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Literature summary for 3.1.1.23 extracted from

  • Somma-Delpero, C.; Valette, A.; Lepetit-Thevenin, J.; Nobili, O.; Boyer, J.; Verine, A.
    Purification and properties of a monoacylglycerol lipase in human erythrocytes (1995), Biochem. J., 312, 519-525.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.23

Inhibitors

Inhibitors Comment Organism Structure
apolipoprotein A-1
-
 Homo sapiens
Diethyl p-nitrophenyl phosphate
-
 Homo sapiens
diisopropyl fluorophosphate
-
 Homo sapiens
NaCl 1 M, 63% loss of activity Homo sapiens
NaF
-
 Homo sapiens
PMSF
-
 Homo sapiens
SDS
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 1(3)-monooleoylglycerol
-
 Homo sapiens
0.47
-
 ethyl oleate
-
 Homo sapiens
0.49
-
 sn-2-monooleoylglycerol
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Homo sapiens
70000
-
 1 * 70000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
pancreatic islet
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, 8 mM CHAPS, 5-7% loss of activity per day Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-lysophosphatidylcholine + H2O
-
 Homo sapiens palmitic acid + glycerophosphorylcholine
-
 ?
2-monoolein + H2O
-
 Homo sapiens ?
-
 ?
ethyl oleate + H2O
-
 Homo sapiens oleic acid + ethanol
-
 ?
monooleoylglycerol + H2O preferred substrate Homo sapiens oleic acid + glycerol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, more than 60% loss of activity Homo sapiens
55
-
 15 min, complete inactivation Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens