Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.75 extracted from

  • Mao, H.; Jiang, H.; Su, T.; Wang, Z.
    Purification and characterization of two extracellular polyhydroxyalkanoate depolymerases from Pseudomonas mendocina (2013), Biotechnol. Lett., 35, 1919-1924.
    View publication on PubMed
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Pseudomonas mendocina
EDTA
-
 Pseudomonas mendocina
Fe2+
-
 Pseudomonas mendocina
H2O2
-
 Pseudomonas mendocina
Mn2+
-
 Pseudomonas mendocina
additional information no or poor inhibition by EDTA, Triton X-10, Tween-20, and urea; no or poor inhibition by Triton X-10, Tween-20, and urea Pseudomonas mendocina

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas mendocina
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ slight activation Pseudomonas mendocina
additional information no or poor effects by Na+, K+, Zn2+, Mg2+, Ca2+, and Co2+ Pseudomonas mendocina
additional information no or poor effects by Zn2+, Mg2+, Ca2+, and Co2+ Pseudomonas mendocina
Na+ slight activation Pseudomonas mendocina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33800
-
 x * 33800, SDS-PAGE Pseudomonas mendocina
59400
-
 x * 59400, SDS-PAGE Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina
-
-
-
Pseudomonas mendocina DS04-T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extracellular native enzyme from culture supernatant 2.6fold Pseudomonas mendocina
extracellular native enzyme from culture supernatant 5.6fold Pseudomonas mendocina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is also active on poly(epsilon-caprolactone), cf. EC 3.1.1.76, but not on poly(butylene succinate) and poly(lactic acid) Pseudomonas mendocina ?
-
 ?
additional information the enzyme is also active on poly(epsilon-caprolactone), cf. EC 3.1.1.76, but not on poly(butylene succinate) and poly(lactic acid) Pseudomonas mendocina DS04-T ?
-
 ?
poly(3-hydroxybutyrate) + H2O
-
 Pseudomonas mendocina ?
-
 ?
poly(3-hydroxybutyrate) + H2O
-
 Pseudomonas mendocina DS04-T ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
 Pseudomonas mendocina ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
 Pseudomonas mendocina DS04-T ?
-
 ?
poly(3-hydroxybutyrate-co-4-hydroxybutyrate) + H2O
-
 Pseudomonas mendocina ?
-
 ?
poly(3-hydroxybutyrate-co-4-hydroxybutyrate) + H2O
-
 Pseudomonas mendocina DS04-T ?
-
 ?
poly(lactic acid) + H2O
-
 Pseudomonas mendocina ?
-
 ?
poly(lactic acid) + H2O
-
 Pseudomonas mendocina DS04-T ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33800, SDS-PAGE Pseudomonas mendocina
? x * 59400, SDS-PAGE Pseudomonas mendocina

Synonyms

Synonyms Comment Organism
PHAase I
-
 Pseudomonas mendocina
PHAase II
-
 Pseudomonas mendocina
polyhydroxyalkanoate depolymerase
-
 Pseudomonas mendocina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas mendocina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75 activity range Pseudomonas mendocina
20 85 activity range Pseudomonas mendocina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas mendocina
8.5
-
-
 Pseudomonas mendocina

pH Range

pH Minimum pH Maximum Comment Organism
6 11 activity range Pseudomonas mendocina