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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
 +
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

Purification

Purification on EC 3.5.1.11 - penicillin amidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
commercial preparation
-
commercial preparation, Eupergit-immobilized
-
from cell culture medium of transgenic Bacillus subtilis
-
His-tagged enzyme expressed in Escherichia coli
-
His-tagged recombinant enzyme
hydrophobic interaction chromatography
ion exchange chromatography (Q-Sepharose)
mixed ion exchange supports are useful ion exchangers for purification of penicillin G acylase
-
mutant proenzyme T263G, expressed in Escherichia coli
-
native and mutant proteins
-
native enzyme 12.7fold from fermentation broth by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview
-
native enzyme 4.5-7.5fold from crude cell extract or homogenate by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview
-
partially
periplasmic fraction
purification of His-tagged enzyme secreted into periplasmic psace by immobilized metal-ion affnity chromatography. Maximum specific activity of enzyme is 38.5 U/mg, yield is 70%
-
recombinant enzyme
recombinant enzyme expressed in Escherichia coli
-
recombinant enzyme from Bacillus megaterium UN-cat
-
recombinant enzyme from Escherichia coli strain 5KpHM12 culture supernatant by anion and cation exchange chromatographic high-throughput absorption membranes, method development and evaluation, binding dynamics, overview
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by a single-step anion-exchange chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat shock treatment of 20 min at 65°C, and immobilized metal ion affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction chrmatography and ultrafiltration
recombinant enzyme from Providencia pastoris strain LN5.5 by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography
-
recombinant extracellular enzyme 3.2fold from Escherichia coli strain HB101 culture supernatant by anion exchange chromatography, single-step purification
-
recombinant His-tagged enzyme from Escherichia coli strain M15 by affinity chromatography and gel filtration
recombinant N-terminally His-tagged wild-type and mutant enzymes to homogeneity from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli BL21(DE3) to homogeneity
-
wild-type enzyme and pro-peptide mutants T206G, T206P, T206G/G213G and T206G/S213G/T219G
-