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3.1.1.75: poly(3-hydroxybutyrate) depolymerase

This is an abbreviated version!
For detailed information about poly(3-hydroxybutyrate) depolymerase, go to the full flat file.

Word Map on EC 3.1.1.75

Reaction

[(R)-3-hydroxybutanoate]n
+
H2O
=
[(R)-3-hydroxybutanoate]n-x
 +
[(R)-3-hydroxybutanoate]x

Synonyms

AFUA_4G03560, i-PHA depolymerase, intracellular poly(3-hydroxybutyrate) depolymerase, intracellular poly(3-hydroybutyrate) depolymerase, MCL-PHA depolymerase, medium chain length polyhydroxyalkanoate depolymerase, P(3HB) depolymerase, P(3HB) depolymerase A, P(3HB) depolymerase B, P(3HB-co-3HV) depolymerase, P(3HV) depolymerase, PDI, PHA depolymerase, PHAase I, PHAase II, PhaZ, PhaZ-Th, PhaZ1, PhaZ1-Z6, phaZ3, PhaZ5, PhaZ6, PhaZ7, PhaZ7 depolymerase, PhaZa1, PhaZBm, PhaZd, PhaZd1, PhaZd2, PhaZRpiT1, PhaZSa, PHB depolymerase, PHB depolymerase A, PHB depolymerase inhibitor, PHB-depolymerase, PHBDP, PHV depolymerase, poly (beta-hydroxybutyrate) depolymerase, poly(3-hydroxybutyrate) (PHB) depolymerase, poly(3-hydroxybutyrate) depolymerase, poly(3-hydroxyvalerate) depolymerase, poly(3-hydroxyvaleric acid) depolymerase, poly(3HB) depolymerase, poly(beta-hydroxybutyrate) depolymerase, poly(HA SCL) depolymerase, poly(HA) depolymerase, poly(hydroxybutyric) depolymerase, poly-beta-hydroxybutyrate depolymerase, poly-beta-hydroxybutyric acid depolymerase, polyhydroxyalkanoate depolymerase, polyhydroxybutyrate depolymerase, poly[(R)-3-hydroxybutyrate] depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly[D(-)-3-hydroxybutyrate] depolymerase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.75 poly(3-hydroxybutyrate) depolymerase

pH Range

pH Range on EC 3.1.1.75 - poly(3-hydroxybutyrate) depolymerase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
-
activity range
4 - 6.5
-
pH 4.0: about 60% of maximal activity, pH 6.5: about 40% of maximal activity
5 - 10
-
pH 5.0: about 40% of maximal activity, pH 10.0: about 30% of maximal activity
6 - 11
-
activity range
6 - 7
-
more than 95% of maximal activity
6 - 8
-
pH 6.0: about 45% of maximal activity, pH 8.0: about 65% of maximal activity
6 - 9.5
-
pH 6.0: about 75% of maximal activity, pH 9.5: about 60% of maximal activity
7 - 12
-
the enzyme is completely inactivated at pH values below pH 7, but shows optimum activity at pH 12
8 - 10
pH 8.0: 70% of maximal activity, pH 10.0: about 85% of maximal activity
8 - 10.5
-
60% of maximal activity within this range