Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.1.75: poly(3-hydroxybutyrate) depolymerase

This is an abbreviated version!
For detailed information about poly(3-hydroxybutyrate) depolymerase, go to the full flat file.

Word Map on EC 3.1.1.75

Reaction

[(R)-3-hydroxybutanoate]n
+
H2O
=
[(R)-3-hydroxybutanoate]n-x
 +
[(R)-3-hydroxybutanoate]x

Synonyms

AFUA_4G03560, i-PHA depolymerase, intracellular poly(3-hydroxybutyrate) depolymerase, intracellular poly(3-hydroybutyrate) depolymerase, MCL-PHA depolymerase, medium chain length polyhydroxyalkanoate depolymerase, P(3HB) depolymerase, P(3HB) depolymerase A, P(3HB) depolymerase B, P(3HB-co-3HV) depolymerase, P(3HV) depolymerase, PDI, PHA depolymerase, PHAase I, PHAase II, PhaZ, PhaZ-Th, PhaZ1, PhaZ1-Z6, phaZ3, PhaZ5, PhaZ6, PhaZ7, PhaZ7 depolymerase, PhaZa1, PhaZBm, PhaZd, PhaZd1, PhaZd2, PhaZRpiT1, PhaZSa, PHB depolymerase, PHB depolymerase A, PHB depolymerase inhibitor, PHB-depolymerase, PHBDP, PHV depolymerase, poly (beta-hydroxybutyrate) depolymerase, poly(3-hydroxybutyrate) (PHB) depolymerase, poly(3-hydroxybutyrate) depolymerase, poly(3-hydroxyvalerate) depolymerase, poly(3-hydroxyvaleric acid) depolymerase, poly(3HB) depolymerase, poly(beta-hydroxybutyrate) depolymerase, poly(HA SCL) depolymerase, poly(HA) depolymerase, poly(hydroxybutyric) depolymerase, poly-beta-hydroxybutyrate depolymerase, poly-beta-hydroxybutyric acid depolymerase, polyhydroxyalkanoate depolymerase, polyhydroxybutyrate depolymerase, poly[(R)-3-hydroxybutyrate] depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly[D(-)-3-hydroxybutyrate] depolymerase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.75 poly(3-hydroxybutyrate) depolymerase

Molecular Weight

Molecular Weight on EC 3.1.1.75 - poly(3-hydroxybutyrate) depolymerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
104000
-
gel filtration
15100
-
x * 15100, SDS-PAGE
28000
-
native PAGE
33800
-
x * 33800, SDS-PAGE
35247
-
1 * 35247, SDS-PAGE
35250
-
MALDI-TOF
35740
calculated from cDNA
37000
38000
His-tagged fusion protein, SDS-PAGE
38400
x * 38400, about, sequence calculation
39168
-
1 * 39168, SDS-PAGE
39170
-
deduced from sequence, SDS-PAGE
39200
x * 39200, about, sequence calculation
41000
-
1 * 41000, SDS-PAGE
42000
42700
-
gel filtration
43000
-
gel filtration
44000
-
isofrom E2, gel filtration
44800
-
x * 44800, SDS-PAGE
45000
45640
unglycosylated form of PhaZ-Th, SDS-PAGE
46000
-
isoform E1, gel filtration
46500
-
deduced from sequence, SDS-PAGE
46800
-
SDS-PAGE
47000
-
native PAGE, SDS-PAGE
48000
1 * 48400, about mass spectrometry, 1 * 48000, SDS-PAGE
48400
1 * 48400, about mass spectrometry, 1 * 48000, SDS-PAGE
49356
1 * 49356, SDS-PAGE
49360
deduced from sequence, SDS-PAGE
53000
-
sucrose density gradient centrifugation
59400
-
x * 59400, SDS-PAGE
60800
-
Pseudomonas putida KTMQ01, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD)
61000
expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE
62000
-
x * 62000, about, SDS-PAGE and mass spectrometry
63744
-
x * 63744, SDS-PAGE
68910
-
Pseudomonas putida KT2442, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD)
70000
expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE
95500
-
native PAGE, SDS-PAGE