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(2-chloroacetamido)(phenyl)acetate + H2O
chloroacetate + amino(phenyl)acetate
-
-
-
-
?
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
2-acetylamino-3-((1S,2S)-2-hydroxycyclooctylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclooctyl]cysteine + acetate
-
-
-
?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
3-(2-furyl)acryloyl-L-methionine + H2O
3-(2-furyl)acrylate + L-methionine
-
-
-
?
8-methyl-N-vanillyl-6-nonenamide + H2O
?
-
i.e. capsaicin
-
-
?
acetyl-DL-alloisoleucine + H2O
acetate + DL-alloisoleucine
-
-
-
-
?
acetyl-DL-ethionine + H2O
acetate + DL-ethionine
-
-
-
-
?
acetyl-DL-isoleucine + H2O
acetate + DL-isoleucine
-
-
-
-
?
acetyl-L-beta amino acid + H2O
acetate + L-beta amino acid
-
-
-
-
?
acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
alpha,beta-dichloroacetyl-DL-diaminopropionic acid + H2O
?
-
-
-
-
?
benzoyl-DL-alanine + H2O
benzoate + DL-alanine
-
-
-
-
?
benzoyl-DL-leucinamide + H2O
benzoate + DL-leucinamide
-
-
-
-
?
benzoyl-DL-methionine + H2O
benzoate + DL-methionine
-
-
-
-
?
benzoyl-DL-phenylalanine + H2O
benzoate + DL-phenylalanine
benzoyl-DL-valine + H2O
benzoate + DL-valine
-
-
-
-
?
benzoyl-L-leucine + H2O
benzoate + L-leucine
-
-
-
-
?
benzyloxycarbonglycyl-L-leucine + H2O
?
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + ?
-
-
-
-
?
chloracetyl-DL-aminoheptylic acid + H2O
chloroacetate + DL-aminoheptanoate
-
-
-
-
?
chloroacetyl-DL-allothreonine + H2O
chloroacetate + DL-allothreonine
-
-
-
-
?
chloroacetyl-DL-serine + H2O
chloroacetate + DL-serine
-
-
-
-
?
chloroacetyl-DL-threonine + H2O
chloroacetate + DL-threonine
-
-
-
-
?
chloroacetyl-L-4-fluorophenylalanine + H2O
chloroacetate + L-4-fluorophenylalanine
-
-
-
-
?
chloroacetyl-L-4-methoxyphenylalanine + H2O
chloroacetate + L-4-methoxyphenylalanine
-
-
-
-
?
chloroacetyl-L-beta amino acid + H2O
chloroacetate + L-beta amino acid
-
-
-
-
?
chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
-
-
-
-
?
dichloroacety-DL-phenylalanine + H2O
dichloroacetate + DL-phenylalanine
-
-
-
-
?
dichloroacetyl-DL-alanine + H2O
dichloroacetate + DL-alanine
-
-
-
-
?
dichloroacetyl-DL-ornithine + H2O
dichloroacetate + DL-ornithine
-
-
-
-
?
DL-N-acetyl-D,L-3-(4-thiazolyl)alanine + H2O
L-3-(4-thiazolyl)alanine + D-N-acetyl-D-3-(4-thiazolyl)alanine
-
-
-
-
?
fluoroacetyl-DL-alanine + H2O
fluoroacetate + DL-alanine
-
-
-
-
?
fluoroacetyl-L-phenylalanine + H2O
fluoroacetate + L-phenylalanine
-
-
-
-
?
formyl-DL-alanine + H2O
formate + DL-alanine
-
-
-
-
?
formyl-DL-phenylalanine + H2O
formate + L-phenylalanine
-
-
-
-
?
furylacryloyl-L-methionine + H2O
?
-
-
-
-
?
glycyl-L-methionine + H2O
glycine + L-methionine
-
-
-
-
?
glycyl-L-tyrosine + H2O
glycine + L-tyrosine
-
-
-
-
?
hydroxyacetyl-DL-alanine + H2O
hydroxyacetate + DL-alanine
-
isolated as the barium salt
-
-
?
hydroxyacetyl-DL-phenylalanine + H2O
hydroxyacetate + DL-phenylalanine
-
-
-
-
?
iodoacetyl-DL-alanine + H2O
iodoacetate + DL-alanine
-
-
-
-
?
iodoacetyl-DL-phenylalanine + H2O
iodoacetate + DL-phenylalanine
-
-
-
-
?
L-methionylglycine + H2O
glycine + L-methionine
-
-
-
-
?
m-nitrobenzoyl-DL-alanine + H2O
3-nitrobenzoate + DL-alanine
-
-
-
-
?
methyl-mercaptoacetyl-DL-alanine + H2O
methylmercaptoacetate + DL-alanine
-
-
-
-
?
methylmercaptoacetyl-DL-phenylalanine + H2O
methylmercaptoacetate + DL-phenylalanine
-
-
-
-
?
N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
(E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
N-2,2-dimethylpropionyl-L-methionine + H2O
2,2-dimethyl-propionate + L-methionine
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
3-methyl-3-hydroxy-hexanoate + L-glutamine
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
N-acetyl-D-aspartate + H2O
?
-
-
-
-
?
N-acetyl-D-Homophenylalanine + H2O
acetate + D-Homophenylalanine
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
N-acetyl-DL-methionine + H2O
N-acetyl-D-methionine + L-methionine + acetic acid
-
-
-
-
?
N-acetyl-DL-norleucine + H2O
DL-norleucine + acetate
N-acetyl-DL-phenylglycine + H2O
DL-phenylglycine + acetate
-
only D-isomer hydrolyzed
-
-
?
N-acetyl-DL-proline + H2O
acetate + DL-proline
-
-
-
-
?
N-acetyl-DL-serine + H2O
acetate + DL-serine
-
-
-
-
?
N-acetyl-DL-Trp + H2O
acetate + DL-Trp
-
-
-
-
?
N-acetyl-DL-tyrosine + H2O
acetate + DL-tyrosine
N-Acetyl-Gly + H2O
acetate + Gly
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
N-acetyl-L-2-aminopentanoate + H2O
acetate + L-2-aminopentanoate
-
-
-
-
?
N-acetyl-L-Ala + H2O
acetate + L-Ala
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
N-acetyl-L-alanine + H2O
L-alanine + acetate
N-acetyl-L-amino acid + H2O
carboxylic acid + L-amino acid
N-acetyl-L-Arg + H2O
acetate + L-Arg
-
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
N-acetyl-L-Asn + H2O
acetate + L-Asn
-
-
-
?
N-acetyl-L-Asp + H2O
acetate + L-Asp
-
-
-
?
N-acetyl-L-asparagine + H2O
acetate + L-asparagine
N-acetyl-L-aspartate + H2O
acetate + L-aspartate
-
no activity of wild type protein, mutant E167R protein shows activity
-
-
?
N-acetyl-L-Cys + H2O
acetate + L-Cys
N-acetyl-L-Cys + H2O
L-Cys + acetate
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
N-acetyl-L-ethionine + H2O
acetate + L-ethionine
N-acetyl-L-Gln + H2O
acetate + L-Gln
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
N-acetyl-L-histidine + H2O
acetate + L-histidine
N-acetyl-L-Homophenylalanine + H2O
acetate + L-Homophenylalanine
-
-
-
-
?
N-acetyl-L-isoleucine + H2O
acetate + L-isoleucine
-
-
-
?
N-acetyl-L-Leu + H2O
acetate + L-Leu
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
N-acetyl-L-Met + H2O
acetate + L-Met
N-acetyl-L-Met + H2O
L-Met + acetate
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
N-acetyl-L-methionine + H2O
acetate + L-methionine
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
N-acetyl-L-norleucine + H2O
acetate + L-norleucine
N-acetyl-L-ornithine + H2O
acetate + L-ornithine
-
-
-
-
?
N-acetyl-L-Phe + H2O
acetate + L-Phe
-
-
-
?
N-acetyl-L-Phe methyl ester + H2O
acetate + L-Phe methyl ester
-
the enzyme also shows esterase activity with N-acetyl-L-Phe methyl ester
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
N-acetyl-L-phenylalanine methyl ester + H2O
acetate + L-phenylalanine methyl ester
-
-
-
-
?
N-acetyl-L-serine + H2O
acetate + L-serine
-
immobilised enzyme
-
-
?
N-acetyl-L-threonine + H2O
acetate + L-threonine
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
N-acetyl-L-tyrosine + H2O
tyrosine + acetate
-
-
-
-
?
N-acetyl-L-Val + H2O
acetate + L-Val
-
-
-
?
N-acetyl-L-Val + H2O
L-Val + acetate
-
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
N-acetyl-L-valine + H2O
L-valine + acetate
N-acetyl-O-benzyl-DL-serine + H2O
acetate + O-benzyl-DL-serine
-
no hydrolysis with immobilized enzyme
-
-
?
N-acetyl-S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + H2O
acetate + S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
-
-
?
N-acetyl-S-(1,2,2-trichlorovinyl)-L-cysteine + H2O
acetate + S-(1,2,2-trichlorovinyl)-L-cysteine
-
-
-
?
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine + H2O
acetate + S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine
-
-
-
?
