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Literature summary for 3.5.1.14 extracted from

  • Liu, Z.; Zhen, Z.; Zuo, Z.; Wu, Y.; Liu, A.; Yi, Q.; Li, W.
    Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking (2006), J. Biochem., 139, 421-430.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in E. coli Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
N-alpha-acetyl-L-methionine native porcine enzyme,25°C Sus scrofa
1.2
-
N-alpha-acetyl-L-methionine E309H-mutant, 25°C Sus scrofa
1.25
-
N-alpha-acetyl-L-methionine Wild type Rosetta (DE3),25°C Sus scrofa
2.12
-
N-alpha-acetyl-L-methionine R274A-mutant, 25°C Sus scrofa
2.21
-
N-alpha-acetyl-L-methionine H79A-mutant, 25°C Sus scrofa
2.23
-
N-alpha-acetyl-L-methionine E147A-mutant, 25°C Sus scrofa
2.36
-
N-alpha-acetyl-L-methionine E174A-mutant, 25°C Sus scrofa
2.45
-
N-alpha-acetyl-L-methionine H371A-mutant, 25°C Sus scrofa
2.52
-
N-alpha-acetyl-L-methionine D112A-mutant, 25°C Sus scrofa
3.82
-
N-alpha-acetyl-L-methionine H205L-mutant, 25°C Sus scrofa
4.34
-
N-alpha-acetyl-L-methionine E146A-mutant, 25°C Sus scrofa
4.57
-
N-alpha-acetyl-L-methionine N261A-mutant, 25°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa P37111
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
H79A-mutant, 25°C Sus scrofa
9.2
-
N261A-mutant, 25°C Sus scrofa
9.4
-
D112A-mutant, 25°C Sus scrofa
10.3
-
Wild type BL21 (DE3), 25°C Sus scrofa
13.6
-
E146A-mutant, 25°C Sus scrofa
25.7
-
E147A-mutant, 25°C Sus scrofa
32.6
-
H371A-mutant, 25°C Sus scrofa
53.6
-
E174A-mutant, 25°C Sus scrofa
78.5
-
H205L-mutant, 25°C Sus scrofa
197
-
R274A-mutant, 25°C Sus scrofa
203
-
E309H-mutant, 25°C Sus scrofa
220
-
Wild type Rosetta (DE3), 25°C Sus scrofa
287
-
native porcine enzyme, 25°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-methionine + H2O
-
Sus scrofa acetate + L-methionine
-
?

Synonyms

Synonyms Comment Organism
pAcy1
-
Sus scrofa