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Literature summary for 3.1.1.23 extracted from
- A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase (2000), Eur. J. Biochem., 267, 6459-6469.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A76G | reduced thermostability, increased stability at pH 5-7, substitution converts the esterase into a monoacylglycerol hydrolase | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19490 | - |
calculated from amino acid sequence | Bacillus subtilis |
| 20500 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | Q79F14 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl caprate + H2O | - |
Bacillus subtilis | 4-nitrophenol + capric acid | - |
? |  |
| 4-nitrophenyl octanoate + H2O | - |
Bacillus subtilis | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl-caproate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caproic acid | - |
? | |
| 4-nitrophenyl-laurate + H2O | - |
Bacillus subtilis | 4-nitrophenol + lauric acid | - |
? | |
| 4-nitrophenyl-myristate + H2O | - |
Bacillus subtilis | 4-nitrophenol + myristic acid | - |
? | |
| 4-nitrophenyl-palmitate + H2O | - |
Bacillus subtilis | 4-nitrophenol + palmitic acid | - |
? | |
| 4-nitrophenyl-stearate + H2O | - |
Bacillus subtilis | 4-nitrophenol + stearic acid | - |
? | |
| additional information | preferentially hydrolyzes triacylglycerol-esters and 4-nitrophenyl-esters of fatty acids with short chain lengths up to 10 carbon atoms, no hydrolysis of triolein | Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipB | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
wild-type enzyme is stable, A76G mutant loses 50% activity after 8 min | Bacillus subtilis |
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Release 2023.1 (January 2023)
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