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine + H2O
acetate + S-(1,2-dichlorovinyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine + H2O
acetate + S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2,2-dichlorovinyl)-L-cysteine + H2O
acetate + S-(2,2-dichlorovinyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine + H2O
acetate + S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine + H2O
acetate + S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2-chlorobenzyl)-L-cysteine + H2O
acetate + S-(2-chlorobenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-(2-fluorobenzyl)-L-cysteine + H2O
acetate + S-(2-fluorobenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-(3-fluorobenzyl)-L-cysteine + H2O
acetate + S-(3-fluorobenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-(4-bromobenzyl)-L-cysteine + H2O
acetate + S-(4-bromobenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-(4-chlorobenzyl)-L-cysteine + H2O
acetate + S-(4-chlorobenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-(4-methoxybenzyl)-L-cysteine + H2O
acetate + S-(4-methoxybenzyl)-L-cysteine
-
-
-
?
N-acetyl-S-1,2-dichlorovinyl-L-cysteine + H2O
acetate + S-1,2-dichlorovinyl-L-cysteine
-
-
-
-
?
N-acetyl-S-benzyl-L-cysteine + H2O
acetate + S-benzyl-L-cysteine
N-acetyl-S-DL-benzylcysteine + H2O
acetate + S-DL-benzylcysteine
-
-
-
-
?
N-acetylglycine + H2O
acetate + glycine
N-acetyllysine + H2O
lysine + acetate
-
-
-
-
?
N-acetylmethionine + H2O
acetate + L-methionine
-
preferred substrate
-
-
?
N-acetylpipecolic acid + H2O
acetate + L-piperidine-2-carboxylate
-
-
-
-
?
N-acetylseleno-L-methionine + H2O
acetate + L-selenomethionine
-
-
-
-
?
N-acyl-L-amino acid + H2O
carboxylate + L-amino acid
-
-
-
?
N-alpha-acetyl-L-alanine + H2O
acetate + L-alanine
N-alpha-acetyl-L-aspartate + H2O
acetate + L-aspartate
N-alpha-acetyl-L-cysteine + H2O
acetate + L-cysteine
N-alpha-acetyl-L-glutamate + H2O
acetate + L-glutamate
N-alpha-acetyl-L-isoleucine + H2O
acetate + L-isoleucine
-
-
-
-
?
N-alpha-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-alpha-acetyl-L-lysine + H2O
acetate + L-lysine
N-alpha-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
N-alpha-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
-
?
N-alpha-acetyl-L-proline + H2O
acetate + L-proline
-
-
-
-
?
N-alpha-acetyl-L-serine + H2O
acetate + L-serine
-
-
-
-
?
N-alpha-acetyl-L-threonine + H2O
acetate + L-threonine
-
-
-
-
?
N-alpha-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
-
-
-
-
?
N-alpha-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
-
?
N-alpha-acetyl-L-valine + H2O
acetate + L-valine
-
-
-
-
?
N-alpha-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
-
-
-
-
?
N-alpha-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
-
-
-
-
?
N-benzoyl-L-arginine + H2O
benzoate + L-arginine
-
immobilised enzyme
-
-
?
N-benzoyl-L-glutamate + H2O
benzoate + L-glutamate
N-benzoyl-L-isoleucine + H2O
benzoate + L-isoleucine
-
-
-
-
?
N-benzoyl-L-leucine + H2O
benzoate + L-leucine
-
immobilised enzyme
-
-
?
N-benzoyl-L-methionine + H2O
benzoate + L-methionine
N-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
N-benzoyl-L-tert-leucine + H2O
benzoate + L-tert-isoleucine
-
-
-
-
?
N-benzoyl-L-threonine + H2O
benzoate + L-threonine
-
immobilised enzyme
-
-
?
N-benzoyl-L-valine + H2O
benzoate + L-valine
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Gly + H2O
benzyl hydrogencarbonate + L-Phe-Gly
-
-
-
-
?
N-benzyloxycarbonyl-L-phenylalanine + H2O
benzyl hydrogencarbonate + L-phenylalanine
-
-
-
-
?
N-benzyloxycarbonyl-L-phenylalanine + H2O
benzyloxycarbonate + L-phenylalanine
-
33.9% of the activity with N-acetyl-L-phenylalanine. no activity with N-benzyloxycarbonyl-D-phenylalanine
-
-
?
N-benzyloxycarbonyl-L-valine + H2O
benzyl hydrogencarbonate + L-valine
-
-
-
-
?
N-bromoacetyl-DL-alanine + H2O
bromoacetate + DL-alanine
-
-
-
-
?
N-bromoacetyl-DL-phenylalanine + H2O
bromoacetate + DL-phenylalanine
-
-
-
-
?
N-butanoyl-L-methionine + H2O
butanoate + L-methionine
N-butyryl-D-aspartate + H2O
butanoate + D-aspartate
-
-
-
-
?
N-butyryl-L-Met + H2O
butanoate + L-Met
-
-
-
?
N-butyryl-L-methionine + H2O
butyrate + L-methionine
-
-
-
?
N-carbobenzyloxy-L-glutamine + H2O
?
N-CBZ-Gly-Ala + H2O
?
-
-
-
-
?
N-Cbz-Gly-L-Ala + H2O
?
-
-
-
-
?
N-CBZ-L-phenylalanine + H2O
? + L-phenylalanine
-
immobilised enzyme
-
-
?
N-chloroacetyl-2-amino-2-cyclohexylacetate + H2O
chloroacetate + 2-amino-2-cyclohexylacetate
-
-
-
-
?
N-chloroacetyl-2-amino-3-cyclohexylpropionate + H2O
chloroacetate + 2-amino-3-cyclohexylpropionate
-
-
-
-
?
N-chloroacetyl-2-amino-4-cyclohexylbutyrate + H2O
chloroacetate + 2-amino-4-cyclohexylbutyrate
-
-
-
-
?
N-chloroacetyl-2-aminobutyrate + H2O
chloroacetate + 2-aminobutyrate
-
-
-
-
?
N-chloroacetyl-2-aminocaprylate + H2O
chloroacetate + 2-aminocaprylate
-
-
-
-
?
N-chloroacetyl-DL-alanine + H2O
chloroacetate + alanine
N-chloroacetyl-DL-aspartate + H2O
chloroacetate + DL-aspartate
-
-
-
-
?
N-chloroacetyl-DL-glutamine + H2O
chloroacetate + DL-glutamine
-
-
-
-
?
N-chloroacetyl-DL-norvaline + H2O
chloroacetate + DL-norvaline
-
N-acylated racemic amino acids, pure acylated D forms hydrolyzed 10000-40000 times more slowly than corresponding pure acylated L isomers
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
N-chloroacetyl-DL-proline + H2O
chloroacetate + DL-proline
-
-
-
-
?
N-chloroacetyl-DL-tyrosine + H2O
chloroacetate + DL-tyrosine
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
N-chloroacetyl-L-methionine + H2O
chloroacetate + L-methionine
N-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
N-chloroacetylglutamine-p-aminobenzoate + H2O
?
N-chloroacetylglycine + H2O
chloroacetate + glycine
N-chloroacetyltryptophan + H2O
chloroacetate + tryptophan
N-cyclohexanoyl-L-methionine + H2O
cyclohexanecarboxylate + L-methionine
-
-
-
-
?
N-cyclohexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
?
N-cyclopentanoyl-L-methionine + H2O
cyclopentanecarboxylate + L-methionine
-
-
-
-
?
N-cyclopentanoyl-L-methionine + H2O
pentanoate + L-methionine
-
-
-
?
N-decanoyl-L-glutamine + H2O
decanoate + L-glutamine
N-decanoyl-L-Met + H2O
decanoate + L-Met
-
-
-
?
N-decanoyl-L-methionine + H2O
decanoate + L-methionine
-
-
-
?
N-dichloroacetyl-DL-lysine + H2O
dichloroacetate + DL-lysine
-
-
-
-
?
N-dichloroacetylnorleucine + H2O
dichloroacetate + norleucine
-
-
-
-
?
N-formyl-D-aspartate + H2O
formate + D-aspartate
-
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
N-formyl-Lmethionine + H2O
formate + L-methionine
-
-
-
-
?
N-formylmethionine + H2O
formate + L-methionine
-
substrate synthesis and purification, overview
-
-
?
N-glycyl-D-aspartate + H2O
glycine + D-aspartate
-
-
-
-
?
N-hexanoyl-L-Met + H2O
hexanoate + L-Met
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
N-isobutanoyl-L-methionine + H2O
isobutanoate + L-methionine
N-isopentanoyl-L-methionine + H2O
isopentanoate + L-methionine
N-lauroyl-Gly + H2O
laurate + Gly
-
-
-
?
N-lauroyl-glycine + H2O
laurate + glycine
-
-
-
-
?
N-lauroyl-L-Ala + H2O
laurate + L-Ala
-
-
-
?
N-lauroyl-L-alanine + H2O
laurate + L-alanine
-
-
-
-
?
N-lauroyl-L-Arg + H2O
laurate + L-Arg
-
-
-
?
N-lauroyl-L-arginine + H2O
laurate + L-arginine
-
-
-
-
?
N-lauroyl-L-Asn + H2O
laurate + L-Asn
-
-
-
?
N-lauroyl-L-asparagine + H2O
laurate + L-asparagine
-
-
-
-
?
N-lauroyl-L-aspartate + H2O
laurate + L-aspartate
-
-
-
-
?
N-lauroyl-L-Cys + H2O
laurate + L-Cys
-
-
-
?
N-lauroyl-L-glutamate + H2O
laurate + L-glutamate
-
-
-
-
?
