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3.1.1.23: acylglycerol lipase

This is an abbreviated version!
For detailed information about acylglycerol lipase, go to the full flat file.

Word Map on EC 3.1.1.23

Reaction

CnHmCO-C3O3H7
+
H2O
=
CnHmCO2H
 +
glycerol

Synonyms

AtMAGL, AtMAGL1, AtMAGL10, AtMAGL11, AtMAGL14, AtMAGL15, AtMAGL16, AtMAGL2, AtMAGL3, AtMAGL4, AtMAGL5, AtMAGL6, AtMAGL7, AtMAGL8, AtMAGL9, fatty acyl monoester lipase, glycerol-monoester acylhydrolase, HMGL, LH57_01015, lipase, lipase G, LipB, lipozyme IM, MAG hydrolase, MAG lipase, MAGL, MAGL-cy, MAGL-m, MAGL16, MAGL6, MAGL8, MGH, MGL, MGL Rv0183, MGL-like activity, MGLL, MGLP, monoacylglycerol hydrolase, monoacylglycerol lipase, monoglycerid lipase, monoglyceridase, monoglyceride hydrolase, monoglyceride lipase, monoglyceride lipase-like activity, monoglyceridyllipase, MSMEG_0220, mtbMGL, patatin-like protein 1, patatin-like protein 16, patatin-like protein 2, patatin-like protein 3, patatin-like protein 4, patatin-like protein 5, patatin-like protein 6, patatin-like protein 7, patatin-like protein 8, patatin-like protein 9, PLP1, PLP16, PLP2, PLP3, PLP4, PLP5, PLP6, PLP7, PLP8, PLP9, Rv0183, Rv0183 protein, Yju3p

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.23 acylglycerol lipase

Purification

Purification on EC 3.1.1.23 - acylglycerol lipase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
-
by a combination of streptactin and ion metal affinity chromatography
by gel filtration
-
His-Trap column chromatography and Superdex 200 gel filtration
HisTrap column chromatography, gel filtration
-
immobilized metal affinity chromatography
immobilized metal affinity column chromatography
-
MAGL-His6 protein purified on nickel-nitrilotriacetic acid column, more than 90% pure
-
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography and Superdex 200 gel filtration
-
Ni-Sepharose column chromatography and Superdex 75 gel filtration
-
octyl-Sepharose column chromatography, cation exchange column chromatography, and gel filtration
partially purified
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) CodonPlus by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag
-
recombinant His6-tagged and streptavidin-tagged enzyme from Escherichia coli to homogeneity by Strep-tag targeting afffinity chromatography and nickel affinity chromatography
-
recombinant MBP-tagged wild-type and mutant enzymes from Escherichia coli by amylose affinity chromatography
separation of bovine brain monoacylglycerol lipase and diacylglycerol lipase by heparin Sepharose affinity chromatography
-
Talon metal affinity resin chromatography
-
TALON metal affinity resin column chromatography
-