Literature summary for 3.1.1.2 extracted from

Hwang, H.; Kim, S.; Park, S.; Ngo, T.D.; Kim, K.K.; Kim, T.D.
Crystallization and diffraction analysis of Sm23: an SGNH-family arylesterase from Sinorhizobium meliloti 1021 (2011), Acta Crystallogr. Sect. F, 67, 572-574.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL1-Blue cells	Sinorhizobium meliloti

Crystallization (Commentary)

Crystallization (Comment)	Organism
microbatch method under oil, using 0.2 M magnesium formate as a precipitant	Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, His-tagged enzyme, SDS-PAGE	Sinorhizobium meliloti
24315	-	x * 24315, His-tagged enzyme, calculated from amino acid sequence	Sinorhizobium meliloti

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, column chromatography, gel filtration	Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme exhibits a substrate preference for short-chain fatty-acid esters such as C2 or C4 with an (R)-preference	Sinorhizobium meliloti	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 24000, His-tagged enzyme, SDS-PAGE	Sinorhizobium meliloti
?	x * 24315, His-tagged enzyme, calculated from amino acid sequence	Sinorhizobium meliloti

Synonyms

Synonyms	Comment	Organism
(R)-specific SGNH arylesterase	-	Sinorhizobium meliloti
Sm23	-	Sinorhizobium meliloti

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Release 2023.1 (January 2023)