N-lauroyl-L-glutamine + H2O
laurate + L-glutamine
N-lauroyl-L-glutaminyl-glycine + H2O
laurate + L-glutaminyl-glycine
-
-
-
-
?
N-lauroyl-L-glutaminyl-L-lysine + H2O
laurate + L-glutaminyl-L-lysine
-
-
-
-
?
N-lauroyl-L-glutamyl-glycine + H2O
laurate + L-glutamyl-glycine
-
-
-
-
?
N-lauroyl-L-His + H2O
laurate + L-His
-
-
-
?
N-lauroyl-L-histidine + H2O
laurate + L-histidine
-
-
-
-
?
N-lauroyl-L-isoleucine + H2O
laurate + L-isoleucine
-
-
-
-
?
N-lauroyl-L-leucine + H2O
laurate + L-leucine
-
-
-
-
?
N-lauroyl-L-Lys + H2O
laurate + L-Lys
-
-
-
?
N-lauroyl-L-Met + H2O
laurate + L-Met
-
-
-
?
N-lauroyl-L-methionine + H2O
laurate + L-methionine
-
-
-
-
?
N-lauroyl-L-phenylalanine + H2O
laurate + L-phenylalanine
-
-
-
-
?
N-lauroyl-L-Ser + H2O
laurate + L-Ser
-
-
-
?
N-lauroyl-L-serine + H2O
laurate + L-serine
-
best substrate
-
-
?
N-lauroyl-L-threonine + H2O
laurate + L-threonine
-
-
-
-
?
N-lauroyl-L-tryptophan + H2O
laurate + L-tryptophan
-
-
-
-
?
N-lauroyl-L-tyrosine + H2O
laurate + L-tyrosine
-
-
-
-
?
N-lauroyl-L-valine + H2O
laurate + L-valine
-
-
-
-
?
N-lauryl-glycine + H2O
laurate + glycine
-
-
-
?
N-lauryl-L-alanine + H2O
laurate + L-alanine
-
-
-
?
N-lauryl-L-cysteine + H2O
laurate + L-cysteine
-
-
-
?
N-lauryl-L-lysine + H2O
laurate + L-lysine
-
-
-
?
N-lauryl-L-methionine + H2O
laurate + L-methionine
-
-
-
?
N-lauryl-L-serine + H2O
laurate + L-serine
-
-
-
?
N-linolenoyl-L-glutamine + H2O
linolenic acid + L-glutamine
-
-
-
?
N-myristoyl-L-Met + H2O
myristate + L-Met
-
-
-
?
N-myristoyl-L-methionine + H2O
myristate + L-methionine
-
-
-
?
N-octanoyl-L-Met + H2O
octanoate + L-Met
-
-
-
?
N-octanoyl-L-methionine + H2O
octanoate + L-methionine
-
-
-
?
N-palmitoyl-L-Met + H2O
palmitate + L-Met
-
-
-
?
N-palmitoyl-L-methionine + H2O
palmitate + L-methionine
-
-
-
?
N-pentanoyl-L-methionine + H2O
pentanoate + L-methionine
N-propionyl-D-aspartate + H2O
proipionate + D-aspartate
-
-
-
-
?
N-propionyl-L-methionine + H2O
propionate + L-methionine
N-t-BOC-L-phenylalanine + H2O
? + L-phenylalanine
-
immobilised enzyme
-
-
?
N-tert-butyloxycarbonyl-L-phenylalanine + H2O
tert-butyloxycarboxylate + L-phenylalanine
-
-
-
-
?
N-trifluoroacetyl 2-trifluoromethylalanine + H2O
trifluoroacetate + 2-trifluoromethylalanine
-
-
-
-
?
Nalpha-acetylmethionine + H2O
acetate + L-methionine
-
-
-
-
?
Nalpha-lauroyl-L-lysine + H2O
laurate + L-lysine
-
-
-
-
?
Nepsilon-lauroyl-L-lysine + H2O
laurate + L-lysine
-
-
-
-
?
O-methyl-N-acetyl-DL-serine + H2O
acetate + O-methyl-DL-serine
-
-
-
-
?
p-bromobenzoyl-DL-alanine + H2O
4-bromobenzoate + DL-alanine
-
-
-
-
?
p-chlorobenzoyl-DL-alanine + H2O
4-chlorbenzoate + DL-alanine
-
-
-
-
?
p-fluorobenzoyl-DL-alanine + H2O
4-fluorobenzoate + DL-alanine
-
-
-
-
?
p-iodobenzoyl-DL-alanine + H2O
4-iodobenzoate + DL-alanine
-
-
-
-
?
p-nitrobenzoyl-DL-alanine + H2O
4-nitrobenzoate + DL-alanine
-
-
-
-
?
p-tolyl-DL-alanine + H2O
?
-
-
-
-
?
palmitoyl-L-aspartate + H2O
palmitate + L-aspartate
-
-
-
-
?
palmitoyl-L-phenylalanine + H2O
palmitate + L-phenylalanine
-
-
-
-
?
propionyl-DL-alanine + H2O
propanoate + DL-alanine
-
-
-
-
?
S-(1,1,2,2-tetrafluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(-1,1,2,2-terafluoroethyl)-L-cysteine
-
-
-
-
?
S-(2-bromo-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(2-bromo-1,1,2-trifluoroethyl)-L-cysteine
-
-
-
-
?
S-(2-chloro-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
-
-
-
?
S-ethyl-N-acetyl-L-cysteine + H2O
acetate + S-ethyl-L-cysteine
-
-
-
-
?
S-isobutyl-N-acetyl-L-cysteine + H2O
?
-
-
-
-
?
S-isopropyl-N-acetyl-L-cysteine + H2O
acetate + S-isopropyl-L-cysteine
-
-
-
-
?
S-methyl-N-acetyl-L-cysteine + H2O
acetate + S-methyl-L-cysteine
-
-
-
-
?
S-n-butyl-N-acetyl-L-cysteine + H2O
acetate + S-n-butyl-L-cysteine
-
-
-
-
?
S-n-propyl-N-acetyl-L-cysteine + H2O
acetate + S-n-propyl-L-cysteine
-
-
-
-
?
trifluoroacetyl-DL-alanine + H2O
trifluoroacetate + DL-alanine
-
trifluoroacetyl derivatives are most rapidly hydrolyzed
-
-
?
trifluoroacetyl-DL-phenylalanine + H2O
trifluoroacetate + DL-phenylalanine
-
-
-
-
?
additional information
?
-
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
-
-
-
?
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
-
-
?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
-
-
-
?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
-
-
?
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
-
-
-
?
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
-
-
?
benzoyl-DL-phenylalanine + H2O
benzoate + DL-phenylalanine
-
-
-
-
?
benzoyl-DL-phenylalanine + H2O
benzoate + DL-phenylalanine
-
-
-
-
?
chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
r
N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
(E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
-
-
-
-
?
N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
(E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
-
-
-
-
?
N-2,2-dimethylpropionyl-L-methionine + H2O
2,2-dimethyl-propionate + L-methionine
-
-
-
?
N-2,2-dimethylpropionyl-L-methionine + H2O
2,2-dimethyl-propionate + L-methionine
-
-
-
-
?
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
3-methyl-3-hydroxy-hexanoate + L-glutamine
-
-
-
-
?
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
3-methyl-3-hydroxy-hexanoate + L-glutamine
-
-
-
-
?
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
-
a metabolite of a xenobiotic trichloroethylene, substrate of AA3
-
-
?
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
-
a metabolite of a xenobiotic trichloroethylene, substrate of AA3
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
D-isomer, N-acetyl-D-aspartate as inducer
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
-
pure acylated D-forms hydrolyzed 10000-40000 times more slowly than corresponding pure acylated L-isomers
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
288852, 288853, 288856, 288857, 288859, 288860, 288862, 288863, 288864, 288866, 288867, 288868, 288869, 288870, 288871, 288872, 288873, 288874 -
-
?
N-acetyl-DL-norleucine + H2O
DL-norleucine + acetate
-
-
-
-
?
N-acetyl-DL-norleucine + H2O
DL-norleucine + acetate
-
-
-
-
?
N-acetyl-DL-tyrosine + H2O
acetate + DL-tyrosine
-
-
-
-
?
N-acetyl-DL-tyrosine + H2O
acetate + DL-tyrosine
-
prefers D-isomer
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-alanine + H2O
L-alanine + acetate
-
-
-
-
?
N-acetyl-L-amino acid + H2O
carboxylic acid + L-amino acid
-
-
-
-
?
N-acetyl-L-amino acid + H2O
carboxylic acid + L-amino acid
-
highly enantioselective hydrolysis of esters and amides, the L-configuration is preferred, overview
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
-
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
-
-
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
-
-
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
-
-
-
?
N-acetyl-L-asparagine + H2O
acetate + L-asparagine
-
-
-
?
N-acetyl-L-asparagine + H2O
acetate + L-asparagine
-
-
-
?
N-acetyl-L-Cys + H2O
acetate + L-Cys
-
-
-
-
?
N-acetyl-L-Cys + H2O
acetate + L-Cys
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
-
?
N-acetyl-L-ethionine + H2O
acetate + L-ethionine
-
-
-
?
N-acetyl-L-ethionine + H2O
acetate + L-ethionine
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
?
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
-
-
-
?
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
-
-
-
?
