Activating Compound | Comment | Organism | Structure |
---|---|---|---|
taurodeoxycholate | dependent on, maximal activity at 1-5 mM | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene Rv0183, DNA and amino acid sequence determination and anaylsis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D226A | site-directed mutagenesis, active site residue mutation, the catalytic activity is affected | Mycobacterium tuberculosis |
D226N | site-directed mutagenesis, active site residue mutation, the catalytic activity is affected | Mycobacterium tuberculosis |
H256A | site-directed mutagenesis, active site residue mutation, the catalytic activity is affected | Mycobacterium tuberculosis |
S110A | site-directed mutagenesis, active site residue mutation, the catalytic activity is affected | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E600 | inactivation | Mycobacterium tuberculosis | |
additional information | inactivation by serine esterase inhibitors | Mycobacterium tuberculosis | |
PMSF | inactivation | Mycobacterium tuberculosis | |
tetrahydrolipstatin | inactivation | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Mycobacterium tuberculosis | 5618 | - |
extracellular | - |
Mycobacterium tuberculosis | - |
- |
additional information | immunolocalization studies | Mycobacterium tuberculosis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30223 | - |
x * 30223, detagged enzyme, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv0183 | - |
Mycobacterium tuberculosis H37Rv | - |
gene Rv0183 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CnHmCO-C3O3H7 + H2O = CnHmCO2H + glycerol | the catalytic triad is composed of Ser110, Asp226 and His256 residues | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
290 | - |
purified recombinant enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dicaprin + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
1,2-dicaprin + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
1-mono-oleyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | oleate + glycerol | - |
? | |
1-monobutyroyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | butyrate + glycerol | - |
? | |
1-monodecanoyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | decanoate + glycerol | - |
? | |
1-monolauroyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | laureate + glycerol | - |
? | |
1-monomyristoyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | myristoate + glycerol | - |
? | |
1-monooctanoyl-rac-glycerol + H2O | - |
Mycobacterium tuberculosis | octanoate + glycerol | - |
? | |
diolein + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
diolein + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids | Mycobacterium tuberculosis | ? | - |
? | |
additional information | substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
soybean oil + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
soybean oil + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30223, detagged enzyme, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
monoglyceride lipase | - |
Mycobacterium tuberculosis |
Rv0183 protein | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 50% of maximal activity at pH 6.5, no activity below pH 6.0, maximal activity at pH 7.5-9.0 | Mycobacterium tuberculosis |