Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Paucimonas lemoignei |
Protein Variants | Comment | Organism |
---|---|---|
A134G | PHB depolymerase activity similar to wild type | Paucimonas lemoignei |
A134G, H135L | PHB depolymerase activity similar to wild type, 2fold lipase activity compared to wild type | Paucimonas lemoignei |
D242A | no PHB depolymerase activity | Paucimonas lemoignei |
D242N | no PHB depolymerase activity | Paucimonas lemoignei |
D256A | PHB depolymerase activity similar to wild type | Paucimonas lemoignei |
D256N | PHB depolymerase activity similar to wild type | Paucimonas lemoignei |
H306A | no PHB depolymerase activity | Paucimonas lemoignei |
H47A | no PHB depolymerase activity | Paucimonas lemoignei |
S136A | no PHB depolymerase activity | Paucimonas lemoignei |
S136C | no expression | Paucimonas lemoignei |
S136T | 20% PHB depolymerase activity | Paucimonas lemoignei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paucimonas lemoignei | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly[(R)-3-hydroxybutanoate]n + H2O | Paucimonas lemoignei | specific for amorphous PHB | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paucimonas lemoignei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paucimonas lemoignei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Paucimonas lemoignei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butanoate + H2O | wild type lipase activity is rather low | Paucimonas lemoignei | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | wild type lipase activity is rather low | Paucimonas lemoignei | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | wild type lipase activity is rather low | Paucimonas lemoignei | 4-nitrophenol + octanoate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Paucimonas lemoignei | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl-decanoate + H2O | wild type lipase activity is rather low | Paucimonas lemoignei | p-nitrophenol + decanoate | - |
? | |
p-nitrophenyl-palmitate + H2O | wild type lipase activity is rather low | Paucimonas lemoignei | p-nitrophenol + palmitate | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous PHB | Paucimonas lemoignei | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous poly(3-hydroxybutyrate) | Paucimonas lemoignei | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHA depolymerase | - |
Paucimonas lemoignei |
PhaZ7 | - |
Paucimonas lemoignei |