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
-
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
immobilised enzyme
-
-
?
N-acetyl-L-Met + H2O
acetate + L-Met
-
-
-
-
?
N-acetyl-L-Met + H2O
acetate + L-Met
highest activity
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
288852, 288853, 288854, 288856, 288857, 288859, 288860, 288862, 288863, 288864, 288866, 288867, 288868, 288869, 288870, 288871, 288872, 288873, 288874 -
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
preferred substrate
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?, r
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
r
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
immobilised enzyme
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
40.5% of the activity with N-acetyl-L-phenylalanine
-
-
?
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
-
-
-
?
N-acetyl-L-norleucine + H2O
acetate + L-norleucine
-
-
-
?
N-acetyl-L-norleucine + H2O
acetate + L-norleucine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
the reaction is highly stereoselective
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
immobilised enzyme, no activity observed with N-acetyl-L-lysine
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
no activity with N-acetyl-D-phenylalanine
-
-
?
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
-
-
-
-
?
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
-
-
-
-
?
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
-
-
-
-
?
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
-
prefers D-isomer
-
-
?
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
-
-
-
-
?
N-acetyl-L-threonine + H2O
acetate + L-threonine
-
-
-
?
N-acetyl-L-threonine + H2O
acetate + L-threonine
-
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
-
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
weak activity
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
weak activity
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
-
-
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
-
-
-
-
?
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
-
immobilised enzyme
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
immobilised enzyme
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
-
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
-
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
favorite substrate
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-L-valine + H2O
L-valine + acetate
-
-
-
-
?
N-acetyl-S-benzyl-L-cysteine + H2O
acetate + S-benzyl-L-cysteine
-
-
-
?
N-acetyl-S-benzyl-L-cysteine + H2O
acetate + S-benzyl-L-cysteine
-
-
-
-
?
N-acetylglycine + H2O
acetate + glycine
weak activity
-
-
?
N-acetylglycine + H2O
acetate + glycine
weak activity
-
-
?
N-alpha-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
-
?
N-alpha-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
-
?
N-alpha-acetyl-L-aspartate + H2O
acetate + L-aspartate
-
-
-
-
?
N-alpha-acetyl-L-aspartate + H2O
acetate + L-aspartate
-
-
-
-
?
N-alpha-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
-
?
N-alpha-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
-
?
N-alpha-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
-
?
N-alpha-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
-
?
N-alpha-acetyl-L-lysine + H2O
acetate + L-lysine
-
-
-
-
?
N-alpha-acetyl-L-lysine + H2O
acetate + L-lysine
-
-
-
?
N-benzoyl-L-glutamate + H2O
benzoate + L-glutamate
-
-
-
?
N-benzoyl-L-glutamate + H2O
benzoate + L-glutamate
-
-
-
-
?
N-benzoyl-L-methionine + H2O
benzoate + L-methionine
-
-
-
?
N-benzoyl-L-methionine + H2O
benzoate + L-methionine
-
-
-
-
?
N-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
-
-
-
-
?
N-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
-
immobilised enzyme
-
-
?
N-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
-
735.7% of the activity with N-acetyl-L-phenylalanine
-
-
?
N-butanoyl-L-methionine + H2O
butanoate + L-methionine
-
-
-
?
N-butanoyl-L-methionine + H2O
butanoate + L-methionine
-
-
-
-
?
N-carbobenzyloxy-L-glutamine + H2O
?
-
-
-
-
?
N-carbobenzyloxy-L-glutamine + H2O
?
-
-
-
-
?
N-chloroacetyl-DL-alanine + H2O
chloroacetate + alanine
-
-
-
-
?
N-chloroacetyl-DL-alanine + H2O
chloroacetate + alanine
-
-
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
-
-
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
-
-
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
-
-
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
-
-
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
-
-
-
-
?
N-chloroacetyl-L-methionine + H2O
chloroacetate + L-methionine
-
-
-
-
?
N-chloroacetyl-L-methionine + H2O
chloroacetate + L-methionine
-
-
-
-
?
N-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
-
-
-
-
?
N-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
-
immobilised enzyme
-
-
?
N-chloroacetylglutamine-p-aminobenzoate + H2O
?
-
-
-
-
?
N-chloroacetylglutamine-p-aminobenzoate + H2O
?
-
-
-
-
?
N-chloroacetylglycine + H2O
chloroacetate + glycine
-
N-chloroacetylamino acids are better substrates than N-acetylamino acids
-
-
?
N-chloroacetylglycine + H2O
chloroacetate + glycine
-
-
-
-
?
N-chloroacetyltryptophan + H2O
chloroacetate + tryptophan
-
-
-
-
?
N-chloroacetyltryptophan + H2O
chloroacetate + tryptophan
-
-
-
-
?
N-chloroacetyltryptophan + H2O
chloroacetate + tryptophan
-
-
-
-
?
N-decanoyl-L-glutamine + H2O
decanoate + L-glutamine
-
-
-
-
?
N-decanoyl-L-glutamine + H2O
decanoate + L-glutamine
-
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
-
hydrolyzed at 60% rate of N-acetylmethionine
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
-
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
-
?
N-isobutanoyl-L-methionine + H2O
isobutanoate + L-methionine
-
-
-
?
N-isobutanoyl-L-methionine + H2O
isobutanoate + L-methionine
-
-
-
-
?
N-isopentanoyl-L-methionine + H2O
isopentanoate + L-methionine
-
-
-
?
N-isopentanoyl-L-methionine + H2O
isopentanoate + L-methionine
-
-
-
-
?
N-lauroyl-L-glutamine + H2O
laurate + L-glutamine
-
-
-
-
?
N-lauroyl-L-glutamine + H2O
laurate + L-glutamine
-
-
-
-
?
N-lauroyl-L-glutamine + H2O
laurate + L-glutamine
-
-
-
-
?
N-pentanoyl-L-methionine + H2O
pentanoate + L-methionine
-
-
-
?
N-pentanoyl-L-methionine + H2O
pentanoate + L-methionine
-
-
-
-
?
N-propionyl-L-methionine + H2O
propionate + L-methionine
-
-
-
?
N-propionyl-L-methionine + H2O
propionate + L-methionine
-
-
-
-
?
additional information
?
-
-
butanolysis and enantioselective hydrolysis of carboxylic acid esters, the activity of the enzyme is not restricted to N-acyl transfers, other substrates are e.g. L-aminobutyric acid alkyl esters, L-aminobutyric acid amide, L-4-hydroxyphenylglycine, and L-4-hydroxyphenylglycine amide, overview
-
-
?
additional information
?
-
-
aminoacylase catalyzes the hydrolysis of L-acyl-amino acids to produce the corresponding L-amino acid
-
-
?
additional information
?
-
-
N-acyl-D-alanine is not substrate
-
-
?
additional information
?
-
-
N-acyl-D-alanine is not substrate
-
-
?
additional information
?
-
-
the enzyme is involved in the regulation of oxidative stress, and, by direct interaction, of sphingosine kinase type 1, SphK1, which has a role in cell growth promotion and inhibition of tumor cell apoptosis, Acy1 induces redistribution of SphK1 to the plasma membrane from cytosol, overview
-
-
?
additional information
?
-
-
the enzyme interacts with sphingosine kinase type 1, SphK1
-
-
?
additional information
?
-
-
AA1 similar to AA3 is involved in deacetylation of N-acetyl-aromatic amino acids and mercapturic acids
-
-
?
additional information
?
-
-
AA3 residues Arg63, Asp68, Asn70, Arg71, Glu177 and Tyr287 are potentially involved in catalysis/substrate binding
-
-
?
additional information
?
-
N-acetyl-D-amino acids are not hydrolyzed
-
-
?
additional information
?
-
-
N-acetyl-D-amino acids are not hydrolyzed
-
-
?
additional information
?
-
N-acetyl-D-amino acids are not hydrolyzed
-
-
?
additional information
?
-
-
the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1
-
-
?
additional information
?
-
-
no activity with N-formylmethionylalanine, N-formylalanylalanine, N-formylmethionylalanylserine, N-acetylmethionylalanylserine, and N-formylmethionylleucylphenylalanine
-
-
?
additional information
?
-
-
substrate synthesis in an aqueous/n-hexane biphasic system
-
-
?
additional information
?
-
the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc
-
-
?
additional information
?
-
-
the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc
-
-
?
additional information
?
-
no activity towards N-acetyl-L-Lys, N-acetyl-L-Glu, N-acetyl-L-Pro, N-acetyl-L-Trp, N-acetyl-L-Tyr, N-lauroyl-L-Ile, N-lauroyl-L-Leu, N-lauroyl-L-Phe, N-lauroyl-L-Pro, N-lauroyl-L-Trp, N-lauroyl-L-Tyr, and N-lauroyl-L-Val
-
-
?
additional information
?
-
-
no activity towards N-acetyl-L-Lys, N-acetyl-L-Glu, N-acetyl-L-Pro, N-acetyl-L-Trp, N-acetyl-L-Tyr, N-lauroyl-L-Ile, N-lauroyl-L-Leu, N-lauroyl-L-Phe, N-lauroyl-L-Pro, N-lauroyl-L-Trp, N-lauroyl-L-Tyr, and N-lauroyl-L-Val
-
-
?
additional information
?
