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1.14.16.4: tryptophan 5-monooxygenase

This is an abbreviated version!
For detailed information about tryptophan 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.4

Reaction

L-tryptophan
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
5-hydroxy-L-tryptophan
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

hTPH2, indoleacetic acid-5-hydroxylase, L-tryptophan 5-hydroxylase, L-tryptophan hydroxylase, oxygenase, tryptophan 5-mono-, peripheral tryptophan hydroxylase, PTMO, putative tryptophan monooxygenase, TH2, TPH, TPH-1, TPH-2, TPH1, TPH2, TRpOH, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2, tryptophan hydroxylase I, tryptophan hydroxylase isoform 1, tryptophan hydroxylase isoform 2, tryptophan hydroxylase isoform2, tryptophan hydroxylase type I, tryptophan hydroxylase-1, tryptophan hydroxylase-2, tryptophan-5-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.4 tryptophan 5-monooxygenase

Cloned

Cloned on EC 1.14.16.4 - tryptophan 5-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a His-tagged version and a maltose binding protein-fusion protein are expressed in Escherichia coli
-
catalytic domain (DETLA1-100/DELTA415-445) of TPH1, expression in Escherichia coli
catalytic domain of TPH1 (DELTA1-99 TPH1 DELTA414-444) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells
catalytic domain of TPH2 (DELTA1-145 TPH2 DELTA460-490) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells
DNA and amio acid sequence determination and analysis of short and long isozymes TPH2, expression analysis in brain
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 Rosetta (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expression analysis of wild-type and mutant enzymes in brain samples
expression as maltose-binding protein fusion protein in Escherichia coli
expression as maltose-binding protein fusion protein in Escherichia coli BL21 (DE3)
expression in Escherichia coli
expression in escherichia coli and Pichia pastoris, His-Patch-thioredoxin-TPH, 6His-TPH or as a fusion protein with maltose-binding protein
-
expression in Escherichia coli BL21-CodonPlus(DE3)-RIL as His-tag fusion protein
-
expression in HeLa cells
expression in RN46A cells, transcriptional activity of the rat TPH2 promoter, overview
expression of full-length and N-terminal truncated enzyme in Escherichia coli
-
expression of GST-tagged TPH2 in Escherichia coli
-
expression of isozymes TPH1 and TPH2 in Escherichia coli
-
expression of TPH2 in COS cells
-
expression of wild-type and mutant isozymes TPH2 in Escherichia coli as His-tagged enzyme, and of wild-type TPH2 in HEK-293 cells
expression of wild-type and N- and C-terminal truncated mutant enzyme in Escherichia coli
-
expression of wild-type isozyme TPH1 in COS7 cells
-
expression of wild-type isozyme TPH2, and of TPH2 deletion mutants in COS7 cells
-
gene tph, DNA and amino acid sequence deterination and analysis, expression analysis, phylogenetic analysis
gene TPH, DNA and amino acid sequence determination and analysis
gene TPH1 genotyping of female and male Caucasian patients with major depression
gene TPH1, DNA and amino acid sequence determination and analysis, genotyping in Han Chinese subjects
gene TPH1, genotyping in Japanese population of healthy persons and shizophrenia patients
-
gene TPH2 is located on chromosome 12q15 comprising 11 exons, DNA and amino acid determination and analysis, genotyping of a population of alcohol-dependent patients of Caucasian origin from southern Germany
gene TPH2 located on the long arm of chromosome 12 at 12q21.1, DNA and amino acid sequence determination and analysis, genotyping
gene TPH2, cloning of four haplotypes of TPH2 into pcDNA3.1 and stable transfected into rat PC-12 cells, transient expressionin HEK-293 cells, quantitative real-time PCR expression analysis and comparison of mRNA stabilities, overview
gene TPH2, DNA and amino acid sequence determination and analysis of wild-type and mutant genes, genotyping, overview
gene TPH2, DNA and amino acid sequence determination and analysis, genotyping
