Literature summary for 1.14.16.4 extracted from

Hasegawa, H.; Ichiyama, A.
Distinctive iron requirement of tryptophan 5-monooxygenase: TPH1 requires dissociable ferrous iron (2005), Biochem. Biophys. Res. Commun., 338, 277-284.

Search for more literature for 1.14.16.4

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	30 mM, 79.3% activity compared to activation with dithiothreitol	Mus musculus
2-mercaptoethylamine	30 mM, 93.8% activity compared to activation with dithiothreitol	Mus musculus
2-mercaptopropionate	30 mM, 11.7% activity compared to activation with dithiothreitol	Mus musculus
3-Mercaptopropionate	30 mM, 22% activity compared to activation with dithiothreitol	Mus musculus
dithiothreitol	10 mM	Mus musculus
glutathione	30 mM, 16.1% activity compared to activation with dithiothreitol	Mus musculus
L-cysteine	30 mM, 79.5% activity compared to activation with dithiothreitol	Mus musculus
N-acetylcysteine	30 mM, 31.2% activity compared to activation with dithiothreitol	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21-CodonPlus(DE3)-RIL as His-tag fusion protein	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
delta1-150	deletion of the N-terminal regulatory domain. Increased solubility when expressed in Escherichia coli.	Homo sapiens
delta1-150/421-444	deletion of the N-terminal regulatory domain and the C-terminal tetramerisation domain. Increased solubility when expressed in Escherichia coli. The mutant protein shows a 2fold increase of the Km for L-tryptophan and a 3fold decrease of the Km for (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin	Homo sapiens

General Stability

General Stability	Organism
The half-life of the protein is 30 min at 37°C, while the N-terminal and the N- and C-terminal truncated mutants exhibit half-lives of 203 min and 147 min, respectively	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01789	-	(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin	-	Homo sapiens
0.02426	-	L-tryptophan	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required for activity	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
59000	-	SDS-PAGE	Homo sapiens
236000	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity chromatography	Homo sapiens
one chromatography step, 98% homogeneity	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5	-	-	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2	-	Mus musculus	5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2	-	Homo sapiens	5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 59000, gel filtration and SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
TPH1	-	Mus musculus
TPH2	-	Homo sapiens
tryptophan 5-monooxygenase	-	Mus musculus
tryptophan hydroxylase	-	Mus musculus
tryptophan hydroxylase 2	-	Homo sapiens

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Release 2023.1 (January 2023)