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Literature summary for 3.1.1.75 extracted from

  • Handrick, R.; Reinhardt, S.; Kimmig, P.; Jendrossek, D.
    The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases (2004), J. Bacteriol., 186, 7243-7253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.75

Activating Compound

Activating Compound Comment Organism Structure
ApdA without only reduced hydrolysis varying between 5 to 20% of the rate with activation Rhodospirillum rubrum
Trypsin without only reduced hydrolysis varying between 5 to 20% of the rate with activation Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol strong inhibition at 4 mM Rhodospirillum rubrum
diisopropylfluorophosphate almost complete inhibition at 1 mM Rhodospirillum rubrum
dithiothreitol strong inhibition at 4 mM Rhodospirillum rubrum
EDTA 79% activity at 1 mM, 20% activity at 20 mM Rhodospirillum rubrum
SDS almost complete inhibition at 0.1% Rhodospirillum rubrum
Triton X-100 almost complete inhibition at 0.1% Rhodospirillum rubrum
Tween 20 almost complete inhibition at 0.1% Rhodospirillum rubrum
Tween 80 almost complete inhibition at 0.1% Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Rhodospirillum rubrum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 increase of activity to 130% at 1 mM, but decrease to 75% at 5 mM and to 40% at 10 mM Rhodospirillum rubrum
MgCl2 increase of activity to 140% at 1 mM, but decrease to 75% at 5 mM and to 35% at 10 mM Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35247
-
 1 * 35247, SDS-PAGE Rhodospirillum rubrum
35250
-
 MALDI-TOF Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Rhodospirillum rubrum
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, two chromatograpphy steps Rhodospirillum rubrum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Rhodospirillum rubrum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
74
-
 74000 U/mg, 1 U is defined as the release of 1 nmol p-nitrophenol from p-nitrophenyl phosphate per minute Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Rhodospirillum rubrum poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O highly specific for oligomers or polymers of 3-hydroxybutyrate in the (R) configuration Rhodospirillum rubrum poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35247, SDS-PAGE Rhodospirillum rubrum

Synonyms

Synonyms Comment Organism
PhaZ1
-
 Rhodospirillum rubrum
PHB depolymerase
-
 Rhodospirillum rubrum
poly(3-hydroxybutyrate) depolymerase
-
 Rhodospirillum rubrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodospirillum rubrum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 above rapid loss of activity, at room temperature 50% of activity after 12 h Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Rhodospirillum rubrum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 (25R)-3beta-hydroxycholest-5-en-27-oate diisopropylfluorophosphate Rhodospirillum rubrum

pI Value

Organism Comment pI Value Maximum pI Value
Rhodospirillum rubrum isoelectric focusing
-
 9.5