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Literature summary for 3.1.1.75 extracted from
- An extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8 (2009), Appl. Microbiol. Biotechnol., 83, 659-668.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 10% (v/v): 102% activity compared to no addition | Thermus thermophilus HB8 |  |
| ethanol | 10% (v/v): 100% activity compared to no addition | Thermus thermophilus HB8 | |
| methanol | 10% (v/v): 137% activity compared to no addition | Thermus thermophilus HB8 | |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | extracellular polyhydroxybutyrate depolymerase is an excellent thermostable candidate biocatalyst for environmental, industrial, and medical applications | Thermus thermophilus HB8 |
| medicine | extracellular polyhydroxybutyrate depolymerase is an excellent thermostable candidate biocatalyst for environmental, industrial, and medical applications | Thermus thermophilus HB8 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1% (v/v): 20% activity, compared to no addition | Thermus thermophilus HB8 | |
| EDTA | 0.1 mM: 51%, 1 mM: 24%, 10 mM: 17% activity, compared to no addition | Thermus thermophilus HB8 | |
| NaCl | 1 mM: 100%, 2 mM: 90%, 5 mM: 60%, 10 mM: 45% activity, compared to no addition | Thermus thermophilus HB8 | |
| NaN3 | 1 mM: 86%, 10 mM: 84% activity, compared to no addition | Thermus thermophilus HB8 | |
| PMSF | 1 mM: 24%, 10 mM: 10% activity, compared to no addition | Thermus thermophilus HB8 | |
| Triton X-100 | 0.1% (v/v): 115%, 1% (v/v): 70%, 10% (v/v): 56% activity, compared to no addition | Thermus thermophilus HB8 | |
| Tween 20 | 0.1% (v/v): 100%, 1% (v/v): 64%, 10% (v/v): 25% activity, compared to no addition | Thermus thermophilus HB8 | |
| Tween 80 | 0.1% (v/v): 80%, 1% (v/v): 65%, 10% (v/v): 42% activity, compared to no addition | Thermus thermophilus HB8 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | apparent Km value of the purified enzyme for polyhydroxybutyrate is 53 microg/ml | Thermus thermophilus HB8 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermus thermophilus HB8 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM: 150%, 2 mM: 206%, 5 mM: 145%, 10 mM: 90% activity | Thermus thermophilus HB8 | |
| KCl | 1 mM: 140%, 2 mM: 150%, 5 mM: 100%, 10 mM: 80% activity | Thermus thermophilus HB8 | |
| MgCl2 | 1 mM: 120%, 2 mM: 90%, 5 mM: 55%, 10 mM: 30% activity | Thermus thermophilus HB8 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
- |
Thermus thermophilus HB8 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus HB8 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Thermus thermophilus HB8 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56.3 | - |
purification step: affinity binding to polyhydroxybutyrate | Thermus thermophilus HB8 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Thermus thermophilus HB8 | 4-nitrophenol + butyrate | - |
? | |
| additional information | purified enzyme is able to hydrolyze 4-nitrophenyl butyrate but not other artificial p-nitrophenyl fatty acid esters, such as p-nitrophenyl octanoate, p-nitrophenyl laurate, indicating preference of this enzyme for C4 substrates | Thermus thermophilus HB8 | ? | - |
? | |
| polyhydroxybutyrate + H2O | - |
Thermus thermophilus HB8 | 3-hydroxybutyrate + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHB depolymerase | - |
Thermus thermophilus HB8 |
| polyhydroxybutyrate depolymerase | - |
Thermus thermophilus HB8 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
optimum temperature of the polyhydroxybutyrate depolymerase enzymic reaction, using polyhydroxybutyrate as substrate, is 70°C | Thermus thermophilus HB8 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at high temperatures | Thermus thermophilus HB8 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermus thermophilus HB8 |
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