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Literature summary for 3.5.1.14 extracted from

  • Durand, A.; Giardina, T.; Villard, C.; Roussel, A.; Puigserver, A.; Perrier, J.
    Rat kidney acylase I: further characterization and mutation studies on the involvement of Glu 147 in the catalytic process (2003), Biochimie, 85, 953-962.
    View publication on PubMed
Search for more literature for 3.5.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E147A completely inactive Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Rattus norvegicus
45847
-
 3 * 45847, deduced from deduced aminacid sequence Rattus norvegicus
125000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
by a method that includes DEAE-Sepharose column, Chelating-Sepharose column, Amicon PM 30 membrane and Sephacryl-S200 column Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
-
 Rattus norvegicus
8.33
-
 pH 8.0, 37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-methionine + H2O
-
 Rattus norvegicus acetate + L-methionine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, denaturing and reducing gels Rattus norvegicus
trimer 3 * 44000, gel filtration and SDS-PAGE Rattus norvegicus
trimer 3 * 45847, deduced from deduced aminacid sequence Rattus norvegicus