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Literature summary for 3.1.1.75 extracted from
- Properties of an intracellular poly(3-hydroxybutyrate) depolymerase (PhaZ1) from Rhodobacter spheroides (2004), Curr. Microbiol., 49, 199-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cereibacter sphaeroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C178A | no degradation of amorphous poly(3-hydroxybutyrate) granules or 3-hydroxybutyrate oligomers | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoic acid | complete inhibition at 0.1 mM | Cereibacter sphaeroides |  |
| Triton X-100 | complete inhibition at at 0.1% (w/v) | Cereibacter sphaeroides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Cereibacter sphaeroides | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
deduced from sequence, SDS-PAGE | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxybutanoate]n + H2O | Cereibacter sphaeroides | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification from inclusion bodies | Cereibacter sphaeroides |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| purification from inclusion bodies | Cereibacter sphaeroides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Cereibacter sphaeroides | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxybutanoate]n + H2O | - |
Cereibacter sphaeroides | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
| poly[(R)-3-hydroxybutanoate]n + H2O | degradation of amorphous poly(3-hydroxybutyrate), 3-hydroxybutyrate tetramer and pentamer, but not crystalline poly(3-hydroxybutyrate) | Cereibacter sphaeroides | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46500, SDS-PAGE | Cereibacter sphaeroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| intracellular poly(3-hydroybutyrate) depolymerase | - |
Cereibacter sphaeroides |
| PhaZ1 | - |
Cereibacter sphaeroides |
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Release 2023.1 (January 2023)
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