gene TPH2, DNA and amino acid sequence determination and analysis, genotyping in Han Chinese subjects
gene TPH2, DNA and amino acid sequence determination and analysis, genotyping, overview
gene TPH2, DNA and amino acid sequence determination, analysis of the TPH2 promoter and bipartite NRSE motif, a transcription element in the TPH2 promoter, a bipartite variant interrupted by a 6-base insertion, that resembles the binding motif for RE-1 silencer of transcription, i.e. REST, also known as NRSF transcription factor, quantitative reverse transcription-PCR expression analysis, sequence comparisons
gene TPH2, DNA and amino acid sequence determination, identification of a transcription element in the TPH2 promoter, a bipartite variant interrupted by a 6-base insertion, that resembles the binding motif for RE-1 silencer of transcription, i.e. REST, also known as NRSF transcription factor, permutations of the motif reveal considerable flexibility in the juxtaposition of the two halves of bipartite NRSE, expressionin rat C6-glioma cells, quantitative reverse transcription-PCR expression analysis, sequence comparisons
gene tph2, expression of TPH2 wild-type and mutants in Escherichia coli and in HEK-293 cells
genes Tph1 and Tph2, expression analysis in wild-type and mutant mice, overview
-
genes TPH1 and TPH2, expression analysis, overview, expression of wild-type TPH1 and TPH2 in rat PC-12 cells, in vitro transcription and translation using reticulocyte lysate, the TPH2 extended N-terminal domain plays a role in modulating the enzyme expression, expression of mutant HA-TPH2-(1-41)-TPH1 in HEK-293 amd PC-12 cells, the expression level of the chimeric protein is significantly decreased compared with TPH1 in both cell lines and is more similar to that of TPH2, the N-terminal regulatory determinant is transferable, overview
genotyping of borderline personality disorder patients and healthy persons
-
glutathione S-transferase-fusion protein expressed in Escherichia coli, expressed in HEK293-cell, eGFP-fusion protein expressed in HEK293-cell
-
high yield expression of the catalytic domain of chicken TPH isoform 1, comprising residues 1-100 and 415-445, fused to ubiquitin in Escherichia coli strain BL21(DE3)
His-tagged enzyme, expression in Escherichia coli
into pT vector and linearized with Nco I prior to antisense transcription with SP6
-
isozyme TPH1, DNA and amino acid sequence determination and analysis, tissue-specific and developmental expression analysis, expression of His6-tagged TPH1 in COS7 cells
isozyme TPH1, DNA and amino acid sequence determination and analysis, TPH1 is located on chromosome 11, expression analysis
isozyme TPH2, DNA and amino acid sequence determination and analysis, tissue-specific and developmental expression analysis, expression of His6-tagged TPH2 in COS7 cells
isozyme TPH2, DNA and amino acid sequence determination and analysis, TPH2 is on chromosome 12, expression analysis
pBR322 containing the open reading frame of PTMO, PCR products inserted into the EcoRV site of pBluescript vector, expressed from vector pET 22b(+) in Escherichia coli BL21(DE3)
-
PCR products ligated and cloned into pGEM-T easy vector
secondary structure of TPH1 RNA, overview
-
TPH1 and TPH2 genotyping
TPH1, expression in Escherichia coli
-
TPH1, genotyping in Korean population with major depressive disorder patients, overview
TPH2, DNA and amino acid sequence determination and analysis, genotyping
TPH2, DNA and amino acid sequence determination and analysis, genotyping, expression of mutant Q468R in HeLa cells, overview
-
TPH2, expression in Escherichia coli
-
transcriptional activity of the rat TPH2 promoter in GH4C1 cells, RN46A cells, and L6 myoblasts, overview
-
truncated version expressed in Escherichia coli
-
wild-type and missense mutants from vector pETM41 for 6 x His-MBP-TPH2 expression in Escherichia coli BL21-Codon Plus (DE3)-RIPL cells, and vector pcDNA5/FRT, for expression in a cell free expression system and in HEK-293 cells. Rabbit reticulocyte lysate used to express TPH2 wild-type and missense variants