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Literature summary for 3.5.1.14 extracted from
- Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii (2008), FEBS J., 275, 1140-1149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibits PhoACY activity | Pyrococcus horikoshii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.6 | - |
N-acetyl-L-methionine | - |
Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 165000 | - |
under native conditions, homotetrameric structure | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O58453 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| in 50 mM Tris/HCl-buffer, pH 8 and disrupted by ultrasonication | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-methionine + H2O | - |
Pyrococcus horikoshii | acetate + L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4* 42000 SDS-PAGE, recombinant enzyme | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhoACY | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus horikoshii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | - |
Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 370 | - |
N-acetyl-L-methionine | - |
Pyrococcus horikoshii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pyrococcus horikoshii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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