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Literature summary for 3.1.1.2 extracted from

  • Inmaculada, N.; Francisco, G.
    Characterization of cross-linked immobilized arylesterase from Gluconobacter oxydans 621H with activity toward cephalosporin C and 7-aminocephalosporanic acid (2016), Biotechnol. Prog., 32, 36-42 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
SDS complete inhibition at 5 and 10% (w/v) Gluconobacter oxydans
Triton X-100 61% residual activity at 10% (v/v) Gluconobacter oxydans
Tween 20 51% residual activity at 10% (v/v) Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.039
-
1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.069
-
phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.072
-
4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.094
-
4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.1
-
2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
0.369
-
1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
7.1
-
S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
74.6
-
7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
98.7
-
7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
119
-
cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
174.7
-
cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require a metal cofactor Gluconobacter oxydans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone in the presence of 40% (v/v) acetone, the cross-linked enzyme aggregate maintains 80% activity while the native enzyme totally loses its activity Gluconobacter oxydans
acetonitrile in the presence of 40% (v/v) acetonitrile, the cross-linked enzyme aggregate maintains 33% activity Gluconobacter oxydans
DMSO in the presence of 40% (v/v) DMSO, the cross-linked enzyme aggregate maintains 73% activity Gluconobacter oxydans
Ethanol with 40% (v/v) ethanol, the cross-linked enzyme aggregate retains 100% activity, whereas the free enzyme loses its activity Gluconobacter oxydans
additional information the addition of organic solvents, such as methanol, ethanol, acetonitrile, acetone, DMSO, and propanol, had little effect on activity when used at 5% and 10% (v/v) Gluconobacter oxydans
propanol in the presence of 40% (v/v) propanol, the cross-linked enzyme aggregate maintains 11% activity Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans 621H
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
Gluconobacter oxydans 1-naphthol + acetate
-
?
1-naphthyl acetate + H2O
-
Gluconobacter oxydans 621H 1-naphthol + acetate
-
?
1-naphthyl butyrate + H2O
-
Gluconobacter oxydans 1-naphthol + butyrate
-
?
2-naphthyl acetate + H2O
-
Gluconobacter oxydans 2-naphthol + acetate
-
?
2-naphthyl acetate + H2O
-
Gluconobacter oxydans 621H 2-naphthol + acetate
-
?
2-naphthyl butyrate + H2O
-
Gluconobacter oxydans 2-naphthol + butyrate
-
?
4-nitrophenyl acetate + H2O
-
Gluconobacter oxydans 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Gluconobacter oxydans 621H 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O best substrate Gluconobacter oxydans 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O best substrate Gluconobacter oxydans 621H 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O
-
Gluconobacter oxydans 4-nitrophenol + caprylate
-
?
4-nitrophenyl laurate + H2O
-
Gluconobacter oxydans 4-nitrophenol + laurate
-
?
7-aminocephalosporanic acid + H2O
-
Gluconobacter oxydans ?
-
?
cephalosporin C + H2O
-
Gluconobacter oxydans ?
-
?
phenyl acetate + H2O
-
Gluconobacter oxydans phenol + acetate
-
?
phenyl acetate + H2O
-
Gluconobacter oxydans 621H phenol + acetate
-
?
S-methyl thiobutanoate + H2O
-
Gluconobacter oxydans methanol + thiobutanoate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
native enzyme Gluconobacter oxydans
50
-
cross-linked enzyme aggregate Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 after 5 min of incubation at 60°C, 20 min at 55°C and 40 min at 50°C, the immobilized cross-linked enzyme aggregate loses all of its initial activity. However, at 40°C, it is still slightly active after 7 h incubation Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
9.3
-
S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
9.8
-
1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
15.2
-
2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
17.6
-
4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
20.81
-
cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
22.7
-
2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
25.7
-
4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
42.7
-
cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
61.3
-
7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
69.5
-
7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
102
-
phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
native enzyme Gluconobacter oxydans
8
-
cross-linked enzyme aggregate Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
cephalosporin C immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
0.9
-
7-aminocephalosporanic acid immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C Gluconobacter oxydans
1
-
S-methyl thiobutanoate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
20
-
1-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
110
-
1-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
220
-
2-naphthyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
240
-
4-nitrophenyl butyrate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
270
-
4-nitrophenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
1100
-
2-naphthyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
1480
-
phenyl acetate immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C Gluconobacter oxydans
2400
-
7-aminocephalosporanic acid free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans
2700
-
cephalosporin C free enzyme, at pH 8.0 and 30°C Gluconobacter oxydans