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3.1.1.2: arylesterase

This is an abbreviated version!
For detailed information about arylesterase, go to the full flat file.

Word Map on EC 3.1.1.2

Reaction

a phenyl acetate
+
H2O
=
a phenol
 +
acetate

Synonyms

(R)-specific SGNH arylesterase, A-esterase, alpha-esterase, ARE, AREase, aromatic esterase, Aryl-ester hydrolase, aryldialkylphosphatase, arylesterase, DvvII, EST-1, EST-2, EST-3, ESt-A, Est0881, EstB, EstR5, G3C9 rePON1, gox0881, HDL-PON1, K-45, lp_1002, More, neuropathy target esterase-related esterase, NRE, NRECV, NTE-R1, NTE-related 1, NTE-related esterase, paraoxonase, paraoxonase 1, paraoxonase-1, paraoxonase/arylesterase, paraoxonase1, patatin-like phospholipase 7, phenyl acetate esterase, phenyl valerate esterase, PNPLA7, PON-aryl, PON1, PON1/Aryl, pyrethroid-resistance-associated esterase, Saci_2140, SacPox, serum paraoxonase/arylesterase 1, SGNH arylesterase, SGNH-arylesterase, SisLac, Sm23, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.2 arylesterase

Storage Stability

Storage Stability on EC 3.1.1.2 - arylesterase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 2 weeks
-
4°C, 50 mM Tris buffer, 50 mM NaCl, 1 mM CaCl2, 0.1% tergitol, pH 8.0, 1 month
4°C, 6 months, less than 15% loss of activity
4°C, purified enzyme is quite stable, showing no decrease in specific activity after storage for 1 month
4°C, purified PON1 in DEAE buffer, at least two weeks, remains stable
4°C, purified recombinant enzyme in 20 mM Tris pH 7.7, 1 mM CaCl2, at least one month, remains stable
4°C, purified recombinant PON1 preparations typically lose about 20% of the arylesterase activity within the first month after purification, compared with less than 10% loss for the enzyme purified from serum. There is no further significant loss of the PON1 activity afterward, suggesting the existence of two pools in the purified PON1, a labile one and a stable one
4°C, the enzyme activity is significantly lower after 48 h as well as after 1, 2 and 4 weeks storage when compared to enzyme activities assayed in fresh serum
-
4°C, the purified rPON1A maintains stability for several months when stored in the presence of 0.1% Tergitol NP-10 and 1 mM CaCl2
-