Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | complete inhibition at 5 and 10% (w/v) | Gluconobacter oxydans | |
Triton X-100 | 61% residual activity at 10% (v/v) | Gluconobacter oxydans | |
Tween 20 | 51% residual activity at 10% (v/v) | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.039 | - |
1-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.069 | - |
phenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.072 | - |
4-nitrophenyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.094 | - |
4-nitrophenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.1 | - |
2-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
0.369 | - |
1-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
7.1 | - |
S-methyl thiobutanoate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
74.6 | - |
7-aminocephalosporanic acid | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans | |
98.7 | - |
7-aminocephalosporanic acid | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans | |
119 | - |
cephalosporin C | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans | |
174.7 | - |
cephalosporin C | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require a metal cofactor | Gluconobacter oxydans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | in the presence of 40% (v/v) acetone, the cross-linked enzyme aggregate maintains 80% activity while the native enzyme totally loses its activity | Gluconobacter oxydans |
acetonitrile | in the presence of 40% (v/v) acetonitrile, the cross-linked enzyme aggregate maintains 33% activity | Gluconobacter oxydans |
DMSO | in the presence of 40% (v/v) DMSO, the cross-linked enzyme aggregate maintains 73% activity | Gluconobacter oxydans |
Ethanol | with 40% (v/v) ethanol, the cross-linked enzyme aggregate retains 100% activity, whereas the free enzyme loses its activity | Gluconobacter oxydans |
additional information | the addition of organic solvents, such as methanol, ethanol, acetonitrile, acetone, DMSO, and propanol, had little effect on activity when used at 5% and 10% (v/v) | Gluconobacter oxydans |
propanol | in the presence of 40% (v/v) propanol, the cross-linked enzyme aggregate maintains 11% activity | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans 621H | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Gluconobacter oxydans | 1-naphthol + acetate | - |
? | |
1-naphthyl acetate + H2O | - |
Gluconobacter oxydans 621H | 1-naphthol + acetate | - |
? | |
1-naphthyl butyrate + H2O | - |
Gluconobacter oxydans | 1-naphthol + butyrate | - |
? | |
2-naphthyl acetate + H2O | - |
Gluconobacter oxydans | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Gluconobacter oxydans 621H | 2-naphthol + acetate | - |
? | |
2-naphthyl butyrate + H2O | - |
Gluconobacter oxydans | 2-naphthol + butyrate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Gluconobacter oxydans | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Gluconobacter oxydans 621H | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | best substrate | Gluconobacter oxydans | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | best substrate | Gluconobacter oxydans 621H | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Gluconobacter oxydans | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Gluconobacter oxydans | 4-nitrophenol + laurate | - |
? | |
7-aminocephalosporanic acid + H2O | - |
Gluconobacter oxydans | ? | - |
? | |
cephalosporin C + H2O | - |
Gluconobacter oxydans | ? | - |
? | |
phenyl acetate + H2O | - |
Gluconobacter oxydans | phenol + acetate | - |
? | |
phenyl acetate + H2O | - |
Gluconobacter oxydans 621H | phenol + acetate | - |
? | |
S-methyl thiobutanoate + H2O | - |
Gluconobacter oxydans | methanol + thiobutanoate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
native enzyme | Gluconobacter oxydans |
50 | - |
cross-linked enzyme aggregate | Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | after 5 min of incubation at 60°C, 20 min at 55°C and 40 min at 50°C, the immobilized cross-linked enzyme aggregate loses all of its initial activity. However, at 40°C, it is still slightly active after 7 h incubation | Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
1-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
9.3 | - |
S-methyl thiobutanoate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
9.8 | - |
1-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
15.2 | - |
2-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
17.6 | - |
4-nitrophenyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
20.81 | - |
cephalosporin C | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans | |
22.7 | - |
2-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
25.7 | - |
4-nitrophenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
42.7 | - |
cephalosporin C | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans | |
61.3 | - |
7-aminocephalosporanic acid | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans | |
69.5 | - |
7-aminocephalosporanic acid | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans | |
102 | - |
phenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
native enzyme | Gluconobacter oxydans |
8 | - |
cross-linked enzyme aggregate | Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
cephalosporin C | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans | |
0.9 | - |
7-aminocephalosporanic acid | immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C | Gluconobacter oxydans | |
1 | - |
S-methyl thiobutanoate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
20 | - |
1-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
110 | - |
1-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
220 | - |
2-naphthyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
240 | - |
4-nitrophenyl butyrate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
270 | - |
4-nitrophenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
1100 | - |
2-naphthyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
1480 | - |
phenyl acetate | immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C | Gluconobacter oxydans | |
2400 | - |
7-aminocephalosporanic acid | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans | |
2700 | - |
cephalosporin C | free enzyme, at pH 8.0 and 30°C | Gluconobacter oxydans |