Cloned (Comment) | Organism |
---|---|
expressed as a C-terminal His6-tagged fusion in Pichia pastoris | Thermobifida sp. BCC23166 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
p-nitrophenyl butyrate | - |
Thermobifida sp. BCC23166 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45640 | - |
unglycosylated form of PhaZ-Th, SDS-PAGE | Thermobifida sp. BCC23166 |
61000 | - |
expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE | Thermobifida sp. BCC23166 |
70000 | - |
expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE | Thermobifida sp. BCC23166 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida sp. BCC23166 | B3VS85 | fragment | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE | Thermobifida sp. BCC23166 |
Purification (Comment) | Organism |
---|---|
recombinant enzyme with a C-terminal His6 tag, Ni-Sepharose Fast Flow column, SDS-PAGE | Thermobifida sp. BCC23166 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | - |
Thermobifida sp. BCC23166 | 4-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Thermobifida sp. BCC23166 | 4-nitrophenol + butyrate | - |
? | |
p-nitrophenyl myristate + H2O | - |
Thermobifida sp. BCC23166 | 4-nitrophenol + myristate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Thermobifida sp. BCC23166 | 4-nitrophenol + palmitate | - |
? | |
p-nitrophenyl valerate + H2O | - |
Thermobifida sp. BCC23166 | 4-nitrophenol + pentanoate | - |
? | |
poly-[(R)-3-hydroxybutyrate] + H2O | degradation of poly-[(R)-3-hydroxybutyrate] (PHB) films | Thermobifida sp. BCC23166 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhaZ-Th | - |
Thermobifida sp. BCC23166 |
polyhydroxyalkanoate depolymerase | - |
Thermobifida sp. BCC23166 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | substrate p-nitrophenyl butyrate with optimal activity at 50-55°C and pH 7-8 | Thermobifida sp. BCC23166 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no significant difference in catalytic activity and thermostability is observed for the deglycosylated PhaZ-Th compared to the glycosylated form | Thermobifida sp. BCC23166 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | substrate p-nitrophenyl butyrate with optimal activity at 50-55°C and pH 7-8 | Thermobifida sp. BCC23166 |