-
the enzyme hydrolyzes N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids. Poor or no activity with N-acetyl-amino acids containing L-Lys, L-Asp, L-Cys, L-Pro, L-Trp, and L-Tyr, as well as with N-leuroyl-amino acids containing L-Arg, L-His, L-Asn, L-Ile, L-Leu, L-Phe, L-Pro, L-Trp, L-Tyr, and L-Val, substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme hydrolyzes N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids. Poor or no activity with N-acetyl-amino acids containing L-Lys, L-Asp, L-Cys, L-Pro, L-Trp, and L-Tyr, as well as with N-leuroyl-amino acids containing L-Arg, L-His, L-Asn, L-Ile, L-Leu, L-Phe, L-Pro, L-Trp, L-Tyr, and L-Val, substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme cataylzes high enantioselective reactions
-
-
?
additional information
?
-
pAcy1 catalyzes the highly stereoselective acylation of amino acids, a useful conversion for the preparation of optically pure N-acyl-L-amino acids, the catalytic base is E146. pAcy1 from pig kidney displays a marked preference for short-chain acyl moieties and non-branched aliphatic L-amino acids. Modeling of pAcy1 catalyzed N-acylation, overview
-
-
?
additional information
?
-
-
pAcy1 catalyzes the highly stereoselective acylation of amino acids, a useful conversion for the preparation of optically pure N-acyl-L-amino acids, the catalytic base is E146. pAcy1 from pig kidney displays a marked preference for short-chain acyl moieties and non-branched aliphatic L-amino acids. Modeling of pAcy1 catalyzed N-acylation, overview
-
-
?
additional information
?
-
-
the ability of the enzyme to discriminate between methyls and fluorinated methyls is limited. For example, there is no enantioselectivity in the enzymatic hydrolysis of N-trifluoroacetyl 2-fluoromethylalanine
-
-
-
additional information
?
-
-
the enzyme is most specific for substrates containing N-benzoyl- or N-chloroacetyl-amino acids, preferring substrates containing hydrophobic, uncharged, or weakly charged amino acids such as phenylalanine, methionine, and cysteine. It contains esterase activity with 4-nitrophenyl-palmitate and 4-nitrophenyllaurate, but not Phe-ethyl ester at 85°C
-
-
?
additional information
?
-
-
the enzyme is most specific for substrates containing N-benzoyl- or N-chloroacetyl-amino acids, preferring substrates containing hydrophobic, uncharged, or weakly charged amino acids such as phenylalanine, methionine, and cysteine. No activity with N-benzoyl-D-phenylalanine, N-benzyloxycarbonyl-D-phenylalanine, tert-butyloxycarbonyl-D-phenylalanine, N-acetyl-D-tryptophan
-
-
?
additional information
additional information
-
-
the order of preferred substrates is Phe > Thr > Leu > Arg for N-benzoyl substrates and Phe >> Ser > Leu > Met > Tyr > Trp for N-acetyl substrates
-
-
?
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0.1 - 0.2
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
0.3 - 0.5
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
1
2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
0.2 - 0.4
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
0.22
furylacryloyl-L-methionine
-
-
0.2
N-(E)-3-methyl-2-hexenoyl-L-glutamine
-
pH 7
168 - 585
N-2,2-dimethylpropionyl-L-methionine
0.74
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine
-
pH 7
2.52
N-acetyl-D-aspartate
-
-
12.7
N-acetyl-DL-aspartate
-
-
43.6 - 59.9
N-acetyl-glycine
2.32 - 20.1
N-acetyl-L-alanine
34.8
N-acetyl-L-arginine
25°C, pH 7.4
27.7
N-acetyl-L-asparagine
25°C, pH7.4
1.4 - 4.4
N-acetyl-L-cysteine
1.28 - 1.87
N-acetyl-L-ethionine
63 - 102
N-acetyl-L-glutamate
5.9
N-acetyl-L-glutamic acid
-
pH 7.0, 30°C
7 - 62.3
N-acetyl-L-glutamine
1.8 - 22.7
N-acetyl-L-histidine
6.72
N-acetyl-L-isoleucine
25°C, pH 7.4
2.4 - 3.8
N-acetyl-L-leucine
0.2 - 11.3
N-acetyl-L-Met
0.013 - 24.6
N-acetyl-L-methionine
0.44 - 1.57
N-acetyl-L-norleucine
1.6 - 10.3
N-acetyl-L-phenylalanine
30.9 - 32.5
N-acetyl-L-threonine
1.2
N-acetyl-L-tryptophan
37°C, pH 7.5
1.4
N-acetyl-L-tyrosine
37°C, pH 7.5
2.5 - 16.6
N-acetyl-L-valine
0.25
N-acetyl-S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
37°C, pH 7.5
1.2
N-acetyl-S-(1,2,2-trichlorovinyl)-L-cysteine
37°C, pH 7.5
1
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine
37°C, pH 7.5
0.9
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine
37°C, pH 7.5
5.3
N-acetyl-S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine
37°C, pH 7.5
0.4
N-acetyl-S-(2,2-dichlorovinyl)-L-cysteine
37°C, pH 7.5
0.3
N-acetyl-S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine
37°C, pH 7.5
0.28
N-acetyl-S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
37°C, pH 7.5
1.45
N-acetyl-S-(2-chlorobenzyl)-L-cysteine
37°C, pH 7.5
1.3
N-acetyl-S-(2-fluorobenzyl)-L-cysteine
37°C, pH 7.5
0.6
N-acetyl-S-(3-fluorobenzyl)-L-cysteine
37°C, pH 7.5
0.5
N-acetyl-S-(4-bromobenzyl)-L-cysteine
37°C, pH 7.5
0.3
N-acetyl-S-(4-chlorobenzyl)-L-cysteine
37°C, pH 7.5
0.8
N-acetyl-S-(4-methoxybenzyl)-L-cysteine
37°C, pH 7.5
1.1
N-acetyl-S-benzyl-L-cysteine
37°C, pH 7.5
0.3
N-acetylmethionine
-
pH 8.0, 22°C
4
N-acetylseleno-L-methionine
-
-
1.2
N-alpha-acetyl-L-alanine
-
pH 7.0, 30°C
5
N-alpha-acetyl-L-aspartate
-
pH 7.0, 30°C
9.1
N-alpha-acetyl-L-cysteine
-
pH 7.0, 30°C
3.1
N-alpha-acetyl-L-isoleucine
-
pH 7.0, 30°C
2.3
N-alpha-acetyl-L-leucine
-
pH 7.0, 30°C
1.3 - 6.7
N-alpha-acetyl-L-lysine
0.84 - 7.7
N-alpha-acetyl-L-methionine
1.7 - 2.7
N-alpha-acetyl-L-phenylalanine
5
N-alpha-acetyl-L-proline
-
pH 7.0, 30°C
4.2
N-alpha-acetyl-L-serine
-
pH 7.0, 30°C
4
N-alpha-acetyl-L-threonine
-
pH 7.0, 30°C
2.4
N-alpha-acetyl-L-tryptophan
-
pH 7.0, 30°C
1.9
N-alpha-acetyl-L-tyrosine
-
pH 7.0, 30°C
2.5
N-alpha-acetyl-L-valine
-
pH 7.0, 30°C
1.4
N-alpha-chloroacetyl-L-phenylalanine
-
pH 7.0, 30°C
3.71
N-benzoyl-L-glutamate
-
hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1
0.38 - 1.63
N-benzoyl-L-methionine
4 - 9.7
N-benzoyl-L-phenylalanine
5 - 10.7
N-benzoyl-L-valine
0.31 - 0.73
N-butanoyl-L-methionine
0.033
N-butyryl-D-aspartate
-
-
0.31 - 6.23
N-cyclohexanoyl-L-methionine
0.64 - 7.18
N-cyclopentanoyl-L-methionine
0.08
N-decanoyl-L-glutamine
-
pH 7
12.5
N-formyl-D-aspartate
-
-
16.8
N-formyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
35.4
N-formyl-Lmethionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
3.1
N-formylmethionine
-
pH 8.0, 22°C
1.11
N-glycyl-D-aspartate
-
-
0.01 - 5.22
N-hexanoyl-L-methionine
15.3 - 58.5
N-isobutanoyl-L-methionine
0.54 - 7.29
N-isopentanoyl-L-methionine
0.06
N-lauroyl-L-glutamine
-
pH 7
0.03 - 0.19
N-pentanoyl-L-methionine
0.194
N-propionyl-D-aspartate
-
-
0.34 - 0.55
N-propionyl-L-methionine
0.05
N2-[(benzyloxy)carbonyl]-L-glutamine
-
pH 7
0.39 - 67.4
Nalpha-acetylmethionine
0.15
palmitoylaspartate
-
-
3.4
S-ethyl-N-acetyl-L-cysteine
-
-
2.1
S-methyl-N-acetyl-L-cysteine
-
-
3.7
S-n-butyl-N-acetyl-L-cysteine
-
-
0.8
S-n-propyl-N-acetyl-L-cysteine
-
-
additional information
additional information
-
0.1
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
0.15
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
0.2
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.3
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
0.5
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.5
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
0.2
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
0.3
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.4
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by HPLC, pH 7.6, 37°C
168
N-2,2-dimethylpropionyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
585
N-2,2-dimethylpropionyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
43.6
N-acetyl-glycine
25°C, pH 7.4
59.9
N-acetyl-glycine
25°C, pH7.4
2.32
N-acetyl-L-alanine
-
-
20.1
N-acetyl-L-alanine
25°C, pH7.4
0.4
N-acetyl-L-Cys
analysis by HPLC, pH 7.6, 37°C
0.5
N-acetyl-L-Cys
-
analysis by HPLC, pH 7.6, 37°C
0.5
N-acetyl-L-Cys
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
1.4
N-acetyl-L-cysteine
25°C, pH7.4
1.5
N-acetyl-L-cysteine
25°C, pH 7.4
4.4
N-acetyl-L-cysteine
-
-
1.28
N-acetyl-L-ethionine
25°C, pH7.4
1.87
N-acetyl-L-ethionine
25°C, pH 7.4
63
N-acetyl-L-glutamate
25°C, pH7.4
63
N-acetyl-L-glutamate
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
70
N-acetyl-L-glutamate
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
102
N-acetyl-L-glutamate
25°C, pH 7.4
102
N-acetyl-L-glutamate
-
hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1
7
N-acetyl-L-glutamine
-
hydrolysis in D2O, MOPS/NaOH buffer
10.2
N-acetyl-L-glutamine
-
MOPS/NaOH buffer
57.6
N-acetyl-L-glutamine
25°C, pH 7.4
62.3
N-acetyl-L-glutamine
