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Literature summary for 3.1.1.75 extracted from

  • Takeda, M.; Koizumi, J.I.; Yabe, K.; Adachi, K.
    Thermostable poly(3-hydroxybutyrate) depolymerase of a thermophilic strain of Leptothrix sp. isolated from a hot spring (1998), J. Ferment. Bioeng., 85, 375-380.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
 Leptothrix sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(3-hydroxybutanoate)n 0.018 mg/ml Leptothrix sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Leptothrix sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 gel filtration Leptothrix sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Leptothrix sp. (in: Bacteria)
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Leptothrix sp. (in: Bacteria) HS
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Leptothrix sp. (in: Bacteria)
-
-
-
Leptothrix sp. (in: Bacteria) HS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, single step chromatography Leptothrix sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Leptothrix sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyric acid) + H2O
-
 Leptothrix sp. (in: Bacteria) ?
-
 ?
poly(3-hydroxybutyric acid) + H2O
-
 Leptothrix sp. (in: Bacteria) HS ?
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Leptothrix sp. (in: Bacteria) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Leptothrix sp. (in: Bacteria) HS poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 104000, SDS-PAGE Leptothrix sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Leptothrix sp. (in: Bacteria)