Cloned (Comment) | Organism |
---|---|
expression in E. coli | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
N-alpha-acetyl-L-methionine | native porcine enzyme,25°C | Sus scrofa | |
1.2 | - |
N-alpha-acetyl-L-methionine | E309H-mutant, 25°C | Sus scrofa | |
1.25 | - |
N-alpha-acetyl-L-methionine | Wild type Rosetta (DE3),25°C | Sus scrofa | |
2.12 | - |
N-alpha-acetyl-L-methionine | R274A-mutant, 25°C | Sus scrofa | |
2.21 | - |
N-alpha-acetyl-L-methionine | H79A-mutant, 25°C | Sus scrofa | |
2.23 | - |
N-alpha-acetyl-L-methionine | E147A-mutant, 25°C | Sus scrofa | |
2.36 | - |
N-alpha-acetyl-L-methionine | E174A-mutant, 25°C | Sus scrofa | |
2.45 | - |
N-alpha-acetyl-L-methionine | H371A-mutant, 25°C | Sus scrofa | |
2.52 | - |
N-alpha-acetyl-L-methionine | D112A-mutant, 25°C | Sus scrofa | |
3.82 | - |
N-alpha-acetyl-L-methionine | H205L-mutant, 25°C | Sus scrofa | |
4.34 | - |
N-alpha-acetyl-L-methionine | E146A-mutant, 25°C | Sus scrofa | |
4.57 | - |
N-alpha-acetyl-L-methionine | N261A-mutant, 25°C | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P37111 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.4 | - |
H79A-mutant, 25°C | Sus scrofa |
9.2 | - |
N261A-mutant, 25°C | Sus scrofa |
9.4 | - |
D112A-mutant, 25°C | Sus scrofa |
10.3 | - |
Wild type BL21 (DE3), 25°C | Sus scrofa |
13.6 | - |
E146A-mutant, 25°C | Sus scrofa |
25.7 | - |
E147A-mutant, 25°C | Sus scrofa |
32.6 | - |
H371A-mutant, 25°C | Sus scrofa |
53.6 | - |
E174A-mutant, 25°C | Sus scrofa |
78.5 | - |
H205L-mutant, 25°C | Sus scrofa |
197 | - |
R274A-mutant, 25°C | Sus scrofa |
203 | - |
E309H-mutant, 25°C | Sus scrofa |
220 | - |
Wild type Rosetta (DE3), 25°C | Sus scrofa |
287 | - |
native porcine enzyme, 25°C | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-methionine + H2O | - |
Sus scrofa | acetate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pAcy1 | - |
Sus scrofa |