25°C, pH7.4
1.8
N-acetyl-L-histidine
37°C, pH 7.5
11.9
N-acetyl-L-histidine
25°C, pH 7.4
22.7
N-acetyl-L-histidine
25°C, pH7.4
2.4
N-acetyl-L-leucine
25°C, pH7.4
3.8
N-acetyl-L-leucine
25°C, pH 7.4
0.2
N-acetyl-L-Met
analysis by HPLC, pH 7.6, 37°C
0.3
N-acetyl-L-Met
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
0.5
N-acetyl-L-Met
-
analysis by HPLC, pH 7.6, 37°C
11.3
N-acetyl-L-Met
recombinant enzyme, at 37°C, pH not specified in the publication
0.013
N-acetyl-L-methionine
-
addition of 0.2 mM Co2+
0.056
N-acetyl-L-methionine
-
addition of 0.2 mM Mn2+
0.4
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.43
N-acetyl-L-methionine
25°C, pH7.4
0.43
N-acetyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.76
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant
0.964
N-acetyl-L-methionine
-
pH 8.0, 40°C, native enzyme
0.99
N-acetyl-L-methionine
-
-
1
N-acetyl-L-methionine
-
-
1.14
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
1.35
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
1.4
N-acetyl-L-methionine
-
immobilized enzyme
1.497
N-acetyl-L-methionine
-
pH 8.0, 40°C, immobilized, cross-linked enzyme-bovine serum albumin aggregate
1.62
N-acetyl-L-methionine
-
ordinary water, 50 mM MOPS 50 mM bicine buffer
1.72
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
1.87
N-acetyl-L-methionine
-
hydrolysis in D2O, 50 mM MOPS 50 mM bicine buffer
2.1
N-acetyl-L-methionine
-
-
2.72
N-acetyl-L-methionine
25°C, pH 7.4
2.72
N-acetyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
2.76
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
2.78
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant
4.18
N-acetyl-L-methionine
pH 7.5, 85°C, wild-type enzyme
4.32
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant
5
N-acetyl-L-methionine
-
6
N-acetyl-L-methionine
-
soluble enzyme
24.6
N-acetyl-L-methionine
-
0.44
N-acetyl-L-norleucine
25°C, pH7.4
1.57
N-acetyl-L-norleucine
25°C, pH 7.4
1.6
N-acetyl-L-phenylalanine
37°C, pH 7.5
4.81
N-acetyl-L-phenylalanine
25°C, pH7.4
5.2
N-acetyl-L-phenylalanine
pH 7.5, 85°C, wild-type enzyme
5.5
N-acetyl-L-phenylalanine
-
ordinary water, MOPS/NaOH buffer
5.6
N-acetyl-L-phenylalanine
-
hydrolysis in D2O, MOPS/NaOH buffer, distinctly inverse deuterium isotope effect
6.2
N-acetyl-L-phenylalanine
pH 7.5, 85°C, mutant enzyme C102S
6.64
N-acetyl-L-phenylalanine
pH 7.5, 85°C, mutant enzyme E367Q
10.3
N-acetyl-L-phenylalanine
25°C, pH 7.4
30.9
N-acetyl-L-threonine
25°C, pH 7.4
32.5
N-acetyl-L-threonine
25°C, pH7.4
0.4
N-acetyl-L-Val
analysis by HPLC, pH 7.6, 37°C
0.5
N-acetyl-L-Val
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
2.5
N-acetyl-L-valine
-
-
11.4
N-acetyl-L-valine
25°C, pH7.4
16.6
N-acetyl-L-valine
25°C, pH 7.4
1.3
N-alpha-acetyl-L-lysine
37°C, pH 7.5
6.7
N-alpha-acetyl-L-lysine
-
pH 7.0, 30°C
0.84
N-alpha-acetyl-L-methionine
native porcine enzyme,25°C
1.2
N-alpha-acetyl-L-methionine
E309H-mutant, 25°C
1.25
N-alpha-acetyl-L-methionine
Wild type Rosetta (DE3),25°C
2.12
N-alpha-acetyl-L-methionine
R274A-mutant, 25°C
2.21
N-alpha-acetyl-L-methionine
H79A-mutant, 25°C
2.23
N-alpha-acetyl-L-methionine
E147A-mutant, 25°C
2.36
N-alpha-acetyl-L-methionine
E174A-mutant, 25°C
2.45
N-alpha-acetyl-L-methionine
H371A-mutant, 25°C
2.52
N-alpha-acetyl-L-methionine
D112A-mutant, 25°C
3.82
N-alpha-acetyl-L-methionine
H205L-mutant, 25°C
4.34
N-alpha-acetyl-L-methionine
E146A-mutant, 25°C
4.57
N-alpha-acetyl-L-methionine
N261A-mutant, 25°C
7.7
N-alpha-acetyl-L-methionine
-
pH 7.0, 30°C
1.7
N-alpha-acetyl-L-phenylalanine
-
pH 7.0, 30°C
2.7
N-alpha-acetyl-L-phenylalanine
-
pH 7.0, 30°C
0.38
N-benzoyl-L-methionine
-
hydrolysis of N-acetyl-L-methionine substrates by Wild-Type porcine Acy1
0.41
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1
0.81
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1
1.63
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
4
N-benzoyl-L-phenylalanine
-
S100T/M106K mutant protein, pH 8.0, 55°C
8.5
N-benzoyl-L-phenylalanine
-
wild type protein, pH 8.0, 55°C
9.7
N-benzoyl-L-phenylalanine
-
F251K mutant protein, pH 8.0, 55°C
5
N-benzoyl-L-valine
-
F251K mutant protein, pH 8.0, 55°C
6.7
N-benzoyl-L-valine
-
wild type protein, pH 8.0, 55°C
10.7
N-benzoyl-L-valine
-
S100T/M106K mutant protein, pH 8.0, 55°C
0.31
N-butanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
0.73
N-butanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.31
N-cyclohexanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
1.17
N-cyclohexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
6.23
N-cyclohexanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.64
N-cyclopentanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
7.18
N-cyclopentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.01
N-hexanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
1.01
N-hexanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
3.56
N-hexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
5.22
N-hexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
15.3
N-isobutanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
58.5
N-isobutanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
0.54
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
0.9
N-isopentanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
2.1
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
4.27
N-isopentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
7.29
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
0.03
N-pentanoyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
0.19
N-pentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.34
N-propionyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.55
N-propionyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
0.39
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant D274A
0.43
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.54
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206A
0.58
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206K
0.78
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant N263D
2.02
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206N
67.4
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant R276A
additional information
additional information
-
steady-state kinetics of wild-type and mutant enzymes, kinetics of mutants N263D, R276N, and R276Q are not measurable
-
additional information
additional information
-
kinetics of wild-type AA3 in presnece of different metal ions, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45 - 150
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
26 - 120
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
3
2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
19.5 - 80
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
0.0015
N-2,2-dimethylpropionyl-L-methionine
7.6 - 31.7
N-acetyl-glycine
0.04 - 8.53
N-acetyl-L-arginine
0.1
N-acetyl-L-aspartate
-
E167R mutant protein, pH not specified in the publication, temperature not specified in the publication
28.1 - 34.7
N-acetyl-L-cysteine
12.4 - 68.3
N-acetyl-L-ethionine
63.4 - 207
N-acetyl-L-glutamate
30.7 - 146
N-acetyl-L-glutamine
1.2 - 2.77
N-acetyl-L-histidine
1.28
N-acetyl-L-isoleucine
25°C, pH 7.4
55.5 - 160
N-acetyl-L-leucine
1.2 - 370
N-acetyl-L-methionine
156 - 266
N-acetyl-L-norleucine
0.0208 - 37.3
N-acetyl-L-phenylalanine
1.21 - 2.23
N-acetyl-L-threonine
0.6
N-acetyl-L-tyrosine
-
E167R mutant protein, pH not specified in the publication, temperature not specified in the publication
11.9 - 14.7
N-acetyl-L-valine
2.3
N-acetylmethionine
-
pH 8.0, 22°C
0.0101 - 0.115
N-benzoyl-L-glutamate
0.158 - 20.1
N-benzoyl-L-methionine
3620 - 21300
N-benzoyl-L-phenylalanine
98.1 - 9380
N-benzoyl-L-valine
107 - 139
N-butanoyl-L-methionine
2 - 8
N-Cbz-Gly-L-Ala
-
-
-
0.00623 - 0.145
N-cyclohexanoyl-L-methionine
11.2 - 13.3
N-cyclopentanoyl-L-methionine
177
N-formyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
200
N-formyl-Lmethionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
7.9
N-formylmethionine
-
pH 8.0, 22°C
0.052 - 39.4
N-hexanoyl-L-methionine
0.529 - 89.8
N-isobutanoyl-L-methionine
0.105 - 6.08
N-isopentanoyl-L-methionine
16.2 - 18.2
N-pentanoyl-L-methionine
25.7 - 168
N-propionyl-L-methionine
0.006 - 38.3
Nalpha-acetylmethionine
additional information
additional information
-
kinetics of mutants N263D, R276N, and R276Q are not measurable
-
45
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
50
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
150
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
26
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
