Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.1.2: arylesterase

This is an abbreviated version!
For detailed information about arylesterase, go to the full flat file.

Word Map on EC 3.1.1.2

Reaction

a phenyl acetate
+
H2O
=
a phenol
 +
acetate

Synonyms

(R)-specific SGNH arylesterase, A-esterase, alpha-esterase, ARE, AREase, aromatic esterase, Aryl-ester hydrolase, aryldialkylphosphatase, arylesterase, DvvII, EST-1, EST-2, EST-3, ESt-A, Est0881, EstB, EstR5, G3C9 rePON1, gox0881, HDL-PON1, K-45, lp_1002, More, neuropathy target esterase-related esterase, NRE, NRECV, NTE-R1, NTE-related 1, NTE-related esterase, paraoxonase, paraoxonase 1, paraoxonase-1, paraoxonase/arylesterase, paraoxonase1, patatin-like phospholipase 7, phenyl acetate esterase, phenyl valerate esterase, PNPLA7, PON-aryl, PON1, PON1/Aryl, pyrethroid-resistance-associated esterase, Saci_2140, SacPox, serum paraoxonase/arylesterase 1, SGNH arylesterase, SGNH-arylesterase, SisLac, Sm23, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.2 arylesterase

Temperature Stability

Temperature Stability on EC 3.1.1.2 - arylesterase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
boiling completely abolishes activity
102
Tm-value, wild-type enzyme
40 - 55
180 min, quite stable
45 - 60
the enzyme is stable at 45°C, retaining 91.5% of the activity after 1 h incubation. At 60°C, the enzyme activity decreases rapidly and only retains 16.8% of the activity after 1 h incubation
50
-
is resistant to thermal denaturation up to 50°C. Sm23 irreversibly unfolds after heating over 60°C
50 - 60
50 - 94
the enzyme retains 52% of its activity after 50 h of incubation at 90°C, the enzyme is rapidly inactivated above 94°C, most of the enzyme activity is maintained for 5 days at 50°C, the enzyme activity gradually decreases with time at higher temperatures, approximately 70% of the enzyme activity remains after 5 days at 70°C, and 52% of it is still preserved after 50 h at 90°C, however, at 90°C after 5 days, the enzyme is completely inactivated
65
-
no loss of activity after 180 min at 65°C
75
-
about 20% loss of activity after 180 min at 75°C
85
-
about 40% loss of activity after 180 min at 85°C
94
Tm-value, mutant enzyme Y34Q
95
half-life: 3.6 min
96
Tm-value, mutant enzyme E14K
98
Tm-value, mutant enzyme E14K/Y34Q
additional information