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3.1.1.75: poly(3-hydroxybutyrate) depolymerase

This is an abbreviated version!
For detailed information about poly(3-hydroxybutyrate) depolymerase, go to the full flat file.

Word Map on EC 3.1.1.75

Reaction

[(R)-3-hydroxybutanoate]n
+
H2O
=
[(R)-3-hydroxybutanoate]n-x
 +
[(R)-3-hydroxybutanoate]x

Synonyms

AFUA_4G03560, i-PHA depolymerase, intracellular poly(3-hydroxybutyrate) depolymerase, intracellular poly(3-hydroybutyrate) depolymerase, MCL-PHA depolymerase, medium chain length polyhydroxyalkanoate depolymerase, P(3HB) depolymerase, P(3HB) depolymerase A, P(3HB) depolymerase B, P(3HB-co-3HV) depolymerase, P(3HV) depolymerase, PDI, PHA depolymerase, PHAase I, PHAase II, PhaZ, PhaZ-Th, PhaZ1, PhaZ1-Z6, phaZ3, PhaZ5, PhaZ6, PhaZ7, PhaZ7 depolymerase, PhaZa1, PhaZBm, PhaZd, PhaZd1, PhaZd2, PhaZRpiT1, PhaZSa, PHB depolymerase, PHB depolymerase A, PHB depolymerase inhibitor, PHB-depolymerase, PHBDP, PHV depolymerase, poly (beta-hydroxybutyrate) depolymerase, poly(3-hydroxybutyrate) (PHB) depolymerase, poly(3-hydroxybutyrate) depolymerase, poly(3-hydroxyvalerate) depolymerase, poly(3-hydroxyvaleric acid) depolymerase, poly(3HB) depolymerase, poly(beta-hydroxybutyrate) depolymerase, poly(HA SCL) depolymerase, poly(HA) depolymerase, poly(hydroxybutyric) depolymerase, poly-beta-hydroxybutyrate depolymerase, poly-beta-hydroxybutyric acid depolymerase, polyhydroxyalkanoate depolymerase, polyhydroxybutyrate depolymerase, poly[(R)-3-hydroxybutyrate] depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly[D(-)-3-hydroxybutyrate] depolymerase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.75 poly(3-hydroxybutyrate) depolymerase

Purification

Purification on EC 3.1.1.75 - poly(3-hydroxybutyrate) depolymerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2step chromatography
-
affinity chromatography
-
expression in Escherichia coli
extracellular enzyme by gel filtration and adsorption chromatography
extracellular native enzyme from culture supernatant 2.6fold
-
extracellular native enzyme from culture supernatant 5.6fold
-
His-tag fusion, Ni-affinity chromatography
-
His-tag, Ni-affinity chromatography
-
native and recombinant protein
-
native enzyme 401fold by ammonium sulfate fractionation and gel filtration
-
native extracellular enzyme 20.62fold by acetone precipitation and gel filtration
-
PhaZ6 protein, to homogeneity, chromatography steps
-
PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75
-
purification from inclusion bodies
-
purification of His-tagged PhaZ and its variant in Escherichia coli JM109
-
recombinant enzyme from Rhodococcus sp. strain T104 cell culture medium to homogeneity
-
recombinant enzyme with a C-terminal His6 tag, Ni-Sepharose Fast Flow column, SDS-PAGE
recombinant His-tagged wild-type and mutant enzymes from Bacillus subtilis strain WB800 by affinity chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by nicke affinity chromatography
recombinant protein
recombinant soluble His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
recombinant wild-type and mutant extracellular enzyme from Escherichia coli strain BL21(DE3) and Rhodococcus sp. strain T104 by two different steps of hydrophobic interaction chromatography
to homogeneity, 2 chromatography steps
-
to homogeneity, 2 isoforms, E1, E2
-
to homogeneity, 3step chromatography
-
to homogeneity, chromatography steps
to homogeneity, single chromatography step
to homogeneity, single step chromatography
-
to homogeneity, two chromatograpphy steps
-
two chromatography steps, almost pure as determined by SDS-PAGE
using Ni-NTA chromatography
wild-type and mutant enzyme L441H