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Literature summary for 3.1.1.75 extracted from
- Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442 (2009), Biores. Technol., 100, 2265-2270.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant plasmids are introduced into Escherichia coli S17-1 by electroporation. Subsequently, they are introduced into Pseudomonas putida KT2442 via conjugation | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| KTMQ01 | phaZ knockout mutant, Pseudomonas putida KTMQ01 | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60800 | - |
Pseudomonas putida KTMQ01, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD) | Pseudomonas putida |
| 68910 | - |
Pseudomonas putida KT2442, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD) | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
KT2442 and KTMQ01 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyhydroxyalkanoate + H2O | - |
Pseudomonas putida | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHA depolymerase | - |
Pseudomonas putida |
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