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drug development
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resistance of strains to permethrin is associated with elevated levels of enzyme
analysis
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description of an easy and non-toxic method for large scale phenotyping and activity quantitation of arylesterase
analysis
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the enzyme has the potential to be used as a catalytic bioscavenger of nerve agents. Insect production of the enzyme may provide a source for both in vitro enzymatic and crystallographic studies and in vivo stability and anti-nerve agent efficacy testing
analysis
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quantification of arylesterase activity in routine clinical studies by monitoring the formation of acetic acid, upon the hydrolysis of phenyl acetate, using 10 microl of sample. The method accuracy is higher than 90% and intra-assay and inter-assay precisions are 96% and 95%, respectively. The procedure is suitable for use in human serum and heparinized plasma samples, while ethylenediaminetetra-acetic acid containing samples should be avoided
analysis
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use of 9-(4-chlorophenyloxycarbonyl)-10-methylacridinium triflate as a substrate for serum PON arylesterase activity assay. The apparent Km value of a serum sample for the substrate is 85 nmol/l, close to the Km value of recombinant human isoform PON1. Recombinant human PON1 in presence of CaCl2 shows at least 7.8 times selectivity over acetylcholinesterase and lipases. The method allows reliable, cost-saving, and specific determination in a buffer of physiological pH
medicine
aryl esterase activitiy correlates positively with total antioxidant status, high density lipoprotein and apolipoprotein A-I
medicine
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baseline and stimulated ARE activity is significantly lower in patients with chronic liver disease than in controls, serum ARE activity can be a suitable biomarker for the evaluation of the presence and severity of chronic liver damage
medicine
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both PON1 bioavailability and catalytic activity are decreased in children with autism spectrum disorders
medicine
diminished paraoxonase and arylesterase activity is associated with particular stage, grade and CA-125 level of ovarian cancer
medicine
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enzyme activity is reduced in subjects with type 1 and type 2 diabetes, the enzyme is associated with proteinuria in subjects with type 2 diabetes mellitus, there is an independent inverse association of enzyme mass with cystatin C Aboriginal subjects with type 2 diabetes
medicine
low paraoxonase-I activity in type 2 diabetes mellitus may contribute to increased cardiovascular risk via an effect on enhanced systemic low-grade inflammation
medicine
PON1 activity is lower in chronic hepatitis patients compared with the control subjects
medicine
PON1 moderately indicates cancer presence and regional metastasis, PON1 activity decreases in gastroesophageal cancers and corresponds to inflammation severity and cancer-related anemia, PON1's arylesterase activity reflects anemia severity, being correlated with hemoglobin, hematocrit, and iron
medicine
PON1 overexpression is associated with decreased diabetes-induced macrophage oxidative stress, decreased diabetes development, and decreased mortality
medicine
the arylesterase activity of PON1 is affected by critical ischemia of the lower limbs
medicine
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biologic monitoring for AREase status among pesticide handlers may be warranted to identify individuals who are at particularly high risk of organophosphate-related health effect
medicine
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serum ARE activity may be a suitable biomarker for identifying the presence and severity of chronic liver injury
medicine
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paraoxonase 1 significantly protects against sarin and soman exposure in guinea pigs and supports the development of paraoxonase 1 as a catalytic bioscavenger for protection against lethal exposure of chemical warfare nerve agents exposure
medicine
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paraoxonase 1 significantly protects against sarin and soman exposure in guinea pigs and supports the development of paraoxonase 1 as a catalytic bioscavenger for protection against lethal exposure of chemical warfare nerve agents exposure
medicine
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the lower enzyme activity in metabolic syndrome are considered an independent risk factor for cardiovascular disease
medicine
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ethanol consumption causes a significant decrease in liver paraoxonase activity. Gallic acid treatment partly restores this decreased paraoxonase activity. A gallic acid dose of 100 mg/kg shows highest restoring effect for paraoxonase activity. The activity of arylesterase is decreased in the ethanol group, but this decrease is not significant. Gallic acid treatment restores the loss of this activity due to ethanol exposure
