Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.16.4: tryptophan 5-monooxygenase

This is an abbreviated version!
For detailed information about tryptophan 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.4

Reaction

L-tryptophan
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
5-hydroxy-L-tryptophan
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

hTPH2, indoleacetic acid-5-hydroxylase, L-tryptophan 5-hydroxylase, L-tryptophan hydroxylase, oxygenase, tryptophan 5-mono-, peripheral tryptophan hydroxylase, PTMO, putative tryptophan monooxygenase, TH2, TPH, TPH-1, TPH-2, TPH1, TPH2, TRpOH, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2, tryptophan hydroxylase I, tryptophan hydroxylase isoform 1, tryptophan hydroxylase isoform 2, tryptophan hydroxylase isoform2, tryptophan hydroxylase type I, tryptophan hydroxylase-1, tryptophan hydroxylase-2, tryptophan-5-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.4 tryptophan 5-monooxygenase

Purification

Purification on EC 1.14.16.4 - tryptophan 5-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine-agarose
-
acid treatment, ammonium sulfate, Sepharose CL-6B, DEAE-sepharose, butyl-Sepharose, Toyopearl
-
brain enzyme, calcium phosphate gel, dimethyltetrahydropteridine-agarose
by a rapid batch method
by gel filtration and ultrafiltration
catalytic domain (DETLA1-100/DELTA415-445) of TPH1
dextrin Sepharose column chromatography and Superdex 200 gel filtration
-
enzyme from pineal gland, ammonium sulfate, hydroxylapatite, P-cellulose, DEAE-cellulose, Sephadex G-200
-
glutathione-Sepharose column chromatography, and gel filtration
-
hindbrain enzyme, calcium phosphate gel, polyethylene glycol 6000, Sepharose 6B, DEAE-cellulose
-
HisTrap column chromatography and Superdex 200 gel filtration
-
Ni-affinity chromatography
-
Ni-NTA column chromatography and Superdex SPX200 gel filtration
-
one chromatography step, 98% homogeneity
-
purified and cleaved from its fusion tag
-
purified by amylose resin affinity chromatography
recombinant catalytic domain of chicken TPH isoform 1 from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration to homogeneity, release from the fusion by in vivo cleavage by a co-expressed ubiquitin-specific, carboxy-terminal protease
recombinant enzyme
recombinant full-length and N-terminal truncated enzyme
-
recombinant GST-tagged TPH2 from Escherichia coli by glutathione affinity chromatography
-
recombinant His-tagged enzyme, Nickel affinity chromatography
recombinant His-tagged wild-type and mutant TPH2s from Escherichia coli
recombinant His6-MBP-fusion TPH2 wild-type and mutants from Escherichia coli by affinity chromatography, fusion protein cleavage, and gel filtration
recombinant isozymes TPH1 and TPH2 from Escherichia coli by pterin affinity chromatography
-
recombinant N- and C-terminal truncated mutant enzyme
-
recombinant pineal enzyme, ammonium sulfate, Pterin-agarose, Sepharose
-
tryptophan hydroxylase form I and II, pH 4.8, ammonium sulfate, gel filtration, DEAE-Sepharose, 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine-agarose
-
two chromatography steps