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3.1.1.2: arylesterase

This is an abbreviated version!
For detailed information about arylesterase, go to the full flat file.

Word Map on EC 3.1.1.2

Reaction

a phenyl acetate
+
H2O
=
a phenol
 +
acetate

Synonyms

(R)-specific SGNH arylesterase, A-esterase, alpha-esterase, ARE, AREase, aromatic esterase, Aryl-ester hydrolase, aryldialkylphosphatase, arylesterase, DvvII, EST-1, EST-2, EST-3, ESt-A, Est0881, EstB, EstR5, G3C9 rePON1, gox0881, HDL-PON1, K-45, lp_1002, More, neuropathy target esterase-related esterase, NRE, NRECV, NTE-R1, NTE-related 1, NTE-related esterase, paraoxonase, paraoxonase 1, paraoxonase-1, paraoxonase/arylesterase, paraoxonase1, patatin-like phospholipase 7, phenyl acetate esterase, phenyl valerate esterase, PNPLA7, PON-aryl, PON1, PON1/Aryl, pyrethroid-resistance-associated esterase, Saci_2140, SacPox, serum paraoxonase/arylesterase 1, SGNH arylesterase, SGNH-arylesterase, SisLac, Sm23, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.2 arylesterase

Cloned

Cloned on EC 3.1.1.2 - arylesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a high level of recombinant hPON1 type A (rPON1A) is produced by Hi-5 insect cells. A fraction is secreted into the cell culture medium, but the majority remains associated with the host insect cells
-
application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis
bacterially expressed chimeric G2E6
-
directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state
expressed from Trichoplusia ni larvae
-
expressed in baculovirus system in Hi5 insect cells
expressed in Drosophila melanogaster
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Gold(DE3) cells
expressed in Escherichia coli XL1-Blue cells
-
expressed in HEK-293 cells
-
expressed in Trichoplusia ni larvae
-
expression in Escherichia coli
expression of PON3 in Spodoptera frugiperda Sf9 cells using the baculovirus expression system
gene estA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as C-terminally HIs-tagged protein
gene estB, DNA and amino acid sequence determination and analysis, expression as His-tagged protein in Escherichia coli
gene PON1, genotyping, naturally occuring polymorphisms in the gene coding and the promoter regions affect the activity and the enzyme level
gene PON1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain Origami B (DE3)
genotyping of naturally occuring polymorphisms, allele frequency determination
heterologously expressed in Escherichia coli
overexpression in Escherichia coli
-
PCR product inserted into pQE30 vector and expressed in Escherichia coli XL1-Blue
-
the recombinant enzyme is expressed in Trichoplusia ni High Five insect cells