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3.5.1.11: penicillin amidase

This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.

Word Map on EC 3.5.1.11

Reaction

penicillin
+
H2O
=
a carboxylate
+
6-aminopenicillanate

Synonyms

ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.11 penicillin amidase

Molecular Weight

Molecular Weight on EC 3.5.1.11 - penicillin amidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
-
gel filtration
120000
137000
-
gel filtration
140000
-
gel filtration
146000
Pseudomonas melanogenum
-
gel filtration
148000
gel filtration
154000
gel filtration
20500
22100
-
alpha,beta, x * 22100 + x * 63600, SDS-PAGE
22540
-
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI
22920
-
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI
23000
24000
-
x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE
24500
-
1 * 24500, alpha-subunit, + 1 * 65000, beta-subunit, SDS-PAGE, the subunits are held together by noncovalent forces
25500
x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE
26000
1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE
26900
-
1 * 26900 + 1 * 59100, SDS-PAGE
27000
27753
-
1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence
37008
40000
-
x * 40000, SDS-PAGE
57000
59100
-
1 * 26900 + 1 * 59100, SDS-PAGE
61394
-
1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence
61900
x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE
62000
62400
x * 27000, alpha-subunit, + x * 62400, beta-subunit, SDS-PAGE
62564
-
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI
62568
-
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI
63000
1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE
63600
-
alpha,beta, x * 22100 + x * 63600, SDS-PAGE
65000
-
1 * 24500, alpha-subunit, + 1 * 65000, beta-subunit, SDS-PAGE, the subunits are held together by noncovalent forces
69000
70000
71000
-
sedimentation equilibrium
72000
Pseudomonas melanogenum
-
2 * 72000, SDS-PAGE
83200
-
gel filtration
88000
recombinant His-tagged enzyme, gel filtration
92000
1 * 92000, pro-enzyme, SDS-PAGE