medicine
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in patients with ankylosing spondylitis with active disease, levels of serum triglycerides, total cholesterol, low denstiy lipoprotein and malondialdehyde are significantly elevated while high density liporpoteon, paraoxonase PON1 and arylesterase ARE levels are lower than those with no active ankylosing spondylitis. Decrease in the PON1/ARE activity leading to generation of oxidative stress may play an important role in the pathogenesis of ankylosing spondylitis. Activity of PON1/ARE in patients with ankylosing spondylitis seems to be strictly correlated with the activity of the inflammatory process
medicine
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in patients with idiopathic Parkinson's disease, total antioxidant status levels are significantly lower than that of controls. Total oxidant status levels are higher than those of controls. Paraoxonase PON1 and arylesterase activities of patients are lower than those of controls. Serum levels of total and low density lipid cholesterol are significantly reduced in Parkinson's disease patients
medicine
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in patients with relapsing-remitting multiple sclerosis, total antioxidant status levels are significantly lower than that of controls. Total oxidant status levels of the patients are higher than that of controls. Paraoxonase PON1 and arylesterase activities of the patients are lower, but not significantly, than those of controls. There is no correlation between serum PON1 activity and oxidaive stress index in patients with relapsing-remitting multiple sclerosis. Endogenousantioxidants may have been exhausted by increased oxidative stress and additional antioxidant treatment might be beneficial for these patients
medicine
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in patients with selective serotonin reuptake inhibitor intoxication, the serum total antioxidant capacity levels and the paraoxonase and arylesterase activities are significantly lower, whereas the serum malondialdehyde levels are significantly higher than in the controls, indicating that decreased paraoxonase PON1 activity and increased oxidative stress represent alternative mechanisms in selective serotonin reuptake inhibitor toxicity
medicine
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incubation of serum or high density lipoprotein from healthy subjects with very low density lipoprotein significantly decreases serum paraoxonase 1 lactonase or arylesterase activities by up to 11% or 24%, and HDL-associated paraoxonase 1 lactonase or arylesterase activities by up to 32% or 46%, respectively. Very low density lipoprotein also inhibits recombinant paraoxonase 1 lactonase or arylesterase activities by up to 20% or 42%, respectively. Bezafibrate therapy to three hypertriglyceridemic patients (400 mg/day, for one month) significantly decreased serum triglyceride concentration by 67%, and increased serum high density lipoproteincholesterol levels by 48%. Paraoxonase 1 arylesterase or paraoxonase activities in the patients' high density lipoprotein fractions after drug therapy are significantly increased by 86-88%, as compared to paraoxonase 1 activities before treatment. High density lipoprotein-paraoxonase 1 protein levels significantly increased after bezafibrate therapy
medicine
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quantification of arylesterase activity in routine clinical studies by monitoring the formation of acetic acid, upon the hydrolysis of phenyl acetate, using 10 microl of sample. The method accuracy is higher than 90% and intra-assay and inter-assay precisions are 96% and 95%, respectively. The procedure is suitable for use in human serum and heparinized plasma samples, while ethylenediaminetetra-acetic acid containing samples should be avoided
medicine
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serum paraoxonase and arylesterase activities are significantly lower in patients with osteomyelitis compared to control individuals. Arylesterase activity is inversely correlated with triglyceride and cholesterol concentrations
medicine
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use of 9-(4-chlorophenyloxycarbonyl)-10-methylacridinium triflate as a substrate for serum PON arylesterase activity assay. The apparent Km value of a serum sample for the substrate is 85 nmol/l, close to the Km value of recombinant human isoform PON1. Recombinant human PON1 in presence of CaCl2 shows at least 7.8 times selectivity over acetylcholinesterase and lipases. The method allows reliable, cost-saving, and specific determination in a buffer of physiological pH
medicine
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paraoxonase-1 arylesterase activity is an independent predictor of myeloperoxidase levels in overweight patients with or without cardiovascular complications