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Literature summary for 3.1.1.75 extracted from
- Properties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122 (1995), Appl. Environ. Microbiol., 61, 1727-1730.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| seawater | highest activity by use of 30-100% seawater | Alcaligenes faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba(CH3COO)2 | complete inhibition at 10 mM | Alcaligenes faecalis |  |
| PMSF | complete inactivation at 1 mM | Alcaligenes faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
poly(3-hydroxybutanoate)n | 0.27mg/ml, in 200 mM NaCl | Alcaligenes faecalis | |
| additional information | - |
poly(3-hydroxybutanoate)n | values in mg/ml | Alcaligenes faecalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alcaligenes faecalis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | stimulation due to CaCl2 in seawater | Alcaligenes faecalis | |
| KCl | 200 mM required for maximal stimulation | Alcaligenes faecalis | |
| MgCl2 | stimulation due to MgCl2 in seawater | Alcaligenes faecalis | |
| Na2SO4 | 200 mM required for maximal stimulation | Alcaligenes faecalis | |
| NaCl | stimulation due to NaCl in seawater | Alcaligenes faecalis | |
| SrCl2 | 10 mM required for maximal stimulation | Alcaligenes faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95500 | - |
native PAGE, SDS-PAGE | Alcaligenes faecalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxybutanoate]n + H2O | Alcaligenes faecalis | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | main products: dimer and trimer | ? | |
| poly[(R)-3-hydroxybutanoate]n + H2O | Alcaligenes faecalis AE122 | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | main products: dimer and trimer | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes faecalis | - |
- |
- |
| Alcaligenes faecalis AE122 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 3step chromatography | Alcaligenes faecalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Alcaligenes faecalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
30 mg poly[(R)-3-hydroxybutanoate] /min * mg protein | Alcaligenes faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n + H2O | - |
Alcaligenes faecalis | poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n | - |
? | |
| poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n + H2O | - |
Alcaligenes faecalis AE122 | poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[12%]3-hydroxyvalerate)n | - |
? | |
| poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n + H2O | - |
Alcaligenes faecalis | poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n | - |
? | |
| poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n + H2O | - |
Alcaligenes faecalis AE122 | poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[9%]3-hydroxyvalerate)n | - |
? | |
| poly(3-hydroxybutyric acid) + H2O | - |
Alcaligenes faecalis | ? | - |
? | |
| poly(3-hydroxybutyric acid) + H2O | - |
Alcaligenes faecalis AE122 | ? | - |
? | |
| poly[(R)-3-hydroxybutanoate]n + H2O | - |
Alcaligenes faecalis | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | main products: dimer and trimer | ? | |
| poly[(R)-3-hydroxybutanoate]n + H2O | - |
Alcaligenes faecalis AE122 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | main products: dimer and trimer | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 95500, SDS-PAGE | Alcaligenes faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Alcaligenes faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Alcaligenes faecalis |
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