicillin G acylase gene from Alcaligenes faecalis and a pcm gene encoding protein isoaspartate methyltransferase (PIMT) are constructed into pET43.1a(+) and pET28a(+), respectively. The recombinant plasmids pETAFPGA and pETPCM are transformed into the same host cell Escherichia coli BL21 (DE3). The two plasmids can exist in the host cell and the two genes can be efficiently expressed after induction. The product of pcm gene can function as a helper molecule for penicillin G acylase. PIMT increases the enzymatic activities in supernatant of ferment broth (1.6folds) and cell lysate (1.8folds), while it does not significantly affect the expression level of penicillin G acylase
-
cloned into pUC 9 and transformed into Escherichia coli
-
expressed in Bacillus megaterium MS941, strain is deficient in the major extracellular protease NprM due to deletion of the corresponding gene, expressed in the newly constructed Bacillus megaterium YYBm1 strain, which has a xylose utilization deficiency, 7fold higher enzyme expression compared with host strain
-
expressed in enzyme deficient Bacillus megaterium strain UN-cat, 20fold higher expression rate than in host strain
-
expressed in Escherichia coli
-
expressed in Escherichia coli and Bacillus subtilis, high expression rate of extracellular enzyme in Bacillus subtilis
-
expressed in Escherichia coli BL21(DE3) using the pET-26b(+) expression vector
expressed in Escherichia coli HB101, enzyme is expressed as cytoplasmic enzyme, enzyme production not inducible by phenylacetic acid, expressed in Escherichia coli TG1, enzyme production inducible by addition of IPTG, intracellular expression
expressed in Escherichia coli HB101, sequence shows one mutation resulting in a betaVal148Leu substitution in the mature protein as compared with the Swiss-Prot entry P06875 derived from Escherichia coli ATC11105
expressed in Escherichia coli MC1061
-
expressed in Escherichia coli, attaining a very high yield (250 mg/l) and a comparatively high specific activity (430 IU/mg)
expressed in Streptomyces lividans
expression in a phage display in Escherichia coli strain TG1
-
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli strain BL21 pLys (DE3)
expression in Providencia pastoris strain LN5.5 with secretion to the culture medium
-
expression in Streptomyces lividans
-
expression levels in various Escherichia coli host cells. Intracellular proteolytic degradation of the newly synthesized penicillin amidase precursor and translocation through the plasma membrane are determined to be the main posttranslational processes limiting enzyme production. The production of mature active penicillin amidase is increased up to 10fold when the protease deficient strain Escherichia coli BL21 (DE3) is cultivated in medium without a proteinaceous substrate. Simultaneous coexpression of the OmpT pac gene with some proteins of the Sec export machinery of the cell results in up to threefold-enhanced penicillin amidase production
-
expression of functional wild-type and mutant enzymes in Pseudomonas aeruginosa PAO1 and PAO1DELTAtatABC strains using the pMMB67EH shuttle vector, the mutant a TatProPGA hybrid only shows an active site, when the transformed bacterium is grown at 25°C or lower temperatures. Processing of recombinant PGA can also occur in the cytoplasm of Pseudomonas aeruginosa. The extracellular localization of the TatProPGA hybrid is not dependent on the tatABC-genes
-
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
-
expresssion of the enzyme in Escherichia coli strain 5KpHM12
-
gene pac, overexpression in Escherichia coli strain HB101 and secretion of the enzyme to the culture medium, method optimization, overview
-
gene pac, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene pac, recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) pLysS
gene PGA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, cloning and recombinant expression of His-tagged enzyme in Escherichia coli strain M15
gene yxeI, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3)
native and mutant proteins expresses in Bacillus subtilis WB600
-
PGA gene cloning isolated from environmental and clinical samples, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, overexpression as C-terminally His-tagged protein in Escherichia coli strain BL21 under control of the T7 promoter using pGEM -T easy vector
production of penicillin amidase hybrid I and hybrid II in Escherichia coli. Hybrid A contains A-chain from K. citrophila and B-chain from Escherichia coli. Hybrid II contains A-chain from Escherichia coli and B-chain from Kluyvera citrophila
-
production of penicillin amidase hybrid I and hybrid II in Escherichia coli. Hybrid A contains A-chain from Kluyvera citrophila and B-chain from Escherichia coli. Hybrid II contains A-chain from Escherichia coli and B-chain from Kluyvera citrophila
-
recombinant expression in Escherichia coli strain BL21(DE3)
the heterologous expression of pva in Streptomyces lividans leads to the production of an extracellularly homogeneous heterodimeric enzyme at a 5fold higher concentration than in the original host and in a considerably shorter time
the pva gene is cloned into a pET28b plasmid vector between NcoI and XhoI restriction sites. The PVA enzyme is expressed in Escherichia coli BL21 star cells