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Literature summary for 3.1.1.23 extracted from

  • Cotes, K.; Dhouib, R.; Douchet, I.; Chahinian, H.; de Caro, A.; Carriere, F.; Canaan, S.
    Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids (2007), Biochem. J., 408, 417-427.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
taurodeoxycholate dependent on, maximal activity at 1-5 mM Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv0183, DNA and amino acid sequence determination and anaylsis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D226A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
D226N site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
H256A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
S110A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
E600 inactivation Mycobacterium tuberculosis
additional information inactivation by serine esterase inhibitors Mycobacterium tuberculosis
PMSF inactivation Mycobacterium tuberculosis
tetrahydrolipstatin inactivation Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Mycobacterium tuberculosis 5618
-
extracellular
-
Mycobacterium tuberculosis
-
-
additional information immunolocalization studies Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30223
-
x * 30223, detagged enzyme, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids ?
-
?
additional information Mycobacterium tuberculosis H37Rv Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
gene Rv0183
-
Mycobacterium tuberculosis H37Rv
-
gene Rv0183
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
CnHmCO-C3O3H7 + H2O = CnHmCO2H + glycerol the catalytic triad is composed of Ser110, Asp226 and His256 residues Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
290
-
purified recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dicaprin + H2O
-
Mycobacterium tuberculosis ?
-
?
1,2-dicaprin + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?
1-mono-oleyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis oleate + glycerol
-
?
1-monobutyroyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis butyrate + glycerol
-
?
1-monodecanoyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis decanoate + glycerol
-
?
1-monolauroyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis laureate + glycerol
-
?
1-monomyristoyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis myristoate + glycerol
-
?
1-monooctanoyl-rac-glycerol + H2O
-
Mycobacterium tuberculosis octanoate + glycerol
-
?
diolein + H2O
-
Mycobacterium tuberculosis ?
-
?
diolein + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?
additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis ?
-
?
additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis ?
-
?
additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis H37Rv ?
-
?
additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis H37Rv ?
-
?
soybean oil + H2O
-
Mycobacterium tuberculosis ?
-
?
soybean oil + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

Subunits Comment Organism
? x * 30223, detagged enzyme, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
monoglyceride lipase
-
Mycobacterium tuberculosis
Rv0183 protein
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 50% of maximal activity at pH 6.5, no activity below pH 6.0, maximal activity at pH 7.5-9.0 Mycobacterium tuberculosis