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3.1.1.2: arylesterase

This is an abbreviated version!
For detailed information about arylesterase, go to the full flat file.

Word Map on EC 3.1.1.2

Reaction

a phenyl acetate
+
H2O
=
a phenol
 +
acetate

Synonyms

(R)-specific SGNH arylesterase, A-esterase, alpha-esterase, ARE, AREase, aromatic esterase, Aryl-ester hydrolase, aryldialkylphosphatase, arylesterase, DvvII, EST-1, EST-2, EST-3, ESt-A, Est0881, EstB, EstR5, G3C9 rePON1, gox0881, HDL-PON1, K-45, lp_1002, More, neuropathy target esterase-related esterase, NRE, NRECV, NTE-R1, NTE-related 1, NTE-related esterase, paraoxonase, paraoxonase 1, paraoxonase-1, paraoxonase/arylesterase, paraoxonase1, patatin-like phospholipase 7, phenyl acetate esterase, phenyl valerate esterase, PNPLA7, PON-aryl, PON1, PON1/Aryl, pyrethroid-resistance-associated esterase, Saci_2140, SacPox, serum paraoxonase/arylesterase 1, SGNH arylesterase, SGNH-arylesterase, SisLac, Sm23, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.2 arylesterase

Purification

Purification on EC 3.1.1.2 - arylesterase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms, pI 4.9 form and pI 5.9 form
-
ammonium sulfate precipitation, column chromatography, gel filtration
-
ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration
-
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 75 gel filtration
anion-exchange chromatography and concanavalin-A chromatography
blue agarose affinity chromatography and HiTrap DEAE column chromatography
by different types of chromatographies
-
by immobilized metal-ion affinity chromatography
-
by pseudo-affinity chromatography and gel filtration
-
by sonication and on Ni-NTA affinity resin
-
by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield
-
ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography
co-purification of PON1 and phosphate binding protein from high-density-lipoprotein-particles using hydroxyapatite chromatography, method development
DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration
HisTrap column chromatography
native enzyme from plasma of fasted humans by sequential chromatographic steps
-
Ni+2-NTA resin chromatography
-
partially
purification of serum PON1 from different bovine breeds namely Swiss Black, Holstein, and Montofon
-
Q-Sepharose column chromatography
-
recombinant enzyme
recombinant enzyme is purified by DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli, to homogeneity
the presence of 2.5 mM Ca2+ and 0.1% (w/v) Triton X-100 (as detergent) in the buffers throughout the purification procedure is essential for maintaining the activity of the enzyme. In the absence of calcium and Triton X-100, the enzyme activity is quickly lost
two A-esterases, difficult to separate
-