3.1.1.75: poly(3-hydroxybutyrate) depolymerase
This is an abbreviated version!
For detailed information about poly(3-hydroxybutyrate) depolymerase, go to the full flat file.
Word Map on EC 3.1.1.75
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3.1.1.75
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ralstonia
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alcaligenes
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polyester
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eutropha
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polyr-3-hydroxybutyrate
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medium-chain-length
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lemoignei
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pickettii
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mcl-phas
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poly3-hydroxyalkanoate
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poly3-hydroxybutyrate-co-3-hydroxyvalerate
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phb-degrading
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paucimonas
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phasins
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copolyesters
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polyl-lactide
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oleovorans
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p3hb-co-4hb
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short-chain-length
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degradation
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d--3-hydroxybutyrate
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analysis
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synthesis
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medicine
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industry
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biotechnology
- 3.1.1.75
- ralstonia
- alcaligenes
-
polyester
- eutropha
-
polyr-3-hydroxybutyrate
-
medium-chain-length
- lemoignei
- pickettii
-
mcl-phas
-
poly3-hydroxyalkanoate
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poly3-hydroxybutyrate-co-3-hydroxyvalerate
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phb-degrading
- paucimonas
-
phasins
-
copolyesters
-
polyl-lactide
- oleovorans
-
p3hb-co-4hb
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short-chain-length
- degradation
-
d--3-hydroxybutyrate
- analysis
- synthesis
- medicine
- industry
- biotechnology
Reaction
Synonyms
AFUA_4G03560, i-PHA depolymerase, intracellular poly(3-hydroxybutyrate) depolymerase, intracellular poly(3-hydroybutyrate) depolymerase, MCL-PHA depolymerase, medium chain length polyhydroxyalkanoate depolymerase, P(3HB) depolymerase, P(3HB) depolymerase A, P(3HB) depolymerase B, P(3HB-co-3HV) depolymerase, P(3HV) depolymerase, PDI, PHA depolymerase, PHAase I, PHAase II, PhaZ, PhaZ-Th, PhaZ1, PhaZ1-Z6, phaZ3, PhaZ5, PhaZ6, PhaZ7, PhaZ7 depolymerase, PhaZa1, PhaZBm, PhaZd, PhaZd1, PhaZd2, PhaZRpiT1, PhaZSa, PHB depolymerase, PHB depolymerase A, PHB depolymerase inhibitor, PHB-depolymerase, PHBDP, PHV depolymerase, poly (beta-hydroxybutyrate) depolymerase, poly(3-hydroxybutyrate) (PHB) depolymerase, poly(3-hydroxybutyrate) depolymerase, poly(3-hydroxyvalerate) depolymerase, poly(3-hydroxyvaleric acid) depolymerase, poly(3HB) depolymerase, poly(beta-hydroxybutyrate) depolymerase, poly(HA SCL) depolymerase, poly(HA) depolymerase, poly(hydroxybutyric) depolymerase, poly-beta-hydroxybutyrate depolymerase, poly-beta-hydroxybutyric acid depolymerase, polyhydroxyalkanoate depolymerase, polyhydroxybutyrate depolymerase, poly[(R)-3-hydroxybutyrate] depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly[D(-)-3-hydroxybutyrate] depolymerase
ECTree
3.1.1.75 poly(3-hydroxybutyrate) depolymeraseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 3.1.1.75 - poly(3-hydroxybutyrate) depolymerase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
1,2-epoxy-3-(p-nitrophenoxy)propane
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complete inactivation at 100 mM, slow inactivation process
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/glycinamide
33% inhibition at 5 mM
dithioerythritol
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complete inhibition of P(3HV) depolymerase at 4 mM, reversible by dithionitrobenzene
Phenylmethylsulfonylfluoride
10% inhibition at 1 mM and 70% inhibition at 10 mM
Ca2+
activates up to 10 mM, inhibits above; activates up to 10 mM, inhibits above
diazo-DL-norleucine methyl ester
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complete inhibition at 25 mM, pseudo-first-order kinetic
diisopropylfluorophosphate
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at 10 microM 53% inhibition, at 100 microM total loss of activity
EDTA
activates alone, but inhibits in presence of divalent cations; activates alone, but inhibits in presence of divalent cations
EDTA
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partial inhibition of P(3HV) depolymerase at concentrations higher than 5 mM
EDTA
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0.1 mM: 51%, 1 mM: 24%, 10 mM: 17% activity, compared to no addition
Hg2+
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90% inhibition at 6 mM, completely reversible by 2-mercaptoethanol
Mg2+
activates up to 10 mM, inhibits above; activates up to 10 mM, inhibits above
NaCl
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required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme
NaCl
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1 mM: 100%, 2 mM: 90%, 5 mM: 60%, 10 mM: 45% activity, compared to no addition
SDS
almost complete inhibition at 0.01% v/v; almost complete inhibition at 0.01% v/v
Triton X-100
almost complete inhibition at 0.01% v/v; almost complete inhibition at 0.01% v/v
Triton X-100
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0.1% (v/v): 115%, 1% (v/v): 70%, 10% (v/v): 56% activity, compared to no addition
Tween 20
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0.1% (v/v): 100%, 1% (v/v): 64%, 10% (v/v): 25% activity, compared to no addition
Tween 80
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0.1% (v/v): 80%, 1% (v/v): 65%, 10% (v/v): 42% activity, compared to no addition
additional information
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no inhibition by phenylmethylsulfonyl fluoride
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additional information
activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenyl butyrate. Poor effect by DTT; activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenylbutyrate. Poor effect by DTT
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additional information
activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenyl butyrate. Poor effect by DTT; activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenylbutyrate. Poor effect by DTT
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additional information
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activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenyl butyrate. Poor effect by DTT; activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenylbutyrate. Poor effect by DTT
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additional information
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no or poor inhibition by EDTA, Triton X-10, Tween-20, and urea; no or poor inhibition by Triton X-10, Tween-20, and urea
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additional information
poor inhibition by glycerol. Increase of ionic strength gradually inhibits the poly(3-hydroxybutyrate) depolymerase activity leading to its complete inactivation at 2.5 M NaCl
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additional information
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poor inhibition by glycerol. Increase of ionic strength gradually inhibits the poly(3-hydroxybutyrate) depolymerase activity leading to its complete inactivation at 2.5 M NaCl
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