30
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
120
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
19.5
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by HPLC, pH 7.6, 37°C
21
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
80
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.0015
N-2,2-dimethylpropionyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.0015
N-2,2-dimethylpropionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
7.6
N-acetyl-glycine
25°C, pH 7.4
31.7
N-acetyl-glycine
25°C, pH7.4
0.04 - 1.97
N-acetyl-L-arginine
25°C, pH 7.4
8.53
N-acetyl-L-arginine
25°C, pH 7.4
9
N-acetyl-L-Cys
analysis by HPLC, pH 7.6, 37°C
18
N-acetyl-L-Cys
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
30
N-acetyl-L-Cys
-
analysis by HPLC, pH 7.6, 37°C
28.1
N-acetyl-L-cysteine
25°C, pH7.4
34.7
N-acetyl-L-cysteine
25°C, pH 7.4
12.4
N-acetyl-L-ethionine
25°C, pH7.4
68.3
N-acetyl-L-ethionine
25°C, pH 7.4
63.4
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
120
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
156
N-acetyl-L-glutamate
25°C, pH7.4
156
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
207
N-acetyl-L-glutamate
25°C, pH 7.4
207
N-acetyl-L-glutamate
-
kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1
30.7
N-acetyl-L-glutamine
25°C, pH7.4
146
N-acetyl-L-glutamine
25°C, pH 7.4
1.2
N-acetyl-L-histidine
25°C, pH7.4
2.77
N-acetyl-L-histidine
25°C, pH 7.4
55.5
N-acetyl-L-leucine
25°C, pH7.4
160
N-acetyl-L-leucine
25°C, pH 7.4
19
N-acetyl-L-Met
analysis by HPLC, pH 7.6, 37°C
30
N-acetyl-L-Met
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
65
N-acetyl-L-Met
-
analysis by HPLC, pH 7.6, 37°C
1.2
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant
4.49
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
12.1
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
16.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant
38.3
N-acetyl-L-methionine
25°C, pH7.4
38.3
N-acetyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
45.6
N-acetyl-L-methionine
pH 7.5, 85°C, wild-type enzyme
46
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant
64.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
66.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
93.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
134
N-acetyl-L-methionine
25°C, pH 7.4
134
N-acetyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
370
N-acetyl-L-methionine
-
156
N-acetyl-L-norleucine
25°C, pH7.4
266
N-acetyl-L-norleucine
25°C, pH 7.4
0.0208
N-acetyl-L-phenylalanine
pH 7.5, 85°C, mutant enzyme C102S
0.14
N-acetyl-L-phenylalanine
25°C, pH7.4
0.81
N-acetyl-L-phenylalanine
25°C, pH 7.4
34
N-acetyl-L-phenylalanine
pH 7.5, 85°C, wild-type enzyme
37.3
N-acetyl-L-phenylalanine
pH 7.5, 85°C, mutant enzyme E367Q
1.21
N-acetyl-L-threonine
25°C, pH 7.4
2.23
N-acetyl-L-threonine
25°C, pH7.4
1.7
N-acetyl-L-Val
analysis by HPLC, pH 7.6, 37°C
2
N-acetyl-L-Val
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
11.9
N-acetyl-L-valine
25°C, pH 7.4
14.7
N-acetyl-L-valine
25°C, pH7.4
0.0101
N-benzoyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
0.115
N-benzoyl-L-glutamate
-
kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1
0.158
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
1.3
N-benzoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
20.1
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1-T347S-mutant
3620
N-benzoyl-L-phenylalanine
-
S100T/M106K mutant protein, pH 8.0, 55°C
5900
N-benzoyl-L-phenylalanine
-
F251K mutant protein, pH 8.0, 55°C
21300
N-benzoyl-L-phenylalanine
-
wild type protein, pH 8.0, 55°C
98.1
N-benzoyl-L-valine
-
F251K mutant protein, pH 8.0, 55°C
1760
N-benzoyl-L-valine
-
wild type protein, pH 8.0, 55°C
9380
N-benzoyl-L-valine
-
S100T/M106K mutant protein, pH 8.0, 55°C
107
N-butanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
139
N-butanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.00623
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.129
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.145
N-cyclohexanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
11.2
N-cyclopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
13.3
N-cyclopentanoyl-L-methionine
kcat the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.052 - 2.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
4.3
N-hexanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
9.51
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
16.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
39.4
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
0.529
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
6.08
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
89.8
N-isobutanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.105
N-isopentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.34
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
0.694
N-isopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
2.01
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
3.3
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
5.36
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
6.08
N-isopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
16.2
N-pentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
18.2
N-pentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
25.7
N-propionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
168
N-propionyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.006
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant D274A
0.015
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206N
0.086
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206A
0.149
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206K
0.458
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant N263D
0.667
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant R276A
38.3
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant wild-type enzyme
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0.00000028
substrate N-linolenoyl-L-glutamine, protein partially purified from frass, pH 8.0, 20°C
0.0006
-
S100T/M106K mutant protein, substrate N-acetylpipecolic acid, pH 8.0, 55°C
0.0042
-
transformant 1 with plasmid pSACY2
0.0045
-
transformant 2 with plasmid pSACY2
0.005
-
F251K mutant protein, substrate N-benzoyl-L-isoleucine, pH 8.0, 55°C
0.008
-
F251K mutant protein, substrate N-benzoyl-L-tert-leucine, pH 8.0, 55°C
0.0092
-
pH 8.0, temperature not specified in the publication, substrate: acetyl-DL-Trp
0.012
-
transformant 1 with pMACY2
0.02
-
transformant 2 with pMACY2
0.024
-
wild type protein, substrate N-acetylpipecolic acid, pH 8.0, 55°C
0.025
-
transformant 3 with pMACY2
0.026
-
S100T/M106K mutant protein, substrate N-benzoyl-L-isoleucine, pH 8.0, 55°C
0.075
-
wild type protein, substrate N-benzoyl-L-tert-leucine, pH 8.0, 55°C
0.112
-
F251K mutant protein, substrate N-benzoyl-L-valine, pH 8.0, 55°C
0.12
-
N-acetyl-D-Phe 8 mM
0.19
-
wild type protein, substrate N-benzoyl-L-isoleucine, pH 8.0, 55°C
0.37
-
N-CBZ-Ile-Pro 6 mM
1.16
-
N-CBZ-Ala-Glu 6 mM
1.256
-
F251K mutant protein, substrate N-acetyl-L-phenylalanine, pH 8.0, 55°C
1.405
-
S100T/M106K mutant protein, substrate N-acetyl-L-phenylalanine, pH 8.0, 55°C
1.55
-
S100T/M106K mutant protein, substrate N-benzoyl-L-phenylalanine, pH 8.0, 55°C
1.679
-
wild type protein, substrate N-benzoyl-L-valine, pH 8.0, 55°C
1.74
-
N-acetyl-L-homophenylalanine 8 mM
10.3
Wild type BL21 (DE3), 25°C
10.7
-
N-CBZ-Gly-Tyr 6 mM
105
using 3 mM N-palmitoyl-L-Met as substrate, at 37°C, pH not specified in the publication
106
using 3 mM N-myristoyl-L-Met as substrate, at 37°C, pH not specified in the publication
11.26
-
N-acetyl-L-His 8 mM
11.7
activity of the tetramer toward S-benzyl-N-acetyl-L-cysteine
110
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine
118
-
purified enzyme, substrate N-lauroyl-L-histidine
129
-
purified enzyme, substrate N-lauroyl-L-leucine
138
-
purified enzyme, substrate N-lauroyl-L-aspartic acid
145
-
purified enzyme, substrate N-lauroyl-L-tyrosine
15.1
-
N-CBZ-Gly-Ala 6 mM
169
using 3 mM N-butyryl-L-Met as substrate, at 37°C, pH not specified in the publication
17
using N-lauroyl-L-Cys as substrate, at 37°C, pH not specified in the publication
173
using 3 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication
175
using 3 mM N-hexanoyl-L-Met as substrate, at 37°C, pH not specified in the publication
177
-
purified enzyme, substrate N-lauroyl-L-methionine
19
using N-acetyl-L-Phe as substrate, at 37°C, pH not specified in the publication
196
-
purified enzyme, substrate N-lauroyl-L-glutamic acid
2.15
-
F251K mutant protein, substrate N-benzoyl-L-phenylalanine, pH 8.0, 55°C
2.52
-
N-acetyl-L-Leu 8 mM
2.9
recombinant wild-type enzyme, substrate L-methionine, synthesis reaction, pH 6.0, 25°C
210
-
purified enzyme, substrate capsaicin
22
-
purified enzyme, substrate N-lauroyl-L-tryptophan
220
Wild type Rosetta (DE3), 25°C
221
-
purified enzyme, substrate N-lauroyl-L-glutamine
222
-
purified enzyme, substrate N-lauroyl-glycine
227
-
purified enzyme, substrate N-lauroyl-L-asparagine
230
-
purified enzyme, substrate N-lauroyl-L-threonine
236
-
purified enzyme, substrate N-lauroyl-L-alanine
24
using N-acetyl-L-Val as substrate, at 37°C, pH not specified in the publication
244
-
purified enzyme, substrate N-lauroyl-L-lysine
25
-
purified enzyme, substrate N-lauroyl-L-phenylalanine
251
using 3 mM N-octanoyl-L-Met as substrate, at 37°C, pH not specified in the publication
287
native porcine enzyme, 25°C
295
-
purified enzyme, substrate N-lauroyl-L-serine
3.32
-
S100T/M106K mutant protein, substrate N-benzoyl-L-valine, pH 8.0, 55°C
31
using N-acetyl-L-Asn as substrate, at 37°C, pH not specified in the publication
34
using N-lauroyl-Gly as substrate, at 37°C, pH not specified in the publication
36
using N-lauroyl-L-Ser as substrate, at 37°C, pH not specified in the publication
4
using N-lauroyl-L-Arg as substrate, at 37°C, pH not specified in the publication
440
purified native wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C
5.27
-
N-CBZ-Gly-Gly-Phe 6 mM
5.555
-
wild type protein, substrate N-acetyl-L-phenylalanine, pH 8.0, 55°C
59
using N-acetyl-L-His as substrate, at 37°C, pH not specified in the publication
6.8
-
wild type protein, substrate N-benzoyl-L-phenylalanine, pH 8.0, 55°C
6.84
-
N-chloroacetyl-L-Phe 8 mM
60
using N-acetyl-L-Leu as substrate, at 37°C, pH not specified in the publication
66
using N-acetyl-L-Arg as substrate, at 37°C, pH not specified in the publication
70
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine
72
using 3 mM N-lauroyl-L-Met as substrate, at 37°C, pH not specified in the publication
77
recombinant wild-type enzyme, substrate N-acetyl-L-methionine, hydrolytic reaction, pH 6.0, 25°C
8.07
-
N-CBZ-Gly-Leu 6 mM
8.4
-
N-acetyl-L-Phe 8 mM
8.54
-
N-CBZ-Ala-Met 6 mM
87
-
purified enzyme, substrate N-lauroyl-L-valine
89
using 3 mM N-decanoyl-L-Met as substrate, at 37°C, pH not specified in the publication
0.003
-
F251K mutant protein, substrate N-acetylpipecolic acid, pH 8.0, 55°C
0.003
-
S100T/M106K mutant protein, substrate N-benzoyl-L-tert-leucine, pH 8.0, 55°C
0.55
-
for DR_ACY/CP
0.55
-
N-acetyl-L-Trp 8 mM
0.89
native wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C
0.89
recombinant wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C
14
using N-acetyl-L-Gln as substrate, at 37°C, pH not specified in the publication
14
using N-lauroyl-L-Lys as substrate, at 37°C, pH not specified in the publication
194
-
purified enzyme, substrate N-lauroyl-L-arginine
194
using N-acetyl-L-Ala as substrate, at 37°C, pH not specified in the publication
412
purified recombinant wild-type enzyme, substrate N-acetyl-L-cysteine, pH 7.5, 37°C
412
using N-acetyl-L-Cys as substrate, at 37°C, pH not specified in the publication
5.73
-
for ACY
5.73
-
N-acetyl-L-Met 8 mM
530
purified recombinant wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C
530
using 15 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication
6
using N-lauroyl-L-Asn as substrate, at 37°C, pH not specified in the publication
6
using N-lauroyl-L-His as substrate, at 37°C, pH not specified in the publication
8
using N-acetyl-Gly as substrate, at 37°C, pH not specified in the publication
8
using N-acetyl-L-Asp as substrate, at 37°C, pH not specified in the publication
9.4
activity of the dimer toward S-benzyl-N-acetyl-L-cysteine
90
-
purified enzyme, substrate N-lauroyl-L-glutamyl-glycine
90
-
purified enzyme, substrate N-lauroyl-L-isoleucine
96
purified recombinant wild-type enzyme, substrate N-lauryl-L-alanine, pH 7.5, 37°C
96
using N-lauroyl-L-Ala as substrate, at 37°C, pH not specified in the publication
additional information
-
1.74 U/mg in phosphate buffer and 0.88 U/mg in 0.1M Tris-HCl buffer
additional information
-
-
additional information
substrate specificities of recombinant and native enzyme, overview
additional information
-
substrate specificities of recombinant and native enzyme, overview
additional information
-
maximum specific activity 22.25-27.75 U/ml at 40ºC and pH 7
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Moriguchi, M.; Sakai, K.; Katsuno, Y.; Maki, T.; Wakayama, M.
Purification and characterization of novel N-acyl-D-aspartate amidohydrolase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6
Biosci. Biotechnol. Biochem.
57
1145-1148
1993
Alcaligenes sp.
brenda
Birnbaum, S.M.; Levintow, L.; Kingsley, R.B.; Greenstein, J.P.
Specificity of amino acid acylases
J. Biol. Chem.
194
455-470
1952
Sus scrofa
brenda
Fones, W.S.; Lee, M.
Hydrolysis of N-acyl derivatives of alanine and phenylalanine by acylase I and carboxypeptidase
J. Biol. Chem.
201
847-856
1953
Sus scrofa
brenda
Park, R.W.; Fox, S.W.
An acylase system related to the utilization of benzoylamino acids by Lactobacillus arabinosus
J. Biol. Chem.
235
3193-3197
1960
Streptomyces sp., Lactiplantibacillus plantarum, Sus scrofa
brenda
Matsumoto, J.; Nagai, S.
Amidohydrolases for N-short and long chain fatty acyl-L-amino acids from Mycobacteria
J. Biochem.
72
269-279
1972
Mycobacterium sp.
brenda
Koerdel, W.; Schneider, F.
Chemical investigations on pig kidney aminoacylase
Biochim. Biophys. Acta
445
446-457
1976
Sus scrofa
brenda
Gentzen, I.; Lffler, H.G.; Schneider, F.
Aminoacylase from Aspergillus oryzae. Comparison with the pig kidney enzyme
Z. Naturforsch. C
35
544-550
1980
Aspergillus oryzae, Sus scrofa
brenda
Sugie, M.; Suzuki, H.
Optical resolution of DL-amino acids with D-Aminoacylase of Streptomyces
Agric. Biol. Chem.
44
1089-1095
1980
Streptomyces sp., no activity in Streptomyces olivaceus, no activity in Streptomyces olivaceus IFO 12805
-
brenda
Szajani, B.; Ivony, K.; Boross, L.
Comparative studies on soluble and immobilized pig kidney aminoacylase
J. Appl. Biochem.
2
72-80
1980
Aspergillus oryzae, Sus scrofa
-
brenda
Gade, W.; Brown, J.L.
Purification, characterization and possible function of alpha-N-acylamino acid hydrolase from bovine liver
Biochim. Biophys. Acta
662
86-93
1981
Bos taurus, Sus scrofa
brenda
Gilles, I.; Loeffler, H.G.; Schneider, F.
Co2+-substituted acylamino acid amido hydrolase from Aspergillus oryzae
Z. Naturforsch. C
36
751-754
1981
Aspergillus oryzae
-
brenda
Kumpe, E.; Lffler, H.G.; Schneider, F.
Studies on the Zn2+/Co2+ exchange with acylamino acid amidohydrolase from pig kidney
Z. Naturforsch. C
36
951-955
1981
Sus scrofa
brenda
Cho, H.Y.; Tanizawa, K.; Tanaka, H.; Soda, K.
Thermostable aminoacylase from Bacillus thermoglucosidius: Purification and characterization
Agric. Biol. Chem.
51
2793-2800
1987
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Sus scrofa
-
brenda
Henseling, J.; Roehm, K.H.
Unusual solvent isotope effects on the aminoacylase-catalyzed hydrolysis of acetylamino acids
FEBS Lett.
219
27-30
1987
Sus scrofa
brenda
Sakanyan, V.; Desmarez, L.; Legrain, C.; Charlier, D.; Mett, I.; Kochikyan, A.; Savchenko, A.; Boyen, A.; Falmagne, P.; Pierard, A.; Glansdorff, N.
Gene cloning, sequence analysis, purification, and characterization of an thermostable aminoacylase from Bacillus stearothermophilus
Appl. Environ. Microbiol.
59
3878-3888
1993
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Homo sapiens, Sus scrofa
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Aspergillus oryzae
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Homo sapiens, Sus scrofa (P37111), Sus scrofa
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Thermococcus litoralis
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Pyrococcus horikoshii (O58754), Pyrococcus horikoshii, Pyrococcus horikoshii OT-3 (O58754)
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2002
Thermococcus litoralis
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Thermococcus litoralis
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Homo sapiens (Q03154), Homo sapiens
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Homo sapiens (Q03154)
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Geobacillus sp., Geobacillus sp. SZN
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Sus scrofa
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