3.1.1.75: poly(3-hydroxybutyrate) depolymerase
This is an abbreviated version!
For detailed information about poly(3-hydroxybutyrate) depolymerase, go to the full flat file.
Word Map on EC 3.1.1.75
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3.1.1.75
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ralstonia
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alcaligenes
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polyester
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eutropha
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polyr-3-hydroxybutyrate
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medium-chain-length
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lemoignei
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pickettii
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mcl-phas
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poly3-hydroxyalkanoate
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poly3-hydroxybutyrate-co-3-hydroxyvalerate
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phb-degrading
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paucimonas
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phasins
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copolyesters
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polyl-lactide
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oleovorans
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p3hb-co-4hb
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short-chain-length
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degradation
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d--3-hydroxybutyrate
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analysis
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synthesis
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medicine
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industry
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biotechnology
- 3.1.1.75
- ralstonia
- alcaligenes
-
polyester
- eutropha
-
polyr-3-hydroxybutyrate
-
medium-chain-length
- lemoignei
- pickettii
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mcl-phas
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poly3-hydroxyalkanoate
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poly3-hydroxybutyrate-co-3-hydroxyvalerate
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phb-degrading
- paucimonas
-
phasins
-
copolyesters
-
polyl-lactide
- oleovorans
-
p3hb-co-4hb
-
short-chain-length
- degradation
-
d--3-hydroxybutyrate
- analysis
- synthesis
- medicine
- industry
- biotechnology
Reaction
Synonyms
AFUA_4G03560, i-PHA depolymerase, intracellular poly(3-hydroxybutyrate) depolymerase, intracellular poly(3-hydroybutyrate) depolymerase, MCL-PHA depolymerase, medium chain length polyhydroxyalkanoate depolymerase, P(3HB) depolymerase, P(3HB) depolymerase A, P(3HB) depolymerase B, P(3HB-co-3HV) depolymerase, P(3HV) depolymerase, PDI, PHA depolymerase, PHAase I, PHAase II, PhaZ, PhaZ-Th, PhaZ1, PhaZ1-Z6, phaZ3, PhaZ5, PhaZ6, PhaZ7, PhaZ7 depolymerase, PhaZa1, PhaZBm, PhaZd, PhaZd1, PhaZd2, PhaZRpiT1, PhaZSa, PHB depolymerase, PHB depolymerase A, PHB depolymerase inhibitor, PHB-depolymerase, PHBDP, PHV depolymerase, poly (beta-hydroxybutyrate) depolymerase, poly(3-hydroxybutyrate) (PHB) depolymerase, poly(3-hydroxybutyrate) depolymerase, poly(3-hydroxyvalerate) depolymerase, poly(3-hydroxyvaleric acid) depolymerase, poly(3HB) depolymerase, poly(beta-hydroxybutyrate) depolymerase, poly(HA SCL) depolymerase, poly(HA) depolymerase, poly(hydroxybutyric) depolymerase, poly-beta-hydroxybutyrate depolymerase, poly-beta-hydroxybutyric acid depolymerase, polyhydroxyalkanoate depolymerase, polyhydroxybutyrate depolymerase, poly[(R)-3-hydroxybutyrate] depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly[D(-)-3-hydroxybutyrate] depolymerase
ECTree
3.1.1.75 poly(3-hydroxybutyrate) depolymeraseAdvanced search results
results (
results (KM Value
KM Value on EC 3.1.1.75 - poly(3-hydroxybutyrate) depolymerase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.24
4-nitrophenyl acetate
recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C
0.28
4-nitrophenyl acetate
recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C
0.41
4-nitrophenyl acetate
recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C
0.56
4-nitrophenyl acetate
recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C
0.56
4-nitrophenyl acetate
recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C
0.66
4-nitrophenyl acetate
recombinant mutant T37A/N285D, pH 7.0, 30°C
0.67
4-nitrophenyl acetate
recombinant mutant T37A/N285Y, pH 7.0, 30°C
0.73
4-nitrophenyl acetate
recombinant wild-type enzyme, pH 7.0, 30°C
0.82
4-nitrophenyl acetate
recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C
1.96
4-nitrophenyl acetate
recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C
2.43
4-nitrophenyl acetate
recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C
0.16
4-nitrophenyl butyrate
recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C
0.16
4-nitrophenyl butyrate
recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C
0.19
4-nitrophenyl butyrate
recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C
0.23
4-nitrophenyl butyrate
recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C
0.37
4-nitrophenyl butyrate
recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C
0.46
4-nitrophenyl butyrate
recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C
0.51
4-nitrophenyl butyrate
recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C
0.56
4-nitrophenyl butyrate
recombinant wild-type enzyme, pH 7.0, 30°C
0.63
4-nitrophenyl butyrate
recombinant mutant N285D, pH 7.0, 30°C
0.63
4-nitrophenyl butyrate
recombinant mutant T37A/N285Y, pH 7.0, 30°C
0.67
4-nitrophenyl butyrate
recombinant mutant T37A/N285D, pH 7.0, 30°C
0.78
4-nitrophenyl butyrate
recombinant mutant N285Y, pH 7.0, 30°C
1.25
4-nitrophenyl butyrate
recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C
0.05
4-nitrophenyl hexanoate
recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C
0.05
4-nitrophenyl hexanoate
recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C
0.06
4-nitrophenyl hexanoate
recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C
0.06
4-nitrophenyl hexanoate
recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C
0.06
4-nitrophenyl hexanoate
recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C
0.07
4-nitrophenyl hexanoate
recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C
0.09
4-nitrophenyl hexanoate
recombinant mutant A66V, pH 7.0, 30°C
0.1
4-nitrophenyl hexanoate
recombinant wild-type enzyme, pH 7.0, 30°C
0.29
4-nitrophenyl hexanoate
recombinant mutant T37A/N285Y, pH 7.0, 30°C
0.3
4-nitrophenyl hexanoate
recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C
0.32
4-nitrophenyl hexanoate
recombinant mutant N285Y, pH 7.0, 30°C
0.41
4-nitrophenyl hexanoate
recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C
0.43
4-nitrophenyl hexanoate
recombinant mutant T37A/N285D, pH 7.0, 30°C
0.45
4-nitrophenyl hexanoate
recombinant mutant N285D, pH 7.0, 30°C
0.05
4-nitrophenyl pentanoate
recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C
0.09
4-nitrophenyl pentanoate
recombinant mutant A66V, pH 7.0, 30°C
0.09
4-nitrophenyl pentanoate
recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C
0.09
4-nitrophenyl pentanoate
recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C
0.09
4-nitrophenyl pentanoate
recombinant wild-type enzyme, pH 7.0, 30°C
0.1
4-nitrophenyl pentanoate
recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C
0.16
4-nitrophenyl pentanoate
recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C
0.24
4-nitrophenyl pentanoate
recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C
0.46
4-nitrophenyl pentanoate
recombinant mutant T37A/N285Y, pH 7.0, 30°C
0.58
4-nitrophenyl pentanoate
recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C
0.59
4-nitrophenyl pentanoate
recombinant mutant N285Y, pH 7.0, 30°C
0.66
4-nitrophenyl pentanoate
recombinant mutant T37A/N285D, pH 7.0, 30°C
0.71
4-nitrophenyl pentanoate
recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C
0.89
4-nitrophenyl pentanoate
recombinant mutant N285D, pH 7.0, 30°C
0.27
4-nitrophenyl propionate
recombinant wild-type enzyme, pH 7.0, 30°C
0.31
4-nitrophenyl propionate
recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C
0.38
4-nitrophenyl propionate
recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C
0.38
4-nitrophenyl propionate
recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C
0.46
4-nitrophenyl propionate
recombinant mutant A66V, pH 7.0, 30°C
0.53
4-nitrophenyl propionate
recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C
0.54
4-nitrophenyl propionate
recombinant mutant T37A/N285Y, pH 7.0, 30°C
0.68
4-nitrophenyl propionate
recombinant mutant N285D, pH 7.0, 30°C
1.01
4-nitrophenyl propionate
recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C
1.07
4-nitrophenyl propionate
recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C
1.17
4-nitrophenyl propionate
recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C
1.28
4-nitrophenyl propionate
recombinant mutant N285Y, pH 7.0, 30°C
1.29
4-nitrophenyl propionate
recombinant mutant T37A/N285D, pH 7.0, 30°C
2.7
4-nitrophenyl propionate
recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C
additional information
additional information
kinetics of wild-type and mutant enzymes, overview
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additional information
additional information
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apparent Km value of the purified enzyme for polyhydroxybutyrate is 53 microg/ml
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additional information
additional information
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for poly (3-hydroxybutyrate), 0.69 g/l
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additional information
additional information
kinetics of random mutants, overview
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additional information
poly(2-hydroxybutanoate)
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Km value 1.04 microg/ml, pH 5.0, 50°C
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additional information
poly(3-hydroxybutanoate)n
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0.27mg/ml, in 200 mM NaCl
additional information
poly(3-hydroxybutanoate)n
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0.018 mg/ml, isoform E1, 0.07 mg/ml, isoform E2
additional information
poly(3-hydroxybutanoate)n
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0.06 mg/ml, P(3HB) depolymerase B, 0.1 mg/ml, P(3HB